UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN SIRIH HIJAU (Piper betle L.) TERHADAP BAKTERI Streptococcus pyogenes dan Staphylococcus aureus

Sujono, Hernandi; Rizal, Samsu; Purbaya, Sari; Jasmansyah, Jasmansyah

doi:10.26874/jkk.v2i1.27

Cited by 6 publications

(4 citation statements)

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“…The obtained essential oil exhibited a yield of 0.21% v/w. The results that the essential oil content in green betel leaves exceeded that reported by Sujono et al [25], although the value was smaller compared to that study, which ranged from 0.25 -0.53% [26,27]. This variation can be attributed to numerous factors, including soil fertility, plant age, climate, storage conditions, drying techniques, chopping size, sample condition, and the method of distillation [28].…”

Section: Isolation and Determination Physical Properties Of Essential...mentioning

confidence: 72%

Isolation, Identification, and Antibacterial Testing of Essential Oil from Green Betel Leaf (Piper Betle L.) Using Well Diffusion Method

Fachriyah,

Fadillah,

Sarjono

et al. 2023

J. Kim. Sains Apl.

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Green betel leaves have been traditionally employed within communities for various medicinal purposes, owing to their rich composition. These leaves are endowed with secondary metabolites encompassing flavonoids, phenols, saponins, and essential oils. Notably, the essential oils within green betel leaves possess a spectrum of biological properties, including antioxidant, antifungal, antidiabetic, anti-inflammatory, antibacterial, antitumor, anti-Alzheimer's, and anti-carcinogenic activities. In this study, the essential oil from green betel leaf collected from Klaten, Central Java, Indonesia, was isolated using water-steam distillation. The components of this isolate were identified using GC-MS analysis. Antibacterial activity was assessed against Staphylococcus epidermidis ATCC 12228 (S. epidermidis) and Escherichia coli (E. coli) using the well-diffusion method at various concentrations (25%, 50%, 75%, 90%, and 100%). Chloramphenicol served as the positive control, while a solution of Tween 20 in distilled water used as the negative control. The essential oil derived from green betel leaves exhibited a brownish-yellow color, possessed a distinctive betel aroma, and had a concentration of 0.21% v/w, a refractive index of 1.5001, and a specific gravity of 0.961 g/mL. The GC-MS analysis revealed the presence of 44 components, with the five most abundant constituents being acetyl chavicol (20.65%), germacrene- D (11.55%), eugenol (8.94%), trans-caryophyllene (7.92%), and chavicol (5.74%). Regarding antibacterial activity, the isolate demonstrated strong activity against S. epidermidis ATCC 12228 at a concentration of 75%, yielding an inhibition zone diameter of 12.33 mm. Similarly, it exhibited strong activity against E. coli at a concentration of 90%, resulting in an inhibition zone diameter of 12.67 mm.

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Section: Isolation and Determination Physical Properties Of Essential...mentioning

confidence: 72%

Isolation, Identification, and Antibacterial Testing of Essential Oil from Green Betel Leaf (Piper Betle L.) Using Well Diffusion Method

Fachriyah,

Fadillah,

Sarjono

et al. 2023

J. Kim. Sains Apl.

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“…At higher concentrations, it is possible that more active compounds contained in plant preparations can be extracted and work as antibacterial [19]. Antibacterial compounds contained in betel and basil leaves played a role in inhibiting the growth of the test bacteria, and their effectiveness increased according to the increase in treatment concentration [20]. This study obtained a small number of bacterial colonies after giving each treatment concentration.…”

Section: Resultsmentioning

confidence: 94%

THE ANTIBACTERIAL ACTIVITY OF BETEL (Piper battle L.) AND BASIL (Ocimum sanctum L.) LEAVES INFUSION AS ANTISEPTIC PREPARATIONS AGAINST SOME BACTERIA IN VITRO

