UDP-glucosyltransferase PpUGT85A2 controls volatile glycosylation in peach

Abstract

Synthesis of the major glycosylated monoterpene linalool in peach is catalyzed by PpUGT85A2.

Glycosylation of bioactive C13-apocarotenols inNicotiana benthamianaandMentha × piperita

“…In comparison, UGT75L6 from G. jasminoides, which forms a glucose ester showed an apparent K M value for the C 20 -apocarotenoid crocetin of 0.46 mM (Nagatoshi et al 2012), while the apparent K M value for the C 10apocarotenoid 3-hydroxy-β-cyclocitral of UGT709G1 from C. sativus was 64.0 µM (Diretto et al 2019). UGTs acting on terpenes show K M values from 9-463 µM for monoterpenols such as citronellol, geraniol, nerol and linalool and kcat/K M values from 40-2600 M -1 sec -1 for the same substrates (Bönisch et al 2014;Wu et al 2019).…”

“…UGT85A73 is probably implicated in the glucosylation of tobacco flower volatiles as it is strongly expressed in tobacco blossom and glucosylates nonpolar, low-molecularweight compounds (Caputi et al 2012;Sun et al 2019). UGT85s from peach, grape, tea plant, and kiwifruit catalyze the glucosylation of aliphatic alcohols including linalool, geraniol, citronellol, hexanol, (Z)-3-hexenol, octanol and also volatiles such as 2-phenylethanol, benzyl alcohol, and furaneol (Bönisch et al 2014;Jing et al 2019;Song et al 2018;Wu et al 2019).…”

Transient Gene Expression is an Effective Experimental Tool for the Research into the Fine Mechanisms of Plant Gene Function: Advantages, Limitations, and Solutions

“…The results of predictions give information not only about putative localization of the protein product, but also, and frequently, about the likely location of signal peptides (in the N- or C-terminal region), which can mediate the specific localization of the target protein. These predictions are used to design the gene constructs for experimental verification of the protein localization in the predicted compartments taking into account the information about localization of the signal peptide in the protein sequence (in the N- or C-terminal region) [ 38 , 42 , 43 ]. The gene constructs for experimental verification carry the hybrid gene where the target gene is transcriptionally and translationally fused with the gene coding for, as a rule, a fluorescent protein under the control of a strong constitutive promoter ( Figure 2 part 1 or 2).…”

Transcriptome co-expression network analysis identifies key genes and regulators of ripening kiwifruit ester biosynthesis

“…Measurement of postharvest kiwifruit volatiles and analysis were carried out according to Zhang et al and Wu et al [12,39]. Frozen flesh tissue was ground in liquid nitrogen and a total of 2 g flesh transferred to a 10 mL vial containing 3 mL saturated sodium chloride solution.…”