Uji Aktivitas Antijamur Sari Buah Belimbing Wuluh (Averrhoa bilimbi L.) Terhadap Jamur Candida albicans

Octaviani, Melzi; Fadila, Fadila

doi:10.22216/jk.v3i2.3309

Cited by 4 publications

(3 citation statements)

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“…bilimbi has also showed efficacy in addressing various health concerns, including asthma, diabetes, cough, fever, and throat soreness (Khoo et al, 2016). In line with previous studies, belimbing wuluh is a promising candidate for use as a functional food raw material due to its rich content of beneficial active compounds, including antioxidants, hypoallergenic, antibacterial, antidiabetic, anti-larvicidal, antifungal, and antiinflammatory components (Octaviani & Fadila, 2018). A comprehensive review study by Lakmal showed that the ethanol extract of its leaves could reduce blood glucose in streptozotocin (STZ)induced diabetic rats at a dose of 125 mg/kg BW.…”

Section: Introductionsupporting

confidence: 53%

Antidiabetic Activity of Averrhoa bilimbi L. Fruit Extracts and the Identification of Active Compounds Using LC-MS and In silico Methods

Widiastuti,

Sinaga,

Warnasih

et al. 2024

Indonesian J Pharm

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Patients with diabetes mellitus require drugs that do not cause side effects in long-term use. In this era, many people use herbal medicine to treat diabetes mellitus. One species we can use as herbal medicine is Averrhoa bilimbi L., a plant whose fruit is known as belimbing wuluh and is used because it has value as an alternative medicine. This study aims to determine the antidiabetic activity of the active fraction of Averrhoa bilimbi L. fruit in vitro and in silico and to obtain the active compounds contained in the fruit fraction of starfruit (A. bilimbi L.) using Liquid Chromatography Mass Spectrometer (LC-MS). Sixteen organic compounds were identified using LC-MS analysis of the n-hexane fraction, which had the highest IC50 (7.03 μg/mL) antidiabetic activity compared to ethyl acetate (102.77 μg/mL) and n-butanol (181.65 μg/mL). In silico molecular docking, analysis was performed for all identified compounds to support the antidiabetic characteristics further. Computational predictions in this investigation revealed that two A. bilimbi fruit compounds with the greatest RT value, namely 2,3,4,5,6-pentaphenylbenzazocine (-16.0 kcal/mol) and Thraustochytroside A (-6.2 kcal/mol), exhibited higher binding affinity values than miglitol (-5.4 kcal /mol). This indicates that both compounds have promising futures as α-glucosidase inhibitor alternatives for treating diabetic disorders.

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Section: Introductionsupporting

confidence: 53%

Antidiabetic Activity of Averrhoa bilimbi L. Fruit Extracts and the Identification of Active Compounds Using LC-MS and In silico Methods

Widiastuti,

Sinaga,

Warnasih

et al. 2024

Indonesian J Pharm

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“…1 mL ekstrak etanol pegagan dilarutkan ke dalam etanol 70% sebanyak 3 ml Ekstrak tersebut kemudian dikocok sembari dipanaskan, lalu disaring. Filtrat yang dihasilkan ditambahkan dengan 0,1 g bubuk magnesium dan beberapa tetes HCl pekat (Octaviani & Fadila, 2018). Kandungan flavonoid ditunjukkan dengan munculnya warna jingga, kuning, dan merah (Aziz, 2015).…”

Section: Uji Fitokimia Flavonoidunclassified

APLIKASI METODE BIOAUTOGRAFI DALAM PENELUSURAN DAYA ANTIBAKTERI EKSTRAK PEGAGAN (Centella asiatica (L.))

