Ueber das Thymin, ein Spaltungsproduct der Nucleïnsäure

The occurrence and distribution of thymine and three methylated-adenine bases in ribonucleic acids from several sources

“…The present work has not only confirmed this finding but has indicated that three methylated adenine bases, 2-methyladenine, 6methylaminopurine and 6-dinethylaminopurine, as well as thymine, are naturally present in small amounts in ribonucleic acid from a variety of sources. None of these compounds has been described previously as a component of ribonucleic acid, but all four are known to occur naturally as nucleosides or nucleotides, since thymine and 6methylaminopurine occur in deoxyribonucleic acid (Kossel & Neumann, 1893;Dunn & Smith, 1958a), 2-methyladenine in the vitamin B12-like Factor A extracted from E. coli and other sources (Dion, Calkins & Pfiffner, 1954;Brown, Cain, Gant, Parker & Smith, 1955) and 6-dimethylaminopurine in puromycin formed by the actinomycete Streptomyces alboniger (Waller, Fryth, Hutchings & Williams, 1953). The presence of 6-methylaminopurine in ribonucleic acid from yeast has also been reported by Adler, Weissmann & Gutman (1958).…”

Thymidylate Synthetase

“…The overall reaction (eq. 1) catalyzed by thymidylate synthetase [EC 2.1.1.61 is usually written as ~s,"o-methylene-H~-folate + dUMP + dTMP + H2-folate (1) The purpose of this review is to consider the mechanism of action of thymidylate synthetase as well as genetic and regulatory aspects that influence its activity. Finally, thymidylate synthetase, a target in cancer chemotherapy, is discussed with special emphasis on chemical antagonists that have been employed as inhibitors of this enzyme.…”

Overview of Methods for Large-Scale RNA Synthesis

“…In early nucleic acid research, there was no clear distinction between RNA and deoxyribonucleic acid (DNA); they were seen as "nuclein", a phosphor-rich substance different from proteins and present in large quantities in every living organism [4]. The pioneering work of many researchers, including Phoebus Levene, Albrech Kossel, and their co-workers, allowed to distinguish DNA and RNA molecules and to identify their basic components, such as ribose rings, nitrogen-containing bases, and phosphate groups [5][6][7]. Since then, RNA has been revealed as a multifaceted molecule that can form complex three-dimensional structures, presents catalytic properties, and serves as a regulatory element for governing gene expression [8][9][10][11][12][13][14][15][16].…”