UDP-glucose pyrophosphorylase gene affects mycelia growth and polysaccharide synthesis of Grifola frondosa

Zan, Xinyi; Wu, Xi-Hong; Cui, Fengjie; Zhu, Hong-An; Sun, Wenjing; Jiang, Lihua; Tao, Ting-Lei; Zhao, Xuyao

doi:10.1016/j.ijbiomac.2020.06.139

Cited by 23 publications

(10 citation statements)

References 35 publications

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“…It can be converted into glucose-1-phosphate, fructose-6-phosphate by phosphoglucomutase, glucose-6-phosphate isomerase, and then glucose-1-phosphate to generate UDP-glucose (the glycosyl donor unit of polysaccharides structure) by uridylyltransferase [ 30 ]. UDP-glucose can also be further converted into other glycosyl donors, such as UDP-Gal and UDP-GlcA [ 31 , 32 ]. In addition, fructose-6-phosphate can be converted into UDP-N-acetylglucosamine (UDP-GlcNAc) by UDP-N-acetylglucosamine pyrophosphorylase [ 33 ].…”

Section: Resultsmentioning

confidence: 99%

Enhancement of polysaccharides production using microparticle enhanced technology by Paraisaria dubia

Tong

Wang

et al. 2022

Microb Cell Fact

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Background Polysaccharides are important active ingredients in Ophiocordyceps gracilis with many physiological functions. It can be obtained from the submerged fermentation by the anamorph (Paraisaria dubia) of Ophiocordyceps gracilis. However, it was found that the mycelial pellets of Paraisaria dubia were dense and increased in volume in the process of fermentation, and the center of the pellets was autolysis due to the lack of nutrient delivery, which extremely reduced the yield of polysaccharides. Therefore, it is necessary to excavate a fermentation strategy based on morphological regulation for Paraisaria dubia to promote polysaccharides accumulation. Results In this study, we developed a method for enhancing polysaccharides production by Paraisaria dubia using microparticle enhanced technology, talc microparticle as morphological inducer, and investigated the enhancement mechanisms by transcriptomics. The optimal size and dose of talc were found to be 2000 mesh and 15 g/L, which resulted in a high polysaccharides yield. It was found that the efficient synthesis of polysaccharides requires an appropriate mycelial morphology through morphological analysis of mycelial pellets. And, the polysaccharides synthesis was found to mainly rely on the ABC transporter-dependent pathway revealed by transcriptomics. This method was also showed excellent robustness in 5-L bioreactor, the maximum yields of intracellular polysaccharide and exopolysaccharides were 83.23 ± 1.4 and 518.50 ± 4.1 mg/L, respectively. And, the fermented polysaccharides were stable and showed excellent biological activity. Conclusions This study provides a feasible strategy for the efficient preparation of cordyceps polysaccharides via submerged fermentation with talc microparticles, which may also be applicable to similar macrofungi. Graphical Abstract

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Section: Resultsmentioning

confidence: 99%

Enhancement of polysaccharides production using microparticle enhanced technology by Paraisaria dubia

Tong

Wang

et al. 2022

Microb Cell Fact

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“…The cell wall provides mechanical support for the cells during growth and development. Hyphal prolonging and branching are positively correlated with the continuous synthesis of the cell wall [24]. The knockdown of GlbHLH significantly decreased the contents of β-1,3-glucans and chitins in the cell wall by reducing the expression of related synthesis genes, thereby leading to the impaired growth and morphological changes of G. lucidum.…”

Section: Discussionmentioning

confidence: 99%

Transcription factor GlbHLH regulates hyphal growth, stress resistance, and polysaccharide biosynthesis in Ganoderma lucidum

Ying-yin

Zhang

et al. 2021

J Basic Microbiol

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Basic helix-loop-helix (bHLH) transcription factors (TFs) participate in many physiological and cellular processes in eukaryotes. However, their functions remain unclear in the macro basidiomycete Ganoderma lucidum (G. lucidum).In this study, a gene encoding bHLH TF, GlbHLH, was identified in G. lucidum. The knockdown of GlbHLH by RNA interference reduced hyphal growth, hyphal branching, and resistant to osmotic, oxidative, and cell wall stress. The content of cell wall components β-1,3 glucan and chitin and the expression of their synthesis genes were decreased in the GlbHLH knockdown strains. The knockdown of GlbHLH led to an increase of intracellular reactive oxygen species by decreasing the enzyme activity and gene expression of antioxidant enzymes. Furthermore, the production of intracellular polysaccharides and extracellular polysaccharides was greatly decreased in the GlbHLH mutants. These results suggested that GlbHLH is involved in hyphal growth, stress response, and polysaccharide biosynthesis in G. lucidum.

