Ubiquitin Proteasome System Activity is Suppressed by Curcumin following Exercise-Induced Muscle Damage in Human Skeletal Muscle

Cardaci, Thomas D.; Machek, Steven B.; Wilburn, Dylan T.; Hwang, Paul S; Willoughby, Darryn S.

doi:10.1080/07315724.2020.1783721

Cited by 24 publications

(21 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All extracts were supplemented with phenylmethanesulfonyl fluoride and a protease inhibitor cocktail (Sigma Chemical Company, St. Louis, MO, USA) with broad specificity for the inhibition of serine, cysteine, and metallo-proteases. Protein contents for total protein, sarcoplasm-, and nuclear-extracted samples were analyzed in duplicate and determined spectrophotometrically at a wavelength of 750 nm (Bio-Rad Hercules, CA, USA) and using bovine serum albumin as the standard [ 49 ].…”

Section: Methodsmentioning

confidence: 99%

High-Load Resistance Exercise Augments Androgen Receptor–DNA Binding and Wnt/β-Catenin Signaling without Increases in Serum/Muscle Androgens or Androgen Receptor Content

et al. 2020

Self Cite

View full text Add to dashboard Cite

The purpose of this study was (1) to determine the effect of single bouts of volume- and intensity-equated low- (LL) and high-load (HL) full-body resistance exercise (RE) on AR-DNA binding, serum/muscle testosterone and dihydrotestosterone, muscle androgen receptor (AR), and AR-DNA binding; and, (2) to determine the effect of RE on sarcoplasmic and nucleoplasmic β-catenin concentrations in order to determine their impact on mediating AR-DNA binding in the absence/presence of serum/muscle androgen and AR protein. In a cross-over design, 10 resistance-trained males completed volume- and intensity-equated LL and HL full-body RE. Blood and muscle samples were collected at pre-, 3 h-, and 24 h post-exercise. Separate 2 × 3 factorial analyses of variance (ANOVAs) with repeated measures and pairwise comparisons with a Bonferroni adjustment were used to analyze the main effects. No significant differences were observed in muscle AR, testosterone, dihydrotestosterone, or serum total testosterone in either condition (p > 0.05). Serum-free testosterone was significantly decreased 3 h post-exercise and remained significantly less than baseline 24 h post-exercise in both conditions (p < 0.05). In response to HL, AR-DNA binding significantly increased at 3 h post-exercise (p < 0.05), whereas no significant differences were observed at any time in response to LL (p > 0.05). Moreover, sarcoplasmic β-catenin was significantly greater in HL (p < 0.05) without significant changes in nucleoplasmic β-catenin (p > 0.05). In conclusion, increases in AR-DNA binding in response to HL RE indicate AR signaling may be load-dependent. Furthermore, despite the lack of increase in serum and muscle androgens or AR content following HL RE, elevations in AR-DNA binding with elevated sarcoplasmic β-catenin suggests β-catenin may be facilitating this response.

show abstract

Section: Methodsmentioning

confidence: 99%

High-Load Resistance Exercise Augments Androgen Receptor–DNA Binding and Wnt/β-Catenin Signaling without Increases in Serum/Muscle Androgens or Androgen Receptor Content

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…10,31,32,36,[52][53][54][55][56][57][58][59] Only 7 studies definitively reported lidocaine was administered without epinephrine. 23,51,[60][61][62][63][64] The majority of the authors did not report lidocaine administration with or without epinephrine (~77%). Five studies reported the use of a superficial lidocaine/prilocaine cream or lidocaine tape as a local anesthetic prior to the microbiopsy procedure.…”

Section: Resultsmentioning

confidence: 99%

“…Twelve studies (~14%) reported the use of epinephrine with lidocaine administration 10,31,32,36,52–59 . Only 7 studies definitively reported lidocaine was administered without epinephrine 23,51,60–64 . The majority of the authors did not report lidocaine administration with or without epinephrine (~77%).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The skeletal muscle microbiopsy method in exercise and sports science research: A narrative and methodological review

