UJI ANTIOKSIDAN DAN UJI BSLT PADA EKSTRAK KASAR Holothuria scabra DENGAN BERBAGAI PELARUT

Abstract

Holothuria scabra is one of high-value sea cucumber. The beneficial aspect of sea cucumbers is determined by the source of raw materials. The extraction process is a process that happens before sea cucumbers are used. In this process, the use of a various solvent is not only to facilitate the production process but also to maintain the active material. Also, the amount of yield obtained in the extraction process becomes a consideration in the production process. One of the benefits that are often claimed is antioxidants. This study aims to determine the yield of sea cucumbers after the extraction process by maceration with various types of solvents then antioxidant testing was carried out with DPPH followed by BSLT test. It was commonly used and easy to do. Besides, BSLT test was carried out to determine the initial toxicity and potency to be developed as antibacterial, antifungal and anticancer candidates. Results showed that the highest yield was 8.98% produced by dry sea cucumber samples extracted with ethanol PA 70% ethanol (code T5). In the results of the antioxidant tests, the best IC50 comes from dried sea cucumber extracted using ethyl acetate (code T2 and T3) with 1140 ± 0.00 ppm and1140 ± 10 ppm respectively. In the BSLT test results, the lowest LC50 was shown by extract using 100% methanol PA solvent (T4 code), with 108 ppm.