Fatimah

Budiarti

Medika

2022

BBRJ

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Betel leaves (Piper battle L.) and basil leaves (Ocimum sanctum L.) are parts of the plant that can be made as antiseptic preparations because they contain antibacterial compounds such as flavonoids, tannins, and saponins. Antiseptic activity can be observed based on its effect in inhibiting the colonization of standard test bacteria of the laboratory, namely Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi as well as the magnitude of the phenol coefficient value. The study aimed to analyze the infusion activity of betel and basil leaves as antiseptic preparations against some test bacteria. This study in vitro used a posttest-only control group design. The infusion treatment of betel and basil leaves is tested in the form of a single preparation and a combination with concentrations of 50%, 75%, and 100% (w/v). The results showed there was a difference in the infusion activity of betel leaves and basil leaves in a single stock and a combination of the number of bacterial colonies tested; the effect of the treatment produces a small number of bacterial colonies in Gram-positive bacteria; 100% combination treatment (b/v) provides an inhibitory effect that is not significantly different from 70% alcohol control against all test bacteria except S.typhi: and obtained the phenol coefficient value from the combination treatment of betel and basil leaves infusion higher against Gram-positive bacteria. The conclusion of this study, the combination of betel and basil leaves infusion has antibacterial activity as an antiseptic preparation that can inhibit the growth of some test bacteria. Keywords: antiseptic preparations, infusion leaves, Piper battle L, Ocimum sanctum L, number of colonies, phenol coefficient

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“…Dibuat dengan masing-masing konsentrasi 5%, 10%, 25%, dan 50% b/v dengan pelarur DMSO. Setelah seri konsentrasi dibuat paperdisk direndamkan didalam masing masing konsentrasi selama 15 menit [11].…”

Section: Penyiapan Sampel Ujiunclassified

Uji Aktivitas Antibakteri Minyak Atsiri Kulit Jeruk Pontianak (Citrus nobilis Lour. var. microcarpa) terhadap Bakteri Staphylococcus aureus dan Staphylococcus mutans

Ropiqa,

Ristia Rahman,

Kurniawan

et al. 2023

JSSCR

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Antibacterial is a substance that can inhibit the growth of bacteria and can kill bacteria that cause infection. Currently increasing resistance to antibiotics is an opportunity to utilize bioactive compounds from the diversity of plants in Indonesia, one of which is by utilizing Pontianak orange peels. Based on some literature, it is stated that essential oils from Pontianak orange peels can inhibit the growth of several types of bacteria. In this study, antibacterial tests were carried out on S.aureus and S.mutans bacteria. The essential oil of Pontianak orange peel was first tested for phytochemical screening and then an antibacterial test was carried out with several concentrations of 5%, 10%, 25%, and 50%, the antibacterial activity test was carried out using the disc diffusion method. The results of the phytochemical screening test showed that the essential oil of Pontianak orange peel contains flavonoids, saponins, and terpenoids. Furthermore, in testing the S.aerues bacteria, the essential oil of orange peel can have an inhibition zone with a range of 9-11 mm which is included in the strong antibacterial category, while in testing the inhibition of S. mutans bacteria, it is included in the moderate category, namely in the range of 7-10 mm. In the statistical test, there was no significant difference in the inhibition of S. mutans and S.aereus bacteria in inhibiting the growth of the orange peel essential oil as indicated by the diameter of the inhibition zone in the different treatment groups according to the concentration of orange peel essential oil.

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UJI AKTIVITAS ANTIBAKTERI MINYAK ATSIRI DAUN SIRIH HIJAU (Piper betle L.) TERHADAP BAKTERI Streptococcus pyogenes dan Staphylococcus aureus

Cited by 6 publications

References 0 publications

Isolation, Identification, and Antibacterial Testing of Essential Oil from Green Betel Leaf (Piper Betle L.) Using Well Diffusion Method

Isolation, Identification, and Antibacterial Testing of Essential Oil from Green Betel Leaf (Piper Betle L.) Using Well Diffusion Method

THE ANTIBACTERIAL ACTIVITY OF BETEL (Piper battle L.) AND BASIL (Ocimum sanctum L.) LEAVES INFUSION AS ANTISEPTIC PREPARATIONS AGAINST SOME BACTERIA IN VITRO

Uji Aktivitas Antibakteri Minyak Atsiri Kulit Jeruk Pontianak (Citrus nobilis Lour. var. microcarpa) terhadap Bakteri Staphylococcus aureus dan Staphylococcus mutans

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