Uji Aktivitas Antijamur Ekstrak Etanol Daun dan Daging  Buah Berenuk(Crescentia cujete, Linn.) terhadapCandida albicans ATCC 1023

Sulistyawati, Dewi; Wiryosoendjojo, Kartinah; Puspawati, Nony

doi:10.31001/biomedika.v12i2.616

Cited by 5 publications

(7 citation statements)

References 4 publications

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“…17 Alkaloid juga dapat menyebabkan lisis sel jamur, menghambat respirasi sel jamur, dan menghambat pembentukan asam nukleat yang dapat menghambat pertumbuhan jamur dan akan mengalami kematian sel. 28 Aktivitas tanin sebagai antimikroba ialah dengan menghambat pertumbuhan mikroba melalui khelasi zat besi sehingga sel-sel akan mengalami kekurangan zat besi, mengganggu metabolisme mikroba melalui penghambatan fosforilasi oksidatif, deprivasi senyawa penting untuk pertumbuhan, dan juga menghambat pembentukan enzim yang dibutuhkan dalam sitoplasma membran ekstrasel. (18) Saponin memiliki aktivitas antifungi terhadap Candida albicans dengan cara mengganggu sterol, menghambat transisi yeast ke hypal, dan menghambat pembentukan biofilm sel jamur.…”

Section: Bahasanunclassified

Efektivitas Ekstrak Daun Mangifera indica L. Menghambat Candida albicans pada Plat Resin Akrilik Heat-cured

Purbasari

Susanti

Lestarini

2023

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Heat-cured acrylic resin dentures are used to treat tooth loss, however, pores are easily formed on their surfaces which make it easier for Candida to adhere. Improper maintenance of dentures can cause overgrowth of Candida albicans and trigger denture stomatitis. This study aimed to determine the effectiveness of Mangifera indica L. extract on inhibition of Candida albicans on heat-cured acrylic resin plates. This was a true experimental laboratory study with a post-test only control group design. Samples were heat-cured acrylic resin plates contaminated with Candida albicans. The treatment groups were chlorhexidine gluconate 0.2%, aquades, and extract with concentrations of 65%, 75%, 85%, and 100%. Samples were immersed in the treatment groups for 15 minutes, followed by dilution and culture on SDA media, and then fungal colonies were observed. Data were analyzed with the Shapiro-Wilk normality test, the Levene's test for homogeneity, continued with the parametric one-way ANOVA and post hoc least significant difference tests. The results showed significant differences between the treatment groups (p<0.05). The percentage of the extract group with the lowest number of fungi was 75%, meanwhile the highest one was 65%. In conclusion, Mangifera indica L. leaf extract is effective in inhibiting the growth of Candida albicans on heat-cured acrylic resin plates with an optimal concentration of 75%. Keywords: mango leaf extract (Mangifera indica L.); Candida albicans; heat-cured acrylic resin Abstrak: Gigi tiruan resin akrilik heat-cured digunakan untuk mengatasi kehilangan gigi, namun sering terbentuk porus pada permukaannya yang mempermudah perlekatan Candida. Pemeliharaan gigi tiruan yang tidak tepat dapat menyebabkan pertumbuhan Candida albicans berlebihan dan memicu terjadinya denture stomatitis. Penelitian ini bertujuan untuk mengetahui efektivitas ekstrak daun mangga arum manis (Mangifera indica L.) dalam menghambat Candida albicans pada plat resin akrilik heat-cured. Jenis penelitian ialah true experimental laboratory dengan post test only control group design menggunakan sampel plat resin akrilik heat-cured yang dikontaminasi Candida albicans. Kelompok perlakuan yaitu chlorhexidine gluconate 0,2%, akuades, ekstrak konsentrasi 65%, 75%, 85%, dan 100%. Sampel direndam pada kelompok perlakuan selama 15 menit, dilanjutkan dengan tahap dilusi serta kultur pada media SDA, diakhiri dengan pengamatan koloni jamur. Analisis data menggunakan uji normalitas Shapiro Wilk, uji homogenitas Levene, dan dilanjutkan dengan uji parametrik One-Way ANOVA, dan Post Hoc Least Significant Difference. Hasil penelitian mendapat-kan perbedaan bermakna antar kelompok perlakuan (p<0,05). Kelompok ekstrak dengan angka jamur terendah ialah konsentrasi 75% dan yang tertinggi ialah konsentrasi 65%. Simpulan penelitian ini ialah ekstrak daun mangga arum manis (Mangifera indica L.) efektif dalam menghambat pertumbuhan Candida albicans pada plat resin akrilik heat-cured dengan konsentrasi optimal 75%. Kata kunci: ekstrak daun Mangifera indica L.; Candida albicans; resin akrilik heat-cured

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Section: Bahasanunclassified

Efektivitas Ekstrak Daun Mangifera indica L. Menghambat Candida albicans pada Plat Resin Akrilik Heat-cured

Purbasari

Susanti

Lestarini

2023

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“…Bandotan extract with a solvent temperature of 90℃ was able to inhibit the growth of the fungus Candida albicans. Compounds that can be bound are: flavonoids, alkaloids, and saponins, which can suppress fungal growth [16]. The results of the phytochemical analysis of bandotan extract conducted by researchers at room temperature are: Alkaloids and flavonoids.…”

Section: Anthracnose Intensity and Number Of Sick Fruitsmentioning

confidence: 99%

“…The results of previous studies that flavonoids can inhibit the growth of Candida albicans fungus by denaturing protein bonds on the cell membrane, the cell membrane will shrink when phenol enters the cell nucleus, causing the fungus to not grow. [16]. Flavonoids have a functional role in heat acclimatization of plants so that plants are drought resistant and tolerant to freezing, support flower color and aroma, attract pollinating insects which make fruit spread better.…”

Section: Phytochemical Testmentioning

confidence: 99%

The effectiveness of weed extract with different temperature aquades solvent for controlling antraknosa disease of red chili (Capsicum annum L.)