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“…Mitochondrial import inner membrane translocase was highly expressed in the Pri stage compared with the Knot and Rec stages. UDP-glucose pyrophosphorylase (UGP: UDP-xylose-4-epimerase and UDP-glucose-4-epimerase) plays a significant role in mycelial growth and polysaccharide synthesis in the development of mushrooms [ 34 ]. UDP-glucose pyrophosphorylase was highly expressed in the Pri stage compared with the Knot stage.…”

Section: Discussionmentioning

confidence: 99%

Comparative Proteomic Analyses within Three Developmental Stages of the Mushroom White Hypsizygus marmoreus

Lin

et al. 2023

JoF

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(1) Background: The Hypsizygus marmoreus is a popular edible mushroom in East Asian markets. In a previous study, we reported the proteomic analyses of different developmental stages of H. marmoreus, from primordium to mature fruiting body. However, the growth and protein expression changes from scratching to primordium are unclear. (2) Methods: A label-free LC-MS/MS quantitative proteomic analysis technique was adopted to obtain the protein expression profiles of three groups of samples collected in different growth stages from scratching to the tenth day after scratching. The Pearson’s correlation coefficient analysis and principal component analysis were performed to reveal the correlation among samples. The differentially expressed proteins (DEPs) were organized. Gene Ontology (GO) analysis was performed to divide the DEPs into different metabolic processes and pathways. (3) Results: From the 3rd day to the 10th day after scratching, mycelium recovered gradually and formed primordia. Compared with the Rec stage, 218 highly expressed proteins were identified in the Knot stage. Compared with the Pri stage, 217 highly expressed proteins were identified in the Rec stage. Compared with the Pri stage, 53 highly expressed proteins were identified in the Knot stage. A variety of the same highly expressed proteins were identified in these three developmental stages, including: glutathione S-transferase, acetyltransferase, importin, dehydrogenase, heat-shock proteins, ribosomal proteins, methyltransferase, etc. The key pathways in the development of H. marmoreus are metabolic process, catabolic process, oxidoreductase activity and hydrolase activity. DEPs in the Knot or Pri stages compared with the Rec stage were significantly decreased in the metabolic-, catabolic- and carbohydrate-related process; and the oxidoreductase, peptidase, and hydrolase activity, which can serve as targets for selectable molecular breeding in H. marmoreus. A total of 2000 proteins were classified into eight different modules by WGCNA, wherein 490 proteins were classified into the turquoise module. (4) Conclusions: Generally, from the 3rd day to the 10th day after scratching, mycelium recovered gradually and formed primordia. Importin, dehydrogenase, heat-shock proteins, ribosomal proteins, transferases were all highly expressed in these three developmental stages. DEPs in the Rec stage compared with the Knot or Pri stages were significantly enriched in the metabolic-, catabolic- and carbohydrate-related process; and in oxidoreductase, peptidase and hydrolase activities. This research contributes to the understanding of the mechanisms of the development changes before primordium of H. marmoreus.

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UDP-glucose pyrophosphorylase gene affects mycelia growth and polysaccharide synthesis of Grifola frondosa

Cited by 23 publications

References 35 publications

Enhancement of polysaccharides production using microparticle enhanced technology by Paraisaria dubia

Enhancement of polysaccharides production using microparticle enhanced technology by Paraisaria dubia

Transcription factor GlbHLH regulates hyphal growth, stress resistance, and polysaccharide biosynthesis in Ganoderma lucidum

Comparative Proteomic Analyses within Three Developmental Stages of the Mushroom White Hypsizygus marmoreus

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