UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAMANDRAH (Croton tilgium L.) Terhadap Pertumbuhan Salmonella typhi Dengan Metode Difusi Cakram (Kirby-Bauer)

Samputri, Revina Destiana; Toemon, Angeline Novia; Widayati, Ratna

doi:10.30595/hmj.v3i3.6393

Cited by 4 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Aktivitas penghambatan alkaloid yaitu dengan menganggu proses penyusunan peptidoglikan, sehingga mengakibatkan tidak terbentuknya lapisan pada dinding sel dan mengakibatkan kematian pada sel bakteri (Anggraini et al, 2019). Tanin dapat menginaktifkan enzim sel bakteri dan adhesin, dan mengganggu proses transport protein serta menganggu proses pembentukan polipeptida sehingga dinding sel yang terbentuk tidak sempurna dan akan terjadi lisis (Samputri et al, 2020) Kandungan senyawa fenolik dalam ekstrak kayu bidara laut memiliki mekanisme mendenaturasi protein pada sel bakteri. Struktur protein akan rusak karena terbentuknya ikatan hidrogen antara protein dan fenol.…”

Section: Uji Aktivitas Antibakteriunclassified

See 1 more Smart Citation

Antibacterial Activity of Ethanol, Ethyl Acetate, and n-Hexane Extracts of Bidara Laut Lignum (Strychnos lucida R.Br) against Staphylococcus aureus and Escherichia coli

Setyaningsih,

Bahri,

Nurjanah

2024

J Jamu Indo

View full text Add to dashboard Cite

Strychnos lucida is empirically believed to have efficacy in treating various diseases such as fever, malaria, toothache, and stomachache and to treat wounds. The secondary metabolite of Strychnos lucida has various polarity. The purpose of this study was to compare the inhibitory activity of Strychnos lucida extracts against E. coli and S. aureus bacteria. The test was carried out by measuring the Diameter of the Inhibitory Region (DIR) using the disc diffusion method on a concentration of 90%, 75%, 60%, 45%, 30%, and 15%. The Minimum Inhibitory Concentration (MIC) was carried out by agar dilution method on a concentration of 15%, 12.5%, 10%, 7.5%, 5%, and 2.5%. The positive control was 1% ampicillin, and the negative control was 10% DMSO. The ethyl acetate extract showed the highest DIR of 14.30 mm against S. aureus and E. coli on a concentration of 45%, ethanol extract was 8,38 mm against S. aureus and 8.71 mm against E. coli, and n-hexane had no inhibitory activity on a concentration 45%. The result of the MIC test for ethanol and ethyl acetate extract for both bacteria was found at a concentration of 15%, while n-hexane extract against Staphylococcus aureus was found at a concentration of 90% and a concentration of 75% against Escherichia coli.

show abstract

Section: Uji Aktivitas Antibakteriunclassified

“…Permeabilitas membran sitoplasma dan dinding sel akan terganggu dan terjadi ketidakseimbangan pada makromolekul dan ion sehingga mengakibatkan lisis (Widowati et al, 2019). Flavonoid menyebabkan rusaknya membran sel dan keluarnya senyawa intraseluler bakteri (Samputri et al, 2020).…”

Section: Uji Aktivitas Antibakteriunclassified

Antibacterial Activity of Ethanol, Ethyl Acetate, and n-Hexane Extracts of Bidara Laut Lignum (Strychnos lucida R.Br) against Staphylococcus aureus and Escherichia coli

Setyaningsih,

Bahri,

Nurjanah

2024

J Jamu Indo

View full text Add to dashboard Cite

show abstract

“…Penentuan (Samputri et al, 2020). Kontrol positif obat kloramfenikol (untuk S. typhi) dibuat menggunakan sediaan kapsul obat kloramfenikol ditimbang 0,05 g kemudian dilarutkan dengan 10 mL larutan DMSO 10%.…”

Section: Penentuan Minimum Inhibitory Concentration (Mic) Dan Minimum...unclassified

“…Kontrol positif obat kloramfenikol (untuk S. typhi) dibuat menggunakan sediaan kapsul obat kloramfenikol ditimbang 0,05 g kemudian dilarutkan dengan 10 mL larutan DMSO 10%. Kontrol negatif yang digunakan, yaitu DMSO karena tidak mempunyai pengaruh terhadap pertumbuhan mikroba uji (Samputri et al, 2020). Pelarut yang digunakan untuk melarutkan sampel pengujian yaitu larutan DMSO 10%.…”

Section: Penentuan Minimum Inhibitory Concentration (Mic) Dan Minimum...unclassified

Aktivitas Antimikroba Ekstrak Biji Rotan Manau (Calamus manan Miq.) Terhadap Salmonella typhi dan Candida albicans

Mahdiyah,

Maulina,

Hakim

et al. 2024

Al-Kauniyah J. Biol.

