UHPLC-MS/MS method for simultaneous determination of Radix Polygalae glycolipids and organic acids in rat plasma and application in a pharmacokinetic study

Protective Effects and Mechanism of Radix Polygalae Against Neurological Diseases as Well as Effective Substance

“…The components of RP include saponins, oligosaccharides, ketones, alkaloids, polysaccharides, and flavonoids, and it has pharmacological effects related to sedation, anticonvulsive action, antidepressant action, anti-myocardial ischemic action, and learning and memory enhancement, as shown in Figure 1. Due to its neuroprotective effects on nerve cells, such as antidepressive effects and learning and memory improvements (12,13), these extracts and active ingredients are receiving increasing attention from researchers.…”

Ácido 3,4,5-Trimetoxicinâmico e seu Potencial Terapêutico: Uma Revisão / 3,4,5-Trimethoxycinnamic Acid and Its Therapeutic Potential: A Review

“…A ultra cromatografia líquida de alta eficiência acoplada a espectrometria de massa sequencial (UHPLC-MS/MS) é uma técnica que utiliza menores volumes de amostras e proporciona análises mais rápidas e com melhor detectabilidade (Oshita et al, 2015). Sendo assim, foi desenvolvido um método de UHPLC-MS/MS para determinação simultânea de sete componentes ativos do extrato das raízes de Polygala tenuifolia Wild, no qual um destes era o TMCA (Xu et al, 2018). Os analitos foram detectados por MRM e a validação do método mostrou boa linearidade, exatidão, precisão e estabilidade das amostras.…”

“…Um estudo analisou o perfil farmacocinético do TMCA em plasmas de ratos após a administração do extrato das raízes de Polygala tenuifolia Wild através do método UHPLC-MS/MS (Xu et al, 2018). É possível observar, a partir deste estudo, que o TMCA foi rapidamente absorvido após a administração oral, devido ao seu baixo peso…”

UHPLC-MS/MS method for pharmacokinetic and bioavailability determination of five bioactive components in raw and various processed products of Polygala tenuifolia in rat plasma

“…Moreover, the pharmacokinetics of TFSA, tenuifoliside C, polygalaxanthone III and tenuifolin from PT extract in rat plasma were acquired using LC-MS/MS (Lin et al 2014b). Recently, our research group established a UHPLC-MS/MS method for the simultaneous and quantitative determination of A5, A6, DSS, TFSA, tenuifoliside B, tenuifoliside C and TMCA in rat plasma (Xu et al 2018b). However, pharmacokinetic comparisons of the main active components among raw and various processed products of PT have not been reported.…”

“…Recently, our research group established a UHPLC-MS/MS method for the simultaneous and quantitative determination of A5, A6, DSS, TFSA, tenuifoliside B, tenuifoliside C and TMCA in rat plasma (Xu et al. 2018b ). However, pharmacokinetic comparisons of the main active components among raw and various processed products of PT have not been reported.…”