ObjectiveThe current research was carried out to test the validity and reliability of the Turkish version of the Acute Stroke Management Questionnaire (ASMaQ), developed to determine the acute stroke management awareness of health‐care professionals.MethodsThis methodological study was performed in a training and research hospital. Data were collected using the “Participant Interview Form” and the Acute Stroke Management Questionnaire.ResultsThe Content Validity Index (CVI) of the Acute Stroke Management Questionnaire was found to be 0.91. As a result of exploratory factor analysis (EFA), it was revealed that the scale, which was adapted to Turkish, consisted of 3‐factor and 29 items, as in its original form. Confirmatory factor analysis confirmed the 3‐factor structure of the scale. The ASMaQ exhibited strong internal consistency (Cronbach's alpha 0.96) and test–retest reliability (0.879).ConclusionThe 29‐item and three‐dimensional structure of the Acute Stroke Management Questionnaire was revealed to be a valid and reliable measurement tool that could be utilized in evaluating the acute stroke management awareness of health‐care professionals in the Turkish language and culture.