View full text Add to dashboard Cite

AbstrakDemam tifoid adalah penyakit demam akut yang disebabkan oleh infeksi bakteri Salmonella typhi. Kandidiasis oral merupakan infeksi yang disebabkan oleh fungi Candida albicans yang banyak terdapat pada mukosa rongga mulut. Tujuan dari penelitian yaitu mengidentifikasi aktivitas antimikroba ekstrak biji rotan manau (Calamus manan Miq.) terhadap S. typhi dan C. albicans. Metode ekstraksi yang digunakan adalah metode maserasi dengan pelarut etanol 96%. Skrining antimikroba ekstrak biji rotan manau menggunakan metode well-diffusion. Identifikasi aktivitas antimikroba ekstrak biji rotan manau terhadap S. typhi dan C. albicans untuk melihat Minimum Inhibitory Concentration (MIC) menggunakan metode broth dilution, sedangkan untuk melihat Minimum Bactericidal Concentration (MBC) terhadap S. typhi dan Minimum Fungisidal Concentration (MFC) terhadap C. albicans menggunakan metode solid dilution. Hasil skrining antimikroba didapatkan zona hambat pada ekstrak biji rotan manau terhadap S. typhi sebesar 21,39 mm dan terhadap C. albicans sebesar 16,14 mm. Nilai MIC ekstrak biji rotan manau pada konsentrasi 50% terhadap S. typhi dan C. albicans, sedangkan untuk MBC terhadap S. typhi maupun MFC terhadap C. albicans dari ekstrak biji rotan manau tidak ditemukan karena pada media padat masih ditemukannya pertumbuhan mikroba. Hasil penelitian ini diharapkan dapat menjadi acuan untuk penelitian lanjutan sebagai temuan awal senyawa potensi antimikroba dari ekstrak biji rotan manau terhadap bakteri dan fungi.AbstractTyphoid fever is an acute febrile illness caused by infection with the bacterium Salmonella typhi. Oral candidiasis is an infection caused by the fungus Candida albicans which is abundant in the oral mucosa. The purpose of this study was to identify the antimicrobial activity of rattan manau seed extract (Calamus manan Miq.) against the bacteria Salmonella typhi and the fungus C. albicans. The extraction method used is the maceration method. Antimicrobial screening of rattan manau seed extract using the well-diffusion method. Identification of antimicrobial activity of rattan manau seed extract against S. typhi bacteria and C. albicans fungi to see Minimum Inhibitory Concentration (MIC) using the broth dilution method, while to see Minimum Bactericidal Concentration (MBC) against S. typhi bacteria and Minimum Fungicidal Concentration (MFC) against the fungus C. albicans using the solid dilution method. The results of antimicrobial screening showed that the inhibition zone in the extract of rattan manau against S. typhi was 21.39 mm and against C. albicans was 16.14 mm. The MIC value of rattan manau seed extract was obtained at a concentration of 50% against S. typhi bacteria and C. albicans fungi, while for MBC against S. typhi and MFC against C. albicans from rattan manau seed extract not found because in solid media microbial growth is still found. The results of this study are expected to be a reference for further research as initial findings of antimicrobial potential compounds from the extract of manau rattan seeds against bacteria and fungi.

show abstract

“…Pada penelitian ini, diperoleh rata-rata daya hambat kloramfenicol 30 µg sebesar 15,47 mm, data tersebut tidak sesuai dengan penelitian Samputri et al, (2020) yang melaporkan bahwa rata -rata daya hambat kloramfenicol 30 µg terhadap Salmonella typhi sebesar 28,2 mm. Sedangkan penelitian Trisharyanti (2017), melaporkan bahwa bakteri Salmonella typhi mengalami resistensi terhadap kloramfenicol 30 µg dengan zona hambat sebesar 6,00 mm, sehingga penelitian ini sesuai dengan penelitian tersebut.…”

Section: Pembahasanunclassified

Antibacterial Activity of Mangosteen Bark Fraction (Garcinia Mangostana L.) Salmonella Typhi Atcc 13311

Anggraini¹,

Erikania²,

Maritha³

2022

J Vocat Heal Stud

View full text Add to dashboard Cite

Background: Typhoid fever is caused by Salmonella typhi bacterial infection which spreads via the faecal-oral route and has epidemic potential. Salmonella typhi is a Gram-negative bacterium in the Enterobacteriaceae family. Currently, the use of natural ingredients can be an alternative treatment for typhoid fever, one of which is the mangosteen stem skin (Garcinia mangostana L.). Purpose: To determine the antibacterial activity of mangosteen stem skin fraction against Salmonella typhi ATCC 13311. Method:The maceration method was selected using methanol solvent and fractionation by ECC using ethyl acetate and n-hexane as solvents. This study used five treatments (fraction with a concentration of 20%, 40%, 80%, chloramphenicol 30µg as a positive control, and DMSO as a negative control) which tested their antibacterial activity against Salmonella typhi ATCC 13311 by disc diffusion. Result: The results showed that the ethyl acetate fraction of mangosteen stem skin had the greatest average inhibition zone, that is 13.86 ± 0.72 mm compared to the n-hexane fraction of mangosteen stem skin with an inhibition zone of 10.43 ± 0.73 mm. The data obtained were then analyzed with One-way ANOVA. Conclusion: The results obtained were p=0.000 (sig <0.05), indicating that there was a significant difference between the ethyl acetate fraction and the n-hexane fraction with 20%, 60%, and 80% concentrations with positive control

show abstract

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KAMANDRAH (Croton tilgium L.) Terhadap Pertumbuhan Salmonella typhi Dengan Metode Difusi Cakram (Kirby-Bauer)

Cited by 4 publications

References 0 publications

Antibacterial Activity of Ethanol, Ethyl Acetate, and n-Hexane Extracts of Bidara Laut Lignum (Strychnos lucida R.Br) against Staphylococcus aureus and Escherichia coli

Antibacterial Activity of Ethanol, Ethyl Acetate, and n-Hexane Extracts of Bidara Laut Lignum (Strychnos lucida R.Br) against Staphylococcus aureus and Escherichia coli

Aktivitas Antimikroba Ekstrak Biji Rotan Manau (Calamus manan Miq.) Terhadap Salmonella typhi dan Candida albicans

Antibacterial Activity of Mangosteen Bark Fraction (Garcinia Mangostana L.) Salmonella Typhi Atcc 13311

Contact Info

Product

Resources

About