UHPLC-ESI-MS/MS assay for quantification of endocannabinoids in cerebrospinal fluid using surrogate calibrant and surrogate matrix approaches

Aydin, Ece; Cebo, Malgorzata; Mielnik, Justyna; Richter, Hardy; Schüle, Rebecca; Sievers-Engler, Adrian; Młynarz, Piotr; Lämmerhofer, Michael

doi:10.1016/j.jpba.2022.115090

Cited by 10 publications

(4 citation statements)

References 32 publications

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“…The variability of the matrix interference results was within 20% RSD for all the NAEs. A study by Aydin et al (26) reported slight ion enhancement using a CSF surrogate which was not the case in our case with human CSF samples.…”

Section: Resultscontrasting

confidence: 74%

“…The LOQ of this method for OEA-d 4 (0.374 ng mL -1 ) was lower in comparison to a previous approach using a similar method (3.62 ng mL -1 ) [24]. The AEA LOQ of 1.07 ng mL -1 in human CSF was reported in another study [1] using a micro-flow LC-MS/MS approach.…”

Section: Resultsmentioning

confidence: 63%

“…A study by Aydin et al [24] reported slight ion enhancement in the matrix effect. They have used surrogate CSF which may have resulted in increased ionization efficiency of eCBs due to the presence of another compound.…”

Section: Resultsmentioning

confidence: 99%

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A UHPLC–MS/MS method for determination of N-acylethanolamines in cerebrospinal fluid

Ismoilova,

Wretlind,

Hooshmand

et al. 2023

Preprint

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Endocannabinoids (eCBs) are endogenous lipids that activate cannabinoid receptors. Their role in neuroinflammation is of a great interest due to the range of physiological effects they exhibit, potentially serving as bioindicators of pathologies. However, they are present at nanomolar concentrations in biological samples, posing challenges for detection and quantification. A method for quantitative analysis of eight exogenous eCB internal standards (anandamide-d4, docosahexaenoyl ethanolamide-d4, eicosapentaenoyl ethanolamide-d4, linoleoyl ethanolamide-d4, oleoyl ethanolamide-d4, palmitoyl ethanolamide-d5, stearoyl ethanolamide-d3 and palmitanilide) in cerebrospinal fluid is reported here. Six monophasic solvents (methanol, acetonitrile, chloroform/methanol/water, acetonitrile/acetone, isopropanol/acetonitrile/water, and ethanol) were compared in their extraction efficiency. Detection of analytes was performed using an ultra-high performance liquid chromatography method coupled to tandem mass spectrometry (UHPLC-MS/MS) in dynamic multiple reaction monitoring (dMRM) mode. The method was further optimised based on accuracy, precision, matrix effect, linearity, limits of detection and quantification. The method was applied to a cohort of healthy individuals (n=33) to identify and estimate the concentration of eCBs in CSF.

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Section: Resultscontrasting

confidence: 74%

Section: Resultsmentioning

confidence: 63%

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A UHPLC–MS/MS method for determination of N-acylethanolamines in cerebrospinal fluid

Ismoilova,

Wretlind,

Hooshmand

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…If the RF is not stable and SILs are used as surrogate calibrants, the analyst should investigate the potential presence of crosstalk interferences such as isotopic pattern overlap or impurities coming from SIL standards [116,117]. The RF can diverge from unit value when SILs containing enriched hydrogen atoms are used, but as long as the unit value of slope parallelism is within 15% acceptance the concerned SILs can be selected as surrogate calibrants [118]. For example, tryptophan was successfully quantified in plasma with a relative bias between − 2.0 and − 8.0 using its deuterated analog, even if the response factor was 0.67 [119].…”

Section: Surrogate Analyte(s) In Authentic Matrixmentioning

confidence: 99%

From fundamentals in calibration to modern methodologies: A tutorial for small molecules quantification in liquid chromatography–mass spectrometry bioanalysis

Visconti

Bernard

Feinberg

et al. 2023

Analytica Chimica Acta

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Advances in targeted liquid chromatography‐tandem mass spectrometry methods for endocannabinoid and N‐acylethanolamine quantification in biological matrices: A systematic review

Amir Hamzah,

Turner,

Nichols

et al. 2024

Mass Spectrometry Reviews

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Liquid chromatography paired with tandem mass spectrometry (LC‐MS/MS) is the gold standard in measurement of endocannabinoid concentrations in biomatrices. We conducted a systematic review of literature to identify advances in targeted LC‐MS/MS methods in the period 2017–2024. We found that LC‐MS/MS methods for endocannabinoid quantification are relatively consistent both across time and across biomatrices. Recent advances have primarily been in three areas: (1) sample preparation techniques, specific to the chosen biomatrix; (2) the range of biomatrices tested, recently favoring blood matrices; and (3) the breadth of endocannabinoid and endocannabinoid‐like analytes incorporated into assays. This review provides a summary of the recent literature and a guide for researchers looking to establish the best methods for quantifying endocannabinoids in a range of biomatrices.

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UHPLC-ESI-MS/MS assay for quantification of endocannabinoids in cerebrospinal fluid using surrogate calibrant and surrogate matrix approaches

Cited by 10 publications

References 32 publications

A UHPLC–MS/MS method for determination of N-acylethanolamines in cerebrospinal fluid

A UHPLC–MS/MS method for determination of N-acylethanolamines in cerebrospinal fluid

From fundamentals in calibration to modern methodologies: A tutorial for small molecules quantification in liquid chromatography–mass spectrometry bioanalysis

Advances in targeted liquid chromatography‐tandem mass spectrometry methods for endocannabinoid and N‐acylethanolamine quantification in biological matrices: A systematic review

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