Ubiquitin-specific peptidase 46 promotes tumor metastasis through stabilizing ENO1 in human esophageal squamous cell carcinoma

Tian, Maoqing; Zhu, Rui; Ding, Fang; Liu, Zhihua

doi:10.1016/j.yexcr.2020.112188

Cited by 20 publications

(7 citation statements)

References 37 publications

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“…Ubiquitination acts as one of the post translational modifications of protein, which eliminates misfolded proteins and regulates different signaling pathways and cell functions. [33][34][35] The ubiquitination level of protein relies on the synergistical effects of its ubiquitinases and deubiquitinases. 36,37 In recent years, an increasing number of studies have reported that the deubiquitinating enzymes (DUBs) play important roles in cancer development and therapeutic resistance.…”

Section: Discussionmentioning

confidence: 99%

USP21 promotes cell proliferation by maintaining the EZH2 level in diffuse large B‐cell lymphoma

Luo

Zhou

et al. 2021

Clinical Laboratory Analysis

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Background: Diffuse large B-cell lymphoma (DLBCL) is the most common category of non-Hodgkin lymphoma (NHL). However, the underlying molecular mechanism of DLBCL remains unclear. Methods: Real-time PCR and Western blot analysis were performed to assess the expression of ubiquitin-specific peptidase 21 (USP21) or enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2). CCK8 assay and cell death staining were carried out to examine the role of USP21 in cell proliferation and cell death, respectively. Results: We found that the deubiquitinase USP21 was highly expressed in the DLBCL lymphoid tissue. The expression of USP21 promoted DLBCL cell proliferation, while it had no obvious effect on cell death. In addition, we found that USP21 regulated cell proliferation via cysteine 221, the catalytic site of USP21. Furthermore, we identified that USP21 could stabilize EZH2, a protein required for germinal center formation and lymphoma formation. Conclusion: The deubiquitinase USP21 promotes cell proliferation by maintaining the EZH2 protein level in DLBCL.

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Section: Discussionmentioning

confidence: 99%

USP21 promotes cell proliferation by maintaining the EZH2 level in diffuse large B‐cell lymphoma

Luo

Zhou

et al. 2021

Clinical Laboratory Analysis

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“…Inhibiting tumor metastasis [422][423][424] USP35 Suppressor ABIN-2 Stabilization Inhibit proliferation [ 425] USP36 Promotor c-Myc, DHX33, CHD7, H2B Altered activity or stabilization Promote tumorigenesis and proliferation [426][427][428][429] USP37 Promotor RARA, Gli1, Snail, 14-3-3𝛾, c-Myc Stabilization Promote proliferation and metastasis [430][431][432][433][434] Suppressor p27 Stabilization Inhibit proliferation [ 435] USP42 Suppressor p53 Stabilization Inhibit proliferation [ 436] USP43 Promotor H2B, ZEB1 Altered activity or Stabilization Promote proliferation and metastasis [ 156,437] USP44 Promotor H2B, EZH2, Securin Altered activity or Stabilization Promote proliferation and metastasis [438][439][440][441] USP46 Promotor CDT2, ENO1 Stabilization Promote proliferation and metastasis [ 442,443] Suppressor PHLPP Stabilization Inhibit proliferation [ 444] USP47 Promotor 𝛽-catenin, Snail Stabilization Promote proliferation and metastasis [445][446][447] USP48 Promotor TRAF2, Gli1 Stabilization Promote tumorigenesis and metastasis [ 448] USP49 Suppressor p53, FKBP51 Stabilization Inhibit proliferation and regulate DDR [449,450] USP51 Promotor ZEB1, FAT4 Stabilization Promote proliferation and metastasis [ 451,452] USP52 Promotor ASF1A Stabilization Drug resistance [ 453] CYLD Suppressor Dvl, p53, TRAF2, Bcl-3, c-Jun, c-Fox Stabilization Suppress tumorigenesis and metastasis, promote apoptosis, regulate DDR [454][455]…”

