In this research, it was aimed to develop a valid and reliable test consisting of multiple choice questions in order to measure the conceptual understanding of the "cell" unit, which is one of the biology course topics. Planning, item writing, item analysis and item selection were followed in the development of the achievement test. Within the scope of the biology course, an item pool of 29 questions covering the cell topic was created. With the expert opinions, the content validity of the questions was ensured and the questions were corrected. Item analyzes were made with the pilot application of the draft form. As a result of item analysis, the number of questions was reduced to 22. After the item analyzes carried out, the “Cell Achievement Test”, consisting of a total of 22 questions for the "cell" unit, took its final form. While the average item difficulty of the test was calculated as 0.55, the average item discrimination was calculated as 0.44. Moreover, the test was applied to a different group of 123 undergraduate students for reliability analysis, and the KR-20 reliability coefficient was calculated as 0.89. As a result of the findings, a valid and reliable "Cell Achievement Test" was obtained.