Sitepu

Nurdiani

Rollando

2020

J. Kat.

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Abstract Pegagan (Centella asiatica (L.) Urban) is a traditional herbal plant that spread as it grows and in bloom througout the year. Pegagan is believed to be able to cure various type of diseases because it contains bioactive component that’s good for human body. Pegagan’s bioactive component that has anti-bacterial properties are saponin, flavonoid, and tanin. This study was conducted with the purpose to determine the existence of antibacterial activity in ethanol extract of pegagan leaves by applying it in Disc Diffusion Test method against Escherichia coli and Staphylococcus aureus bacteria. Etanol extract was obtained by using maserasi method with 70% ethanol, separation of the active compound group which one of them is flavonoid using phytochemical screening, and the result was that the pegagan positively contains flavonoid, it was proven by the existence of a red marker on the tube. The result of active compund using methanol eluent: chloroform: glacial acetic acid produces 1 spot point on (Rf 0,5-0,7) on TLC. This spot was used in antibacterial screening by bioatugraphy method and the activity was detected qualitatively. Antibacterial activity was proved by Disc Diffusion Test which the results were ethanol extract on pegagan has inhibitory activity to E.coli and S. aureus. This study can be concluded that ethanol extract of Centella asiatica has a MIC value of 3,200 µg / mL both in E. Coli and S. aureus. The diameter of obstacles zone were 0,06±0,05 mm to E.coli and 0,04±0,019 mm to S. aureus. Abstrak Centella asiatica (L.) Urban (Pegagan) adalah spesies tumbuhan herbal tradisional dengan karakteristik tumbuh merambat dan berbunga di sepanjang tahun. Komponen bioaktif yang dimiliki pegagan dalam beberapa aspek, dapat digunakan dalam pengobatan penyakit. Golongan-golongan senyawa bioaktif pegagan yang memiliki daya antibakteri adalah saponin, flavonoid, dan tanin. Penelitian ini bertujuan untuk mengetahui adanya aktivitas antibakteri ekstrak etanol daun pegagan dengan metode Disc Diffusion Test pada Escherichia coli maupun Staphylococcus aureus. Ekstrak tanaman Pegagan diperoleh dengan maserasi dengan etanol 70%, pemisahan golongan senyawa aktif salah satunya flavonoid menggunakan skrining fitokimia, yang secara kualitatif menunjukkan ekstrak tanaman mengandung flavonoid. Hasil Kromatografi Lapis Tipis (KLT) senyawa aktif menggunakan eluen metanol: kloroform: asam asetat glasial menghasilkan 1 titik spot pada Rf 0,5-0,7 digunakan dalam penelusuran pengujian daya antibakteri dengan metode autobiografi dan menunjukkan adanya pembentukan zona bening. Pada uji kuantitatif, ekstrak etanol pegagan memiliki nilai KHM 3.200 µg/mL baik pada Eschericia coli maupun Staphylococcus aureus. Nilai Kadar Bunuh Minimum (KBM) lebih dari 6.400 µg/mL aktivitas daya hambat dengan diameter 0,06±0,05 mm pada E.coli dan 0,04±0,019 mm untuk S. aureus.

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“…Hasil pengujian skrining fitokimia yang telah dilakukan, didapatkan bahwa ekstrak etanol kulit nanas memiliki kandungan senyawa berupa fenolik, flavonoid, steroid, saponin dan alkaloid. Senyawa metabolit sekunder tersebut memiliki aktivitas sebagai antijamur (6,15,16). Senyawa alkaloid bekerja sebagai penghambat biosintesa asam nukleat pada jamur (17).…”

Section: Uji Aktivitas Antijamurunclassified

Aktivitas Antijamur Ekstrak Etanol Kulit Buah Ananas comosus (L) Merr. terhadap Trichophyton mentagrophytes dan Malassezia furfur

Octaviani¹,

Fikrani²,

Susanti³

2020

JFI

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Kulit buah nanas merupakan bagian dari buah nanas (Ananas comosus (L) Merr.) yang biasanya tidak dimanfaatkan. Hasil skrining fitokimia dari ekstrak etanol kulit nanas menunjukkan adanya kandungan flavonoid, alkaloid, fenolik, steroid dan saponin yang dapat menghambat pertumbuhan mikroorganisme. Tujuan penelitian ini adalah untuk mengetahui aktivitas antijamur ekstrak etanol kulit nanas terhadap jamur Trichophyton mentagrophytes dan Malassezia furfur. Penelitian dilakukan dengan metode difusi cakram dengan variasi konsentrasi ekstrak etanol kulit nanas yaitu 25%; 12,5%; 6,25%; 3,125% dan 1,5625% b/v dan kontrol positif yang digunakan adalah nistatin dan kontrol negatif DMSO. Diameter zona hambat yang terbentuk pada uji aktivitas antijamur ekstrak etanol kulit nanas terhadap jamur Trichophyton mentagrophytes dan Malassezia furfur pada konsentrasi 25% adalah 22,73 mm dan 24,90 mm. Hal ini menunjukkan bahwa ekstrak etanol kulit nanas memiliki aktivitas antijamur terhadap jamur Trichophyton mentagrophytes dan Malassezia furfur.

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Uji Aktivitas Antijamur Sari Buah Belimbing Wuluh (Averrhoa bilimbi L.) Terhadap Jamur Candida albicans

Cited by 4 publications

References 0 publications

Antidiabetic Activity of Averrhoa bilimbi L. Fruit Extracts and the Identification of Active Compounds Using LC-MS and In silico Methods

Antidiabetic Activity of Averrhoa bilimbi L. Fruit Extracts and the Identification of Active Compounds Using LC-MS and In silico Methods

APLIKASI METODE BIOAUTOGRAFI DALAM PENELUSURAN DAYA ANTIBAKTERI EKSTRAK PEGAGAN (Centella asiatica (L.))

Aktivitas Antijamur Ekstrak Etanol Kulit Buah Ananas comosus (L) Merr. terhadap Trichophyton mentagrophytes dan Malassezia furfur

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