Newmire

Willoughby

2022

Scandinavian Med Sci Sports

Self Cite

View full text Add to dashboard Cite

Background The skeletal muscle microbiopsy protocol was introduced to the Exercise and Sports Science (ESS) research field in 1999 and has been used as a protocol to directly examine muscular structural and biochemical changes. There is much variation in the reporting of the microbiopsy protocol and its related pre‐ and post‐procedure for participant care and sample collection. The purpose of this narrative and methodological review is to compare the microbiopsy to the traditional Bergström protocol used in the ESS field, identify and summarize all related microbiopsy protocols used in previous ESS studies and determine the most frequently used microbiopsy protocols aspects and associated pre‐ and post‐biopsy procedures. Methods A review of literature up to January 2022 was used following the PRISMA and Cochrane Methodological Review Guide to determine frequently used methods that may facilitate optimal and potential recommendations for muscle microbiopsy needle gauge (G), concentration or dose (% or ml) and administration of local anesthetic, co‐axial/cannula introducer gauge (G), muscle depth (cm), muscle sample size collected (mg), passes to collect samples, time points of muscle sampling, and promotion of participant compliance and minimization of adverse events. Results Eighty‐five articles were selected based on the inclusionary requirements related to the ESS field or methodological considerations. The most frequently reported aspects in previous research to suggest the location of the vastus lateralis is the midpoint between the patella and the greater trochanter of the femur or 1/3 or 2/3 the distance from the patella to anterior superior iliac spine, 14 G biopsy needle, subcutaneous injected lidocaine administration (2 ml, 1%), 13 G co‐axial/cannula, 1–2 cm muscle depth, 10–20 mg of muscle sample, ~3‐time points, and 2–3 passes. Discussion There is much variation in the reporting of the microbiopsy protocol and its related pre‐ and post‐biopsy procedures. Standardization in reporting may promote recommendations to optimize data integrity, participant safety, participant adherence to the study design, and increase reproducibility. Recommendations are made for the microbiopsy procedure based on frequently reported characteristics.

show abstract

“…As the public's interest on natural phytotherapy and diet therapy related to health and disease increases, this attractive and safe alternative has been extensively studied. Previous studies have also shown that curcumin alleviates postexercise skeletal muscle injury by inhibiting the activity of the ubiquitin-proteasome system [ 26 ]. Therefore, we hypothesize that curcumin may be able to improve breast cancer cachexia-related skeletal muscle atrophy.…”

Section: Introductionmentioning

confidence: 99%

Curcumin Targeting NF-κB/Ubiquitin-Proteasome-System Axis Ameliorates Muscle Atrophy in Triple-Negative Breast Cancer Cachexia Mice

Zhang

Zheng

Chen

et al. 2022

Mediators of Inflammation

View full text Add to dashboard Cite

Background. Curcumin is a polyphenol plant-derived compound with anti-inflammatory, antioxidant stress, and anticancer properties that make it have the potential to treat cancer cachexia. However, the role of it in breast cancer cachexia remains unclear. Methods. The 4T1 cells were subcutaneously injected into BALB/c mice to induce breast cancer cachexia. After tumor formation, the animals were divided into groups and given curcumin or saline interventions. The therapeutic effect of curcumin on breast cancer cachexia was characterized by tumor growth, changes in body mass and gastrocnemius mass, muscle function test, histopathology, and serum nutrition indexes. Mitochondrial function in muscle tissue was observed by transmission electron microscopy and ATP detection, muscle inflammatory factors were detected by ELISA, muscle differential metabolites were detected by 1HNMR metabolomics, and the muscle tissue ubiquitination levels and NF-KB expression were also analyzed by RT-qPCR and Western blot. Results. Dynamic in vivo bioluminescence imaging find that curcumin inhibited the growth of tumor in triple-negative breast cancer- (TNBC-) bearing mice, slowed down the loss of body weight and gastrocnemius weight, corrected the mitochondrial dysfunction and malnutrition status, and also significantly improved skeletal muscle function. ELISA analysis found that the level of inflammatory factors in muscle tissue was reduced. 1HNMR metabolomics analysis suggested that curcumin could regulate energy metabolism pathways. RT-qPCR and Western blot analysis found that the expression of myogenic factor myogenin was increased and the expression of myodegradation factor myostatin was decreased in the gastrocnemius; the level of ubiquitination and activation of the NF-κB pathway were also declined. Conclusions. Curcumin reduces ubiquitination, inflammation in skeletal muscle by regulating the NF-KB/UPS axis and improves muscle malignant metabolic phenotype and mitochondrial dysfunction, to alleviate muscle atrophy and loss of function in mice with breast cancer cachexia.

show abstract

Ubiquitin Proteasome System Activity is Suppressed by Curcumin following Exercise-Induced Muscle Damage in Human Skeletal Muscle

Cited by 24 publications

References 47 publications

High-Load Resistance Exercise Augments Androgen Receptor–DNA Binding and Wnt/β-Catenin Signaling without Increases in Serum/Muscle Androgens or Androgen Receptor Content

High-Load Resistance Exercise Augments Androgen Receptor–DNA Binding and Wnt/β-Catenin Signaling without Increases in Serum/Muscle Androgens or Androgen Receptor Content

The skeletal muscle microbiopsy method in exercise and sports science research: A narrative and methodological review

Curcumin Targeting NF-κB/Ubiquitin-Proteasome-System Axis Ameliorates Muscle Atrophy in Triple-Negative Breast Cancer Cachexia Mice

Contact Info

Product

Resources

About