2004
DOI: 10.1074/jbc.m406926200
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Ubiquitylation of Neuronal Nitric-oxide Synthase by CHIP, a Chaperone-dependent E3 Ligase

Abstract: It is established that neuronal nitric-oxide synthase (nNOS) is ubiquitylated and proteasomally degraded. The proteasomal degradation of nNOS is enhanced by suicide inactivation of nNOS or by the inhibition of hsp90, which is a chaperone found in a native complex with nNOS. In the current study, we have examined whether CHIP, a chaperone-dependent E3 ubiquitinprotein isopeptide ligase that is known to ubiquitylate other hsp90-chaperoned proteins, could act as an ubiquitin ligase for nNOS. We found with the use… Show more

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Cited by 69 publications
(95 citation statements)
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“…Neither mutant shows a significant increase in response to GSNO. The enhanced basal nitrosylation of wild type CLIC4, in the presence of lactacystin, is probably a result of enhanced stabilization and activity of NOS enzymes (27,28). These results taken together suggest that cysteines 35 and 189, which are proximal to cysteine 234 in the crystal structure (supplemental Fig.…”
Section: Nitric Oxide Induces Nuclear Translocation Of Clic4 In Normasupporting
confidence: 52%
“…Neither mutant shows a significant increase in response to GSNO. The enhanced basal nitrosylation of wild type CLIC4, in the presence of lactacystin, is probably a result of enhanced stabilization and activity of NOS enzymes (27,28). These results taken together suggest that cysteines 35 and 189, which are proximal to cysteine 234 in the crystal structure (supplemental Fig.…”
Section: Nitric Oxide Induces Nuclear Translocation Of Clic4 In Normasupporting
confidence: 52%
“…The C terminus of Hsc70-interacting protein (CHIP) has been shown to be an E3 ligase that ubiquitinates cytochromes P450 3A4 and 2E1 as well as nNOS (10 -12). The ubiquitination of nNOS by CHIP is enhanced in the presence of Hsp70 (8,10). Moreover, suicide-inactivated nNOS was found to be selectively ubiquitinated by this Hsp70/CHIP system (8).…”
mentioning
confidence: 83%
“…CHIP mediates the ubiquitination and degradation of several proteins. including Raf1 (39) and neuronal nitric-oxide synthase (40). Given its ability to up-regulate CHIP, it is not surprising that hyperglycemia was able to promote GATA4 degradation even when it paradoxically inhibited the caspase-like activity in the 20 S proteasome.…”
Section: Discussionmentioning
confidence: 99%