Ubiquity of lignin-degrading peroxidases among various wood-degrading fungi

Abstract

Phanerochaete chrysosporium is rapidly becoming a model system for the study of lignin biodegradation. Numerous studies on the physiology, biochemistry, chemistry, and genetics of this system have been performed. However, P. chrysosporium is not the only fungus to have a lignin-degrading enzyme system. Many other ligninolytic species of fungi, as well as other distantly related organisms which are known to produce lignin peroxidases, are described in this paper. In this study, we demonstrated the presence of the peroxidative enzymes in nine species not previously investigated. The fungi studied produced significant manganese peroxidase activity when they were grown on an oak sawdust substrate supplemented with wheat bran, millet, and sucrose. Many of the fungi also exhibited laccase and/or glyoxal oxidase activity. Inhibitors present in the medium prevented measurement of lignin peroxidase activity. However, Western blots (immunoblots) revealed that several of the fungi produced lignin peroxidase proteins. We concluded from this work that lignindegrading peroxidases are present in nearly all ligninolytic fungi, but may be expressed differentially in different species. Substantial variability exists in the levels and types of ligninolytic enzymes produced by different white rot fungi. Recent reports have raised the issue of the involvement of LPs in the degradation of lignin (45). These reports have

Molecular characterization of a novel peroxidase isolated from the ligninolytic fungus Pleurotus eryngii

“…It is interesting that the identity between the peroxidase MnPL and the MnP isoenzymes of P. chrysosporium (55%) is lower than found with the LiP of the same fungus (60% and 58% with isoenzymes H2 and H8 respectively). This could justify the reaction of P. eryngii peroxidases with antibodies against P. chrysosporium LiP reported by Orth et al (1993). However, differences between P. eryngii and P. chrysosporium peroxidase genes were shown by the lack of hybridization of P. chrysosporium DNA with a probe including a P. eryngii peroxidase gene compared with cross-hybridization between P. eryngii and P. ostreatus (Sarkar et al, 1997).…”

A study on reducing substrates of manganese‐oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta

“…Recently a similar peroxidase was detected in B. adusta, a fungus selected for biodegradation of industrial dyes [8], together with LiP already described by Kimura et al [9]. Despite some reports suggesting LiP production by Pleurotus species [10,11], a typical LiP has not been found in this genus. Since the ability to oxidize aromatic compounds directly, i.e.…”

Screening for basidiomycetous fungi capable of degrading 2,7-dichlorodibenzo-p-dioxin

“…Nevertheless, dioxins tend to remain in the culture despite incubations as long as 1 month, even with Phanerochaete chrysosporium [1,2], a fungus frequently used in bioremediation studies. Previous studies [7,8] suggested the existence of several fungi more active than P. chrysosporium. We therefore anticipated that we would be able to ¢nd e¡ective dioxin-degrading fungi by screening a variety of isolates and natural samples.…”