Budi,

Pribadi,

Fitri

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Red chili is an agricultural commodity with high economic value. National red chili production is still low and often does not meet the needs of the Indonesian people. One of the causes is anthracnose disorder. Red chili anthracnose caused by the fungus Colletotrichum capsici is characterized by symptoms of small black spots that can widen, dry out and shrink and the fruit can fall off. The aim of the study was to determine the effectiveness of extracts of several types of weeds with distilled water at different temperatures against anthracnose disease, growth, and yield of red chili plants. The study was a field experiment using a two-factor Completely Randomized Design (CRD). Factor 1 Types of weeds consist of: bandotan, kirinyuh, teki. Factor 2 Aquades Solvents with Different Temperatures consist of: room temperature, 50°C, 90°C. The variables observed were the intensity of anthracnose disease, plant height, number of healthy fruit, healthy fruit weight, number of sick fruit, phytotoxicity test and phytochemical screening test. The process of spraying botanical extracts is carried out once a week. The results showed that the type of weed extract treatment had a significant effect on the intensity of anthracnose disease and the number of sick fruits and had no significant effect on the number of healthy fruit, healthy fruit weight, and plant height. The treatment of distilled water with different temperatures did not significantly affect all observed variables. The interaction between the type of extract and the temperature of the solvent had a significant effect on the number of sick fruits. Bandotan extract treated with distilled water at room temperature produced the least number of sick fruits, namely 3.4 fruit/plant. The solvent temperature of 50°C produced more phytochemical compounds than the 3 types of weeds extracted. Red chili plants do not show any phytotoxic symptoms.

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“…Buah berenuk berwarna hijau dengan bentuk bulat-bundar dengan diameter mencapai 25 cm, daging buah berenuk mengandung bulir warna putih, bentuk biji yang kecil dan pipih berwarna putih (4). Daging buah berenuk biasanya digunakan secara tradisional oleh masyarakat untuk antelmitik (5), antijamur (6), analgesik (7), uretritis (8). Buah berenuk juga mengandung beberapa senyawa metabolit sekunder yaitu alkaloid, fenol, terpen (9), naptokuinon (10), saponin, flavonoid, tanin (6), antrakuinon dan cardenolid (11).…”

Section: Pendahuluanunclassified

“…Daging buah berenuk biasanya digunakan secara tradisional oleh masyarakat untuk antelmitik (5), antijamur (6), analgesik (7), uretritis (8). Buah berenuk juga mengandung beberapa senyawa metabolit sekunder yaitu alkaloid, fenol, terpen (9), naptokuinon (10), saponin, flavonoid, tanin (6), antrakuinon dan cardenolid (11). Kandungan nutrisi yang terdapat dalam buah berenuk yaitu karbohidrat dan gula sederhana seperti sukrosa, fruktosa dan galaktosa (12), protein, serat, vitamin A,B,C,E (13), kandungan mineral seperti kalsium, magnesium, zinc, potasium, sodium (11).…”

Section: Pendahuluanunclassified

Cytotoxic Activity Test Of Ethanolic Extract Of Berenuk Fruit (Crescentia Cujete L.) On Artemia Salina Leach Shrimp Larva Using Brine Shrimp Lethality Test (BSLT) Method

Suzana

Handayanti

2022

JFI

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Cancer is one of the leading causes of human death in the world. One of the therapies given to cancer patients is chemotherapy which has cytotoxic effects. One of the plants that have the same effect is berenuk or calabash (Crescentia cujete L.) plant, from the family of Bignoniaceae, which grows wild and is often referred to as a poisonous plant. This plant contains napthoquinone as one of cytotoxic chemical compounds and other metabolite secondary such as alkaloids, flavonoids, saponins, tannins, terpenoids, and anthraquinones. The objective of this study is to determine the cytotoxic activity of ethanolic extract of the fruit of berenuk on Artemia salina Leach larva. The BSLT (Brine Shrimp Lethality Test) method was used for this study. The powder of berenuk fruit flesh was extracted using ethanol. The obtained solvent was evaporated using a rotary evaporator to obtain a thick extract. The extract was tested on A. salina larva that has been prepared and the value of mortality was observed. The result of cytotoxic activity test of ethanolic extract of berenuk fruit on A. salina larva had the LC50 value of 529.386 ppm which is categorized as toxic.

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Uji Aktivitas Antijamur Ekstrak Etanol Daun dan Daging Buah Berenuk(Crescentia cujete, Linn.) terhadapCandida albicans ATCC 1023

Cited by 5 publications

References 4 publications

Efektivitas Ekstrak Daun Mangifera indica L. Menghambat Candida albicans pada Plat Resin Akrilik Heat-cured

Efektivitas Ekstrak Daun Mangifera indica L. Menghambat Candida albicans pada Plat Resin Akrilik Heat-cured

The effectiveness of weed extract with different temperature aquades solvent for controlling antraknosa disease of red chili (Capsicum annum L.)

Cytotoxic Activity Test Of Ethanolic Extract Of Berenuk Fruit (Crescentia Cujete L.) On Artemia Salina Leach Shrimp Larva Using Brine Shrimp Lethality Test (BSLT) Method

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