UDPglucosyltransferase and Its Kinetic Fluorimetric Assay

Väisänen, Matti; Mackenzie, Peter I.; Hänninen, Osmo

doi:10.1111/j.1432-1033.1983.tb07128.x

Cited by 8 publications

(2 citation statements)

References 17 publications

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“…Väisänen et al (1983) reported significant activation of the enzyme's activity by Mg 2+ . Later, Elmamlouk and Gessner (1978) examined the UDPG transferases in the hepatopancreas of the lobster Homarus americanus and found that among several carbohydrates, only glucose is transferred from the corresponding UDP to p-nitrophenol (NP) at an optimal pH of about 8.…”

Section: Glucose and Glucuronic Acid Transferasesmentioning

confidence: 96%

“…has insignificant UDPG transferase activity (Yang et al 2002). The enzyme activity of some crustaceans is localized in the microsomal fraction of hepatopancreas or green glands (Elmamlouk and Gessner 1978;Hänninen et al 1984;Li and James 1993;Schell and James 1989;Väisänen et al 1983). Exposure of D. magna to diethylstilbestrol enhanced the formation of glucose conjugates of testosterone, but its presence was found to also slightly inhibit enzyme activity (Baldwin et al 1995).…”

Section: Glucose and Glucuronic Acid Transferasesmentioning

confidence: 98%

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Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in Aquatic Organisms

Katagi

2009

Reviews of Environmental Contamination and Toxicology

137

102

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The ecotoxicological assessment of pesticide effects in the aquatic environment should normally be based on a deep knowledge of not only the concentration of pesticides and metabolites found but also on the influence of key abiotic and biotic processes that effect rates of dissipation. Although the bioconcentration and bioaccumulation potentials of pesticides in aquatic organisms are conveniently estimated from their hydrophobicity (represented by log K(ow), it is still indispensable to factor in the effects of key abiotic and biotic processes on such pesticides to gain a more precise understanding of how they may have in the natural environment. Relying only on pesticide hydrophobicity may produce an erroneous environmental impact assessment. Several factors affect rates of pesticide dissipation and accumulation in the aquatic environment. Such factors include the amount and type of sediment present in the water and type of diet available to water-dwelling organisms. The particular physiological behavior profiles of aquatic organisms in water, such as capacity for uptake, metabolism, and elimination, are also compelling factors, as is the chemistry of the water. When evaluating pesticide uptake and bioconcentration processes, it is important to know the amount and nature of bottom sediments present and the propensity that the stuffed aquatic organisms have to absorb and process xenobiotics. Extremely hydrophobic pesticides such as the organochlorines and pyrethroids are susceptible to adsorb strongly to dissolved organic matter associated with bottom sediment. Such absorption reduces the bioavailable fraction of pesticide dissolved in the water column and reduces the probable ecotoxicological impact on aquatic organisms living the water. In contrast, sediment dweller may suffer from higher levels of direct exposure to a pesticide, unless it is rapidly degraded in sediment. Metabolism is important to bioconcentration and bioaccumulation processes, as is detoxification and bioactivation. Hydrophobic pesticides that are expected to be highly stored in tissues would not be bioconcentrated if susceptible to biotic transformation by aquatic organisms to more rapidly metabolized to hydrophilic entities are generally less toxic. By analogy, pesticides that are metabolized to similar entities by aquatic species surely are les ecotoxicologically significant. One feature of fish and other aquatic species that makes them more relevant as targets of environmental studies and of regulation is that they may not only become contaminated by pesticides or other chemicals, but that they constitute and important part of the human diet. In this chapter, we provide an overview of the enzymes that are capable of metabolizing or otherwise assisting in the removal of xenobiotics from aquatic species. Many studies have been performed on the enzymes that are responsible for metabolizing xenobiotics. In addition to the use of conventional biochemical methods, such studies on enzymes are increasingly being conducted using immunochemical met...

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Section: Glucose and Glucuronic Acid Transferasesmentioning

confidence: 96%

Section: Glucose and Glucuronic Acid Transferasesmentioning

confidence: 98%

Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in Aquatic Organisms

Katagi

2009

Reviews of Environmental Contamination and Toxicology

137

102

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Separation by FPLC chromatofocusing of UDP-glucosyltransferases from three developmental stages ofDrosophila melanogaster

Rausell

Llorca

Real

1997

Arch. Insect Biochem. Physiol.

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Variation of UDP‐glucosyltransferase activity, during Drosophila melanogaster development, was analyzed. The endogenous metabolite xanthurenic acid and the xenobiotic compounds 1‐naphthol and 2‐naphthol were used as substrates. Developmentally regulated differences were observed for the three substrates, suggesting the presence of UDP‐glucosyltransferase isoenzymes. This was further confirmed by FPLC chromatofocusing on a Mono P column: seven peaks of UDP‐glucosyltransferase activity (pHs: ≥6.3, 5.8, 5.5, 4.9, 4.5, 4.2, ≤4.0) with either single or overlapping substrate specificity were detected. A single xanthurenic acid:UDP‐glucosyltransferase activity (pl 5.8) was found throughout development. In contrast, a gradual increase in the number of 2‐napthol:UDP‐glucosyltransferase isoenzymes (pl from 6.3 to 4.0) was observed during development, whereas no isoenzymes specific for 1‐naphthol were resolved. Based on the distribution and substrate specificity of the eluted peaks in the three developmental stages analyzed, the presence of seven or possibly eight UDP‐glucosyltransferase isoenzymes is proposed. Arch. Insect Biochem. Physiol. 34:347–358, 1997. © 1997 Wiley‐Liss, Inc.

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UDP-glucosyltransferase activity toward exogenous substrates in Drosophila melanogaster

Real

Ferré

Chapa

1991

Analytical Biochemistry

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UDPglucosyltransferase and Its Kinetic Fluorimetric Assay

Cited by 8 publications

References 17 publications

Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in Aquatic Organisms

Bioconcentration, Bioaccumulation, and Metabolism of Pesticides in Aquatic Organisms

Separation by FPLC chromatofocusing of UDP-glucosyltransferases from three developmental stages ofDrosophila melanogaster

UDP-glucosyltransferase activity toward exogenous substrates in Drosophila melanogaster

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