UHPLC–QTOF–MS Metabolic Profiling of Marchantia polymorpha and Evaluation of Its Hepatoprotective Activity Using Paracetamol-Induced Liver Injury in Mice

Abstract

Marchantia species were traditionally used to treat liver failure. Marchantia polymorpha chloroform extract showed a marked hepatoprotective activity in a dose-dependent manner in paracetamol-induced extensive liver damage in mice. At a dose of 500 mg/kg (MP-500), it resulted in a reduction in aspartate transaminase by 49.44%, alanine transaminase by 44.11%, and alkaline phosphatase by 24.4% with significant elevation in total proteins by 58.69% with respect to the diseased group. It showed significant reductions in total bilirubin, total cholesterol, triglycerides, low density lipoprotein (LDL), very LDL, total lipids, and to high density lipoprotein ratio (CH/HDL) by 53.42, 30.14, 35.02, 45.79, 34.74, 41.45, and 49.52%, respectively, together with a 37.69% increase in HDL with respect to the diseased group. It also showed an elevation of superoxide dismutase by 28.09% and in glutathione peroxidase by 81.83% in addition to the reduction of lipid peroxidation by 17.95% as compared to the paracetamol only treated group. This was further supported by histopathological examination that showed normal liver architecture and a normal sinusoidal gap. Metabolic profiling by ultrahigh performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometer (UHPLC–QTOF/MS) led to the tentative identification of 28 compounds belonging to phenols, quinolones, phenylpropanoid, acylaminosugars, terpenoids, lipids, and fatty acids to which the activity was attributed. Four compounds were detected in the negative ionization mode which are neoacrimarine J, marchantin A, chitobiose, and phellodensin F, while the rest were detected in the positive mode. Thus, it can be concluded that this plant could serve as a valuable choice for the treatment of hepatotoxicity that further consolidated its traditional use.

Integrated transcriptome and metabolome revealed the drought responsive metabolic pathways in Oriental Lily (Lilium L.)

“…The identified metabolites were annotated using HMDB ( Wishart et al, 2022 ), massbank ( Horai et al, 2010 ), LipidMaps ( Sud et al, 2007 ), mzcloud ( Abdelrazig et al, 2020 ) and KEGG databases, ( Kanehisa et al, 2022 ). The parameter was set to ppm <30 ppm ( Asif et al, 2023 ).…”

Recent Advances in the Phytochemistry of Bryophytes: Distribution, Structures and Biological Activity of Bibenzyl and Bisbibenzyl Compounds

“…The main attributes, i.e., hepatoprotective activity, of these compounds are reported against hepatocellular injury caused by paracetamol-treated mice in dose-dependent manner, though preclinical studies are required to strengthen this conclusion [ 142 ].…”

“…The main attributes, i.e., hepatoprotective activity, of these compounds are found in hepatocellular injury caused by paracetamol-treated mice in dose-dependent manner, though preclinical studies are required to strengthen this conclusion [142]. Almost all Radula species belonging to the Radulaceae are chemically very isolated from the other genera of liverworts, since they produce bibenzyls, prenyl bibenzyls and/or bisbibenzyls, along with their related compounds as major components, many of which possess cytotoxic effects against various cancer cell lines [79].…”