UHPLC‐MS Analyses of Plant Flavonoids

Lei, Zhentian; Sumner, Barbara W.; Bhatia, Ashok Kumar; Sarma, Saurav J.; Sumner, Lloyd W.

doi:10.1002/cppb.20085

Cited by 28 publications

(22 citation statements)

References 17 publications

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“…Flavonoids biosynthesis pathway has been extensively studied 51 , 52 . In G. biloba , several transcription factors were cloned and identified to be involved in biosynthetic pathway of flavonoids.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and characterization of bHLH family genes from Ginkgo biloba

Zhou

Liao

Kim

et al. 2020

Sci Rep

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Basic helix–loop–helix (bHLH) proteins, one of the most important and largest transcription factor family in plants, play important roles in regulating growth and development, stress response. In recent years, many bHLH family genes have been identified and characterized in woody plants. However, a systematic analysis of the bHLH gene family has not been reported in Ginkgo biloba , the oldest relic plant species. In this study, we identifed a total of 85 GbbHLH genes from the genomic and transcriptomic databases of G. biloba , which were classified into 17 subfamilies based on the phylogenetic analysis. Gene structures analysis indicated that the number of exon–intron range in GbbHLHs from 0 to 12. The MEME analysis showed that two conserved motifs, motif 1 and motif 2, distributed in most GbbHLH protein. Subcellular localization analysis exhibited that most GbbHLHs located in nucleus and a few GbbHLHs were distributed in chloroplast, plasma membrane and peroxisome. Promoter cis-element analysis revealed that most of the GbbHLH genes contained abundant cis-elements that involved in plant growth and development, secondary metabolism biosynthesis, various abiotic stresses response. In addition, correlation analysis between gene expression and flavonoid content screened seven candidate GbbHLH genes involved in flavonoid biosynthesis, providing the targeted gene encoding transcript factor for increase the flavonoid production through genetic engineering in G. biloba .

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Section: Discussionmentioning

confidence: 99%

Genome-wide identification and characterization of bHLH family genes from Ginkgo biloba

Zhou

Liao

Kim

et al. 2020

Sci Rep

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“…Moreover, flavonoids play an important role in various plant physiological or developmental functions, as well as in the agronomic and industrial qualities of plant products ( Lepiniec et al, 2006 ; Li et al, 2018 ). Numerous studies have found that flavonoids have antioxidant, anti-inflammatory, antimicrobial, anti-fungal, antiviral, anti-tumor properties, and also help to scavenge free radicals and stabilize the reactive oxygen species; these keep the body safe from chronic diseases, such as cancer, diabetes, and cardiovascular and heart disease, enhance the immune system, for example, suppress pro-inflammatory immune response, in particular auto-reactive T cells ( Havsteen, 2002 ; Verbeek et al, 2004 ; Falcone Ferreyra et al, 2012 ; Mouradov and Spangenberg, 2014 ; Lei et al, 2019 ; Sarbu et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Variations in Flavonoid Metabolites Along Altitudinal Gradient in a Desert Medicinal Plant Agriophyllum squarrosum

et al. 2021

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Agriophyllum squarrosum (L.) Moq., a pioneer plant endemic to the temperate deserts of Asia, could be domesticated into an ideal crop with outstanding ecological and medicinal characteristics. A previous study showed differential flavonoid accumulation between two in situ altitudinal ecotypes. To verify whether this accumulation was determined by environmental or genetic factors, we conducted flavonoid-targeted metabolic profiling among 14 populations of A. squarrosum collected from regions with different altitudes based on a common garden experiment. Results showed that the most abundant flavonoid in A. squarrosum was isorhamnetin (48.40%, 557.45 μg/g), followed by quercetin (13.04%, 150.15 μg/g), tricin (11.17%, 128.70 μg/g), isoquercitrin (7.59%, 87.42 μg/g), isovitexin (7.20%, 82.94 μg/g), and rutin (7.00%, 80.62 μg/g). However, based on a common garden at middle-altitude environment, almost none of the flavonoids was enriched in the high-altitude populations, and even some flavonoids, such as quercetin, tricin, and rutin, were significantly enriched in low-altitude populations. This phenomenon indicated that the accumulation of flavonoids was not a result of local adaptation to high altitude. Furthermore, association analysis with in situ environmental variables showed that the contents of quercetin, tricin, and rutin were strongly positively correlated with latitude, longitude, and precipitation gradients and negatively correlated with temperature gradients. Thus, we could conclude that the accumulations of flavonoids in A. squarrosum were more likely as a result of local adaption to environmental heterogeneity combined with precipitation and temperature other than high altitude. This study not only provides an example to understand the molecular ecological basis of pharmacognosy, but also supplies methodologies for developing a new industrial crop with ecological and agricultural importance.

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“…In the vegetable kingdom, flavonoids are reported to play a significant role in plant resistance, both in terms of abiotic and biotic stresses (Nayak et al, 2015), such as drought, UV radiation, and heavy metal contamination (Izaguirre et al, 2007;Lei et al, 2019). In addition to their function in abiotic stress resistance, flavonoids have also been found to be involved in plant protection.…”

Section: Introductionmentioning

confidence: 99%

AtMYB12-Expressing Transgenic Tobacco Increases Resistance to Several Phytopathogens and Aphids

Ding

Zhang

et al. 2021

Front. Agron.

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Phytopathogens and pests are two major factors that limit the growth of plants. The expression of a flavonoid regulator gene, AtMYB12(AT2G47460), has been reported to increase the endogenous flavonoid content of tobacco and tomato. Previous research has only focused on the regulation mechanism of v-myb avian myeloblastosis viral oncogene homolog (MYB) transcription factors under single stress conditions. Here, research showed that AtMYB12 was involved in regulating the resistance of tobacco to multiple biological stresses such as phytopathogens and aphid. We reported that transgenic tobacco carrying AtMYB12 was more resistant to Ralstonia solanacearum when the up-regulated expression of several defense-related markers, such as NbPR1a, NbNOA1, and NbrbohB, was activated, suggesting that the priming defense of a plant may contribute to bacterial disease resistance. The improvement of the resistance of AtMYB12-expressing transgenic tobacco is achieved by promoting the production of ROS, H2O2, and NO. AtMYB12-expressing transgenic tobacco also has resistance to fungal pathogens, such as Colletotrichum nicotianae Averna and Alternaria alternate. The enrichment of flavonols components, such as rutin, which directly inhibit the growth of C. nicotianae and A. alternate, may also contribute to the defense mediated by AtMYB12 over-expression. At the same time, the results also confirm that AtMYB12-expressing transgenic tobacco enhanced plant resistance to aphid-infested (Aphidoidea) pests. These results suggest that the AtMYB12 gene is a good candidate for pest and disease control, with limited resistance costs and enrichment in flavonols, and that AtMYB12 has a potential in the breeding of disease-resistant tobacco crops.

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UHPLC‐MS Analyses of Plant Flavonoids

Cited by 28 publications

References 17 publications

Genome-wide identification and characterization of bHLH family genes from Ginkgo biloba

Genome-wide identification and characterization of bHLH family genes from Ginkgo biloba

Variations in Flavonoid Metabolites Along Altitudinal Gradient in a Desert Medicinal Plant Agriophyllum squarrosum

AtMYB12-Expressing Transgenic Tobacco Increases Resistance to Several Phytopathogens and Aphids

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