UHPLC-MS for the analytical characterization of traditional Chinese medicines

Wang, Xijun; Zhang, Aihua; Ge, Yan; Han, Ying; Sun, Hui

doi:10.1016/j.trac.2014.05.013

Cited by 89 publications

(50 citation statements)

References 120 publications

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“…Consistent with the holistic concept of TCM, the integrated strategy of metabolomics has been well accepted in TCM studies Chen et al, 2016;Kim et al, 2016). Recently, more and more studies applied metabolomics approaches for chemical characterization, bioactive component screening, efficacy evaluation of TCM herbs or formulas (Hu et al, 2014;Wang et al, 2014;Shi et al, 2015). For example, UPLC-QTOF-MS/MS-based metabolomics approach had been used for evaluating the holistic qualities and exploring the characteristic chemical components of commercial white ginseng (WG) and red ginseng (RG).…”

Section: Discussionmentioning

confidence: 99%

Differences in Chemical Component and Anticancer Activity of Green and Ripe Forsythiae Fructus

et al. 2017

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Forsythiae Fructus, Lianqiao in Chinese, is one of the most fundamental herbs in Traditional Chinese Medicine. Both green Forsythia (GF) and ripe Forsythia (RF) are referred to Forsythiae Fructus in medicinal applications. In most cases, they are used without distinction. In this study, a metabolomics approach was performed to compare componential differences of two Forsythiae Fructus aqueous extracts subtypes. Principal component analysis (PCA) score plots from the UPLC-MS data showed clear separation between the two subtypes, indicating there are significant differences in the chemical components between GF and RF. Meanwhile, the anticancer activity of them was also compared. GF exhibited much stronger antitumor activity than RF against B16-F10 murine melanoma both in vitro and in vivo. 15 chemical compounds were identified as specific markers for distinguishing GF and RF. Among these marker compounds, forsythoside I, forsythoside A, forsythoside E and pinoresinol were demonstrated to be key important active compounds that account for the different anticancer efficacies of GF and RF. Our data suggest that GF and RF should be distinctively used in clinical applications, particularly in the anticancer formulas, in which GF should be preferentially prescribed.

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Section: Discussionmentioning

confidence: 99%

Differences in Chemical Component and Anticancer Activity of Green and Ripe Forsythiae Fructus

et al. 2017

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“…UHPLC-MS is gradually accepted and applied to TCMs. In 2014, Wang et al [88] published a comprehensive review on the applications of UHPLC-MS in TCM research, highlighting its advantages with characteristic examples. In this section, we tend to give an overview of application UHPLC in TCM separation, focusing on high-efficiency stationary phases (Table 1).…”

Section: High-efficiency Stationary Phases For Tcmsmentioning

confidence: 99%

Recent development in liquid chromatography stationary phases for separation of Traditional Chinese Medicine components

Jin

Liu

Guo

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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Traditional Chinese Medicine (TCM) is an ancient medical practice which has been used to prevent and cure diseases for thousands of years. TCMs are frequently multi-component systems with mainly unidentified constituents. The study of the chemical compositions of TCMs remains a hotspot of research. Different strategies have been developed to manage the significant complexity of TCMs, in an attempt to determine their constituents. Reversed-phase liquid chromatography (RPLC) is still the method of choice for the separation of TCMs, but has many problems related to limited selectivity. Recently, enormous efforts have been concentrated on the development of efficient liquid chromatography (LC) methods for TCMs, based on selective stationary phases. This can improve the resolution and peak capacity considerably. In addition, high-efficiency stationary phases have been applied in the analysis of TCMs since the invention of ultra high-performance liquid chromatography (UHPLC). This review describes the advances in LC methods in TCM research from 2010 to date, and focuses on novel stationary phases. Their potential in the separation of TCMs using relevant applications is also demonstrated.

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“…Pharmaceutical analysis, as an important approach to understand the active pharmaceutical ingredients and impurities, to ensure the efficacy and safety, as well as to develop methodologies for quality assessment and control of pharmaceutical products and herbal medicines, has been a hot research topic in recent years. Huge efforts have been spent in this field, with different techniques including optical spectroscopy [5][6][7], chromatography [7][8][9][10][11][12][13][14], and mass spectrometry (MS) [12][13][14][15][16][17][18][19][20], etc., have been developed in the past several decades. The chromatography and chromatography coupled with MS techniques have been proven to be the powerful tools due to their properties of excellent sensitivity, desirable specificity, and reasonable qualitative and quantitative capability.…”

Section: Introductionmentioning

confidence: 99%

Analysis of pharmaceutical products and herbal medicines using ambient mass spectrometry

Yang

Deng

2016

TrAC Trends in Analytical Chemistry

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UHPLC-MS for the analytical characterization of traditional Chinese medicines

Cited by 89 publications

References 120 publications

Differences in Chemical Component and Anticancer Activity of Green and Ripe Forsythiae Fructus

Differences in Chemical Component and Anticancer Activity of Green and Ripe Forsythiae Fructus

Recent development in liquid chromatography stationary phases for separation of Traditional Chinese Medicine components

Analysis of pharmaceutical products and herbal medicines using ambient mass spectrometry

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