Section: Altered Activity or Stabilizationmentioning

confidence: 99%

Deubiquitylating Enzymes in Cancer and Immunity

Ren,

Yu,

Liu

et al. 2023

Advanced Science

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Deubiquitylating enzymes (DUBs) maintain relative homeostasis of the cellular ubiquitome by removing the post‐translational modification ubiquitin moiety from substrates. Numerous DUBs have been demonstrated specificity for cleaving a certain type of ubiquitin linkage or positions within ubiquitin chains. Moreover, several DUBs perform functions through specific protein–protein interactions in a catalytically independent manner, which further expands the versatility and complexity of DUBs’ functions. Dysregulation of DUBs disrupts the dynamic equilibrium of ubiquitome and causes various diseases, especially cancer and immune disorders. This review summarizes the Janus‐faced roles of DUBs in cancer including proteasomal degradation, DNA repair, apoptosis, and tumor metastasis, as well as in immunity involving innate immune receptor signaling and inflammatory and autoimmune disorders. The prospects and challenges for the clinical development of DUB inhibitors are further discussed. The review provides a comprehensive understanding of the multi‐faced roles of DUBs in cancer and immunity.

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“…As a tumor suppressor gene, it can antagonize the activity of AKT by deubiquitinating the PH domain leucine-rich repeat protein phosphatase 1 (PHLPP1), thereby inhibiting the proliferation of cancer cells (Gui et al, 2019;Li et al, 2013;Wang et al, 2020). In esophageal squamous cell carcinoma, Tian et al confirmed the over-expression of USP46 by analyzing clinical samples, and in vitro studies confirmed that USP46 enhances the migration and invasion of esophageal squamous cell carcinoma cells by mediating the EMT process (Tian et al, 2020). By knocking down the expression of USP46, it can effectively inhibit the CDX tumor growth of HPV-related cervical cancer (Kiran et al, 2018).…”

Section: T a B L E 2 Analyses Of Univariate And Multivariate On Mir-2...mentioning

confidence: 99%

MiR‐27a‐3p targets USP46 to inhibit the cell proliferation of hepatocellular carcinoma

Wen

Peng

et al. 2022

Chem Biol Drug Des

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Micro‐RNAs are involved in the occurrence and development of hepatocellular carcinoma (HCC) as potential therapeutic targets for HCC. In this study, we found that miR‐27a‐3p was highly expressed in HCC, which was associated with lower survival rates of HCC patients. In vivo and in vitro functional experiments confirmed that over‐expression or knock‐down miR‐27a‐3p could significantly affect the proliferation ability of HCCLM3 and Huh‐7, two HCC cell lines. Ubiquitin‐specific protease 46 (USP46) was confirmed as the key target gene of miR‐27a‐3p in HCC via RNA‐seq, quantitative polymerase chain reaction, Western blotting, and luciferase report. When knocking down USP46, the proliferation activity of HCC cells was significantly enhanced, while it was significantly inhibited after over‐expressing USP4. Above results suggest that the abnormally over‐expressed miR‐27a‐3p in liver promotes the proliferation of cancer cells and accelerates the development of HCC by targeting inhibition the expression of USP46. Targeting miR‐27a‐3p may be an effective strategy for prevention and treatment of HCC.

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Ubiquitin-specific peptidase 46 promotes tumor metastasis through stabilizing ENO1 in human esophageal squamous cell carcinoma

Cited by 20 publications

References 37 publications

USP21 promotes cell proliferation by maintaining the EZH2 level in diffuse large B‐cell lymphoma

USP21 promotes cell proliferation by maintaining the EZH2 level in diffuse large B‐cell lymphoma

Deubiquitylating Enzymes in Cancer and Immunity

MiR‐27a‐3p targets USP46 to inhibit the cell proliferation of hepatocellular carcinoma

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