UHPLC-MS/MS phytochemical screening, polyphenolic content and antioxidant potential of Diplorhynchus condylocarpon (Müll.Arg.) Pichon (Apocynaceae), a medicinal plant

Bitwell, Chibuye; Sen, Singh Indra; Chimuka, Luke; Kakoma, Maseka Kenneth

doi:10.1016/j.sciaf.2023.e01712

Cited by 6 publications

(4 citation statements)

References 40 publications

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“…Firstly, our decision to use only the entire plant of O. foetida may have obscured the potential differences and richness in phenolic compounds across different plant parts (stem, flowers, and seeds …) as reported in previous studies. ( Khanam et al, 2015 ; Bitwell et al, 2023 ). Secondly, Expanding the sample size (n = 6) in our in vivo study could enhance the statistical power and address any ambiguity regarding parameter variations.…”

Section: Discussionmentioning

confidence: 99%

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Unlocking the hepatoprotective potential of the parasitic plant Orobanche foetida Poir. aqueous extract against CCl4-induced liver injury in rat

Bedoui,

Mufti,

Feriani

et al. 2024

Front. Pharmacol.

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In this current study, we explored the preventive capacity of the aqueous extract of Orobanche foetida (OF), a root holoparasite, against CCl4 prompt hepatotoxicity in rats. LC-MS/MS profiling revealed the existence of 32 compounds belonging to organic acids, benzoic acid derivatives, and hydroxycinnamic acids along with their glycosides and derivatives as well as several flavonoids. In vitro, OF demonstrated substantial antioxidant potential at DPPH and ABTS assays. Results showed that the pretreatment with OF for 6 weeks at the doses (25 mg/kg bw) and (50 mg/kg bw) countered the CCl4-induced liver injury by restoring liver injuries indicators (ALT, AST, LDH, ALP, GGT and bilirubin), normalizing lipid profile (TC, TG, LDL-C, and HDL-C), as well as, impeding DNA fragmentation. Furthermore, OF blocked the hepatic oxidative stress spurred by CCl4 administration through boosting antioxidant enzymes (GSH, CAT, and SOD) responsible of diminishing lipid peroxidation. exhibited an anti-inflammatory effect by downregulating TNF-α and IL-6 levels. OF suppressive effect on proinflammatory cytokines is further exerted by its capacity to modulate the expression of the NF-κB gene. In silico investigation revealed that among the 32 identified compounds, vanillic acid glucoside and dihydroxybenzoic acid glucoside have strong and stable bindings with the active sites of three key inflammatory proteins (PARP-1, TNF-α, IL-6), which could highlight the antioxidant and anti-inflammatory capacity of. Overall, this research provides a preliminary pharmacological support for the medicinal applications of Orobanche foetida for addressing inflammatory and hepato-pathological conditions.

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Section: Discussionmentioning

confidence: 99%

“…as reported in previous studies. (Khanam et al, 2015;Bitwell et al, 2023). Secondly, Expanding the sample size (n = 6) in our in vivo study could enhance the statistical power and address any ambiguity regarding parameter variations.…”

Section: Conventional Hydrogen Bondmentioning

confidence: 99%

Unlocking the hepatoprotective potential of the parasitic plant Orobanche foetida Poir. aqueous extract against CCl4-induced liver injury in rat

Bedoui,

Mufti,

Feriani

et al. 2024

Front. Pharmacol.

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“…Secondary metabolites obtained from the plants are considered important as source for development of innovative therapeutic agents and researchers from all over the world are using medicinal plants for exploring new bioactive compounds [12]. In the current research work, the exploration of phytochemicals in ethanolic extract of Ficus carica L. leaves by means of chemical tests have shown the presence of many important metabolites that include tannins, phenols, glycosides, flavonoids, resins, alkaloids, steroids and terpenoids.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical and Biological Investigations of <em>Ficus carica</em> L. Leaf Extract Along with Formulation Development

Hasnain,

Ahmed,

Alzahrani

et al. 2023

Preprint

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Utilization of medicine derived from plants has been documented in various traditional systems of medicines throughout the world for different diseases. Therefore, the current research work was carried out to evaluate the phytochemical and biological potential of Ficus carica L ethanolic leaf extract. For this purpose, physicochemical and phytochemical analysis, total phenolic and flavonoids contents was performed. Antioxidant potential was determined by FRAP, DPPH and H2O2 assay. Proteinase inhibition, heat induced hemolysis and BSA denaturation assay was carried out for inflammation. Antiglycation potential was assessed by fructosamine assay, Congo-red assay and estimation of free carbonyl groups. In-vivo antidiabetic, anti-obesity, effect on liver and kidney were investigated by high fat high sugar diet model. FTIR, HPLC and LCMS/MS analysis was performed to find out the compounds. Tablet based formulation was developed using wet granulation method and tested for physicochemical parameters. Results shows that total phenolic and flavonoids content was (333 mg GAE/g) and (123 mg RE/g) respectively, H2O2 assay shows 35.6% free radical inhibition potential, DPPH assay shows (IC50) inhibitory concentration at 0.58 mg/mL, and 88.769 µg/g Fe2SO4 solution FRAP values. Proteinase inhibition assay, Heat induced hemolysis and BSA-denaturation assay shows percentage inhibition of 28 ± 0.01, 55 ± 0.03 and 51.2 ± 0.05 respectively. In Antiglycation activity β-amyloid formation assay shows absorption of 0.017, Fructosamine assay shows inhibition of 19.4±0.06 and free carbonyl group estimation shows inhibition of 17.0±0.03. In in-vivo study FLE at (500 mg/kg) significantly decreased total bilirubin (p ≤ 0.001), decreased (p ≤ 0.05) alanine transferase, aspartate aminotransferase, alkaline phosphatase, decreased blood glucose, triglycerides and total cholesterol (p ≤ 0.01) while HDL (p ≤ 0.01) was significantly increased in comparison to obesity and diabetic-obesity groups. Different functional groups presence was confirmed by FTIR spectrum. HPLC analysis revealed the existence of ferulic acid, rutin, chlorogenic acid, coumarin and thymoquinone and these results are corelated by LC-MS/MS. Pre-compression characterization showed angle of repose (33.04°), bulk density (0.54 g/cm3), tapped density (0.73 g/cm3), compressibility index (35.179 %) and Hausner’s ratio (1.35), while the post-compression characterization showed weight variation 826±4.17 mg, thickness 10.50±0.25 kg/cm2, friability 0.57 %, and disintegration time 8.19 min. From the above results it is concluded that F. carica L. leaves extract has strong antioxidant, antidiabetic and hypolipidemic potential and can be used for an obesity and diabetes treatment.

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“…Bioactive secondary metabolites that have pharmacological and toxicological effects on humans such as alkaloids, polyphenols, tannins, flavonoids, steroids, terpenoids, and many others. These bioactive compounds are found in various parts of plants, such as flowers, seeds, stems, bark, fruit, leaves and roots (Bitwell et al, 2023). Apart from plants, bioactive compounds are also found in animals, microbes and other marine organisms (Puspitasari, 2018).…”

Section: Introductionmentioning

confidence: 99%

Subchronic Toxicity Test of Purple Leaves Ethanol Extract (PLEE) on the Histopathological Picture of the Pancreas of Wistar Rats

Wardani,

Dewi,

Alaydrus

et al. 2024

jppipa, pendidikan ipa, fisika, biologi, kimia

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Medicinal plants are considered a rich source of bioactive metabolites and have the potential for the discovery and development of new drugs, one of which is the purple leaf plant which is known to treat various diseases. The aim of this research was to determine the effect of administering purple leaf ethanol extract over a certain period of time at doses of 500 mg/kgBB, 2.000 mg/kgBB and 5.000 mg/kgBB on the histopathology of the Wistar rat pancreas. In this study, an experimental method was used with a randomized block design, using 20 male Wistar rats which were divided into 4 research groups, 1 control group which was given Na-CMC. and 3 treatment groups were given purple leaf ethanol extract at a dose of 500 mg/kgBB (P1), dose of 2.000 mg/kgBB (P2) and dose of 5.000 mg/kgBB (P3). The test preparation was administered orally, for 28 days. The results of subchronic toxicity testing of purple leaf ethanol extract on Wistar rats carried out for 28 days, up to a dose of 5.000 mg/kg BB, showed that at a dose of 2.000 mg/kg BB – 5.000 mg/kg BB it was toxic to the histopathology of the Wistar rats pancreas, which was characterized by the presence of cell degeneration, increased organ index and the presence of symptoms of toxicity

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UHPLC-MS/MS phytochemical screening, polyphenolic content and antioxidant potential of Diplorhynchus condylocarpon (Müll.Arg.) Pichon (Apocynaceae), a medicinal plant

Cited by 6 publications

References 40 publications

Unlocking the hepatoprotective potential of the parasitic plant Orobanche foetida Poir. aqueous extract against CCl4-induced liver injury in rat

Unlocking the hepatoprotective potential of the parasitic plant Orobanche foetida Poir. aqueous extract against CCl4-induced liver injury in rat

Phytochemical and Biological Investigations of <em>Ficus carica</em> L. Leaf Extract Along with Formulation Development

Subchronic Toxicity Test of Purple Leaves Ethanol Extract (PLEE) on the Histopathological Picture of the Pancreas of Wistar Rats

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