Uji Aktivitas Antibakteri Campuran Ekstrak Biji Kelor (Moringa oleifera) dan Daun Kersen (Muntingia calabura) terhadap Pseudomonas aeruginosa dan Bacillus subtilis

Abstract

Moringa oleifera and Muntingia calabura leaves have been reported to have an antibacterial activity that could inhibit the growth of gram positive and negative bacteria. However, the antibacterial activity of mixed extracts of M. oleifera seeds and M. calabura leaves has not been widely reported. The purpose of this study was to test antibacterial activity of the mixed extract of M. oleifera seeds and M. calabura leaves on the growth of Pseudomonas aeruginosa and Bacillus subtilis. The experiment was conducted by agar disc diffusion method using three groups of extract treatments i.e. M.oleifera seeds extract, M.calabura leaves extract, and mixed extracts of M. oleifera seeds and M. calabura leaves with a ratio of 1: 1 (v / v). The extraction of M. oleifera seeds and M. calabura leaves was conducted by soxhlation method and using ethanol as solvent. The three groups of extract treatments with a concentration of 400 ppm, 800 ppm, 1200 ppm, and 1600 ppm were tested on P. aeruginosa. The antibacterial activity test of M. oleifera seed extract against B. subtilis carried out at the level of concentrations i.e. 150 ppm, 300 ppm, 450 ppm, 600 ppm, and 750 ppm, meanwhile, M. calabura leaves extract was done at concentration 1500 ppm, 3000 ppm, 4500 ppm, 6000 ppm, and 7500 ppm. The result showed that the three groups of extract treatments possess antibacterial activity against P. aeruginosa. The mixed extracts of M. oleifera seeds and M. calabura leaves with a ratio of 1: 1 (v / v) at level concentration of 400 ppm and 800 ppm were tested against P. aeruginosa significantly increased, and at concentrations of 1200 ppm and 1600 ppm significantly decreased the inhibition diameter of bacterial growth than the other extracts treatments. The antibacterial test results of M.oleifera seeds extract and M.calabura leaves extract against B. subtilis shows that increased concentrations of the extract significantly increase the inhibition diameter of bacterial growth especially at high concentrations ( 600 ppm and 750 ppm) on M. oleifera seeds extract, as well as 6000 ppm and 7500 ppm in M. calabura leaves extract.

Moringa oleifera Distribution in Java and Lesser Sunda Islands which is Attributed with Annual Rainfall

“…Moringa is reported to have many benefits, some of them are to be used as medicines (Wiguna, 2018), anti-oxidants (Badriyah et al, 2017), anti-bacterial (Suriaman & Khasanah, 2017;Wadji et al, 2017), anti-fungi (Kurniawan, 2015), bio-pesticides (Suwahyono, 2008), bio-stimulant (Culver et al, 2012;Abdalla, 2013), improving quality of water (Susanto et al, 2007;Suwahyono, 2008;Yuliastri, 2010) and waste water treatment (Januardi et al, 2014). Moringa also has been used as animal feed (Sjofjan, 2008;Suwahyono, 2008), improved nutrition of food (Zakaria et al, 2016), organic fertilizer, pulp materials, oil (Suwahyono, 2008), raw materials of soap (Argohartono & Wiguna, 2018) and as vegetables (Suwahyono, 2008).…”

Uji Daya Hambat Ekstrak Kasar Daun Johar (Cassia siamea L.) Terhadap Bakteri Pseudomonas aeruginosa

“…Ikan yang terinfeksi menunjukkan gejala klinis luka pada permukaan tubuh dan kulit. Infeksi penyakit ini juga menyebabkan lukai pada sirip dan ekor [3]. Karakteristik gejala klinis ditimbulkan oleh bakteri P. aeruginosai pada ikan yang terinfeksi adalah pendarahan bagian mulut, operkulum serta bagian sisi peruti tubuh [4].…”