Uji Aktivitas Ekstrak Daun Opo-Opo (Desmodium pulchellum Linn Benth) sebagai Antibakteri terhadap Pertumbuhan Streptococcus viridans dan Streptococcus pyogenes

Abstract

Streptococcal tonsillopharyngitis is a type of tonsillopharyngitis caused by group A streptococcal bacterial infection with common symptoms such as sore throat, fever more than 38oC, tonsilar exudate, and cervical adenopathy. Possible complications of streptococcal tonsillopharyngitis include rheumatic fever that occurs at weeks 1-5 after acute respiratory infections by Streptococcus viridans and Streptococcus pyogenes. Treatment needs to be done This research aims to determine the value of Minimum Inhibitory Concentration (MIC) and Minimum Kill Concentration (MKC) of Opo-opo leaf extract (Desmodium pulchellum Linn Benth) on the growth of Streptococcus viridans and Streptococcus pyogenes. This research uses a maceration extraction method of the ingredients. microbiological test and test using the liquid dilution method. The extract concentration used was 0.1% w / v, 0.25% w / v, 0.5% w / v, 0.75% w / v, 1% w / v, 1.25% w / v , 1.5% w / v, 1.75% w / v, 2% w / v, 2.25% w / v, 2.5% w / v with negative control of Na, CMC 1% w / v. The test results showed that the MIC of Opo-opo leaf extract on Streptococcus viridans was 0.75%, MKC of opo-opo leaf extract on Streptococcus viridaans was 1% while the MIC of Opo-opo leaf extract on Streptococcus pyogenes was 0.25% and the MKC of leaf extract. Opo-opo in Streptococcus pyogenes is 1%. The results of the phytochemical screening of Opo-opo leaf extract (Desmodium pulchellum Linn Benth) were positive for alkaloids, flavonoids, tannins and terpenoids.

PENGARUH PERBEDAAN METODE EKSTRAKSI TERHADAP UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL BIJI KETUMBAR (Coriandrum sativum) PADA Staphylococcus aureus

“…Sebanyak 1,3 gram media Nutrient broth dimasukkan ke dalam erlenmeyer kemudian ditambahkan dengan 100 mL aquades lalu dipanaskan hingga media terlarut. Kemudian dilakukan proses sterilisasi menggunakan autoklaf pada suhu 121˚C dengan tekanan 1 atm selama 15 menit) [13].…”

Antibacterial Potential of Ethanol Extract of Coriander Seeds (Coriander sativum) Against Staphylococcus aureus

“…NB media is made by mixing1.3 grams of media was then dissolved in 100 mL of distilled water and then heated using a hotplate until the media was dissolved. then sterilize the media using an autoclave at a temperature of 121˚C for 15 minutes (Isnaeni et al, 2021).…”