UJI DAYA HAMBAT EKSTRAK DAUN SALAM (Syzygium polyanthum [Wight] Walp) TERHADAP PERTUMBUHAN BAKTERI Escherichia Coli

Utami, Putra Rahmadea

doi:10.36911/pannmed.v15i2.726

Cited by 7 publications

(10 citation statements)

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“…In the MBC test, the MBC value of bay leaf extract was found at a concentration of 500mg/ml which was indicated by the absence of growth of Escherichia coli bacteria on NA medium (Figure 1). According to research by Utami (2020) it is known that 96% ethanol extract of bay leaves can inhibit the growth of…”

Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of Bay Leaf (Syzygium polyanthum) Ethanol Extract on Escherichia coli Growth

Mursyida

Almira²,

Widiasari³

et al. 2021

Photon

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Acute diarrhea is liquid stool with a frequency of more than 3 times a day and lasts less than 14 days. One of the most common causes of acute diarrhea is Escherichia coli. Diarrhea caused by bacteria can be treated with the use of antibiotics, but the relatively high intensity of antibiotic use causes various health problems, especially bacterial resistance. Therefore, the search for antibacterial agents derived from natural ingredients was carried out as an alternative treatment. Bay leaf (Syzygium polyanthum) is one of the natural ingredients that has the potential as an antibacterial because it contains active compounds such as essential oils, flavonoids (quercetin), saponins, tannins, and alkaloids. This study aimed to analyze the antibacterial activity of the ethanolic extract of bay leaves on the growth of Escherichia coli. This research uses the dilution method which aims to determine the Minimum inhibitory concentration (MIC) and Minimum bactericidal concentration (MBC). The results showed that the MIC value at a concentration of 200mg/ml and the MBC value at a concentration of 500mg/ml.

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Section: Resultsmentioning

confidence: 99%

Antibacterial Activity of Bay Leaf (Syzygium polyanthum) Ethanol Extract on Escherichia coli Growth

Mursyida

Almira²,

Widiasari³

et al. 2021

Photon

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“…Discs containing antibacterial substances are placed on the surface of agar plates containing the organism being tested. Antibacterial effectiveness is indicated by the inhibition zone, whose diameter is measured with a calliper with units of millimetres (mm) [26].…”

Section: Disc Diffusionmentioning

confidence: 99%

Antibacterial Activity and Inhibition Mechanism of Red Ginger (Zingiber officinale var. rubrum) Ethanol Extract Against Pathogenic Bacteria

Juariah

Bakar²,

Bakar³

et al. 2023

ARASET

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Pathogenic bacteria can interfere with the body health by producing toxic substances, damaging body tissues, multiplying and killing healthy body cells. Therefore, it is crucial to explore safe alternative treatment strategies, such as developing natural bactericidal agents to eradicate infections. Red ginger (Zingiber officinale var. rubrum) has potential as an antibacterial, so it is important to study its activity in inhibiting pathogenic bacteria. The aim of this study was to assess the antibacterial activity and inhibition mechanism of red ginger extract as an inhibitor of pathogenic bacterial infection. Methods such as dilution, Kirby-Bauer disc diffusion, and an inhibition test mechanism using a spectrophotometer and SEM were conducted in this study. Red ginger ethanol extract can inhibit the growth of Salmonela thypi, Staphylococcus epidermidis, and Streptococcus mutans at a concentration of 500 µg/mL, while Pseudomonas aeruginosa at a concentration of 250 µg/mL. Further observation of bacterial cell leakage showed that the higher the red ginger ethanol extract concentration, the higher the bacterial cell leakage. Based on SEM results, the quantity of S. thypi, S. epidermidis, and S. mutans after treatment with red ginger ethanol extract decreased, and the cell wall became wrinkled and destroyed. The ethanol extract of the red ginger rhizome can be recommended as a future antibacterial agent, especially for infections caused by pathogenic bacteria.

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“…Pertumbuhan E. coli dapat terhambat disebabkan interaksi negatif antara zat antibakteri di dalam ekstrak daun coklat dengan komponen penyusun sel E. coli. Ekstrak daun T. cacao L. mengandung alkaloid, flavonoid, terpenoid dan saponin (Parbuntari et al, 2018) (Redwik et al, 2019;Utami, 2020). Menurut Mandhaki et al, (2021 diketahui bahwa daya hambat ekstrak etanol daun T. cacao L. pada Staphylococcus aureus lebih baik dari pada hambatannya terhadap E. coli.…”

Section: Pembahasan Daya Hambat Daun T Cacao L Terhadap Pertumbuhan E...unclassified

Uji Antibakteri Ekstrak Etanol Daun Theobroma cacao L. terhadap Pertumbuhan Escherichia coli dengan Metode Difusi Cakram

2022

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This study aims to determine the inhibition of T. cacao L. leaf extract on the growth of E. coli bacteria and the correlation between the concentration of T. cacao L. leaf extract and the formed E. coli inhibition zone. The method used is experimental post-test only control design using Completely Randomized Design (CRD). The inhibition zone of T. cacao L. leaves against E. coli was tested using the disc diffusion method with six treatments (concentration 25%, 50%, 75%, 100%, positive control and negative control) repeated five times. The results showed that the diameter of the inhibition zone of the leaf extract of T. cacao L. against E.coli was at intervals of 5-10 mm and was included in the category of moderate inhibition, but the results of Duncan's test showed that there was no significant difference between the inhibition zones formed at concentrations 25%, 50%, 75%, and 100%. In conclusion, the leaves of T. cacao L. were less effective in inhibiting the growth of E. Coli, but the leaf extract of T. cacao L. was positively correlated with the inhibition zone formed. Keywords: Leaf Extract, Escherichia coli, Theobroma cacao L., Zone of Inhibition Test

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UJI DAYA HAMBAT EKSTRAK DAUN SALAM (Syzygium polyanthum [Wight] Walp) TERHADAP PERTUMBUHAN BAKTERI Escherichia Coli

Cited by 7 publications

References 0 publications

Antibacterial Activity of Bay Leaf (Syzygium polyanthum) Ethanol Extract on Escherichia coli Growth

Antibacterial Activity of Bay Leaf (Syzygium polyanthum) Ethanol Extract on