UHPLC-QTOF-IMS-Based Metabolite Fingerprinting of Underutilized Cordia myxa Fruits and Leaves: A Nutraceutical Source

Abstract

The present investigation focused on the chemical composition and metabolomics of Cordia myxa fruits and leaves collected from five locations in Himachal Pradesh, India. Both fruits and leaves exhibited a substantial amount of phenolics in the range of 9.65−26.22 mg GAE/g and 5.35−33.28 mg GAE/g, respectively, followed by appreciable flavonoid content. Higher antioxidant activity was detected in Shahpur leaf extract (SLE) (IC 50ABTS 0.27 mg/mL) and Raja ka Talab fruit extract (RFE) (IC 50ABTS 0.21 mg/mL) among different harvesting locations of C. myxa. A higher amount of vanillic acid and rutin was detected in Nagrota Surian fruits extract (NFE) by ultraperformance liquid chromatography quadruple time-of-flight ion mobility separation (UHPLC-Q-TOF-IMS). However, glycine, alanine, and isoleucine (ile) were also quantified at higher levels. Fruit extracts exhibited a promising amount of ascorbic acid and macroelements and microelements, whereas a total of 44 unknown compounds were identified by ultraperformance liquid chromatography quadruple time-of-flight ion mobility separation (UHPLC-Q-TOF-IMS) based on a nontargeted approach. This comprehensive metabolomic report brings new insights into metabolite distribution and its possible future implementations in food and nutraceuticals.

The effects of the ethanol extract of Cordia myxa leaves on the cognitive function in mice

“…Accordingly, the antioxidant activity of ELCM was also higher than that of C. myxa fruit extract (ELCM, 336.58 ± 19.37 mg/ml; C. myxa fruit extract, IC 50 > 6,000 mg/ml) [ 34 ]. Recently, it has been reported that rutin is contained together with p -coumaric acid or caffeic acid in the C. myxa leaves extract [ 35 ]. We further identified the presence of rutin together with rosmarinic acid in ELCM (supplementary Fig.…”

Insights into the growth parameters, mineral composition, protein and UHPLC–QTOF–LC/MS‐based non‐targeted metabolomic fingerprints of wheatgrass as a function of photoperiod and temperature

“…The parameters of mass spectrometry were previously described by Kumar et al . (2022). Furthermore, the collective information of WJP extract in the form of .cef file were analysed with mass profiler professional (MPP) software (Agilent Technology, version B.12.00) for extensive data mining (Dadwal et al ., 2022).…”

“…The gradient elution conditions were -A (90%) and B (10%) from 0 to 7 min; A (10%) and B (90%) from 7 to 17 min; and A (90%) and B (10%) from 17 to 24 min at a flow rate of 0.2 mL min −1 . The parameters of mass spectrometry were previously described by Kumar et al (2022). Furthermore, the collective information of WJP extract in the form of .cef file were analysed with MASS PROFILER PROFESSIONAL (MPP) software (Agilent Technology, version B.12.00) for extensive data mining (Dadwal et al, 2022).…”

Wild Edible Flowers of Western Himalayas: Nutritional Characterization, UHPLC-QTOF-IMS-Based Phytochemical Profiling, Antioxidant Properties, and In Vitro Bioaccessibility of Polyphenols

“…, solution A comprising 0.1% formic acid in water and solution B comprising 0.1% formic acid in acetonitrile (ACN), was used at a flow rate of 0.30 mL/min for separation of metabolites as explained in Kumar et al and Dadwal et al. , The peaks were monitored at 280 nm, and the quantification of phenolic acids and flavonoids was performed by plotting the calibration curve for each standard at different concentrations along with their respective retention times and UV spectra as per ICH guidelines. − The linearity range, regression equation with r 2 values, LOD, and LOQ of individual phenolic acids and flavonoids are presented in Table S6.…”

“…The metabolites were separated and identified using high-resolution 6560 Ion Mobility Q-TOF LC/MS (Agilent, Santa Clara, USA). The technique uses a two-solvent system with mobile phases A (0.1% formic acid in water) and B (0.1% formic acid in acetonitrile) in a gradient elution condition, as mentioned in Kumar et al The parameters of mass spectrometry were explained earlier . Furthermore, the collective sample information was processed with MassHunter Qualitative Analysis software (version B.06.00, Agilent Technology) for extensive data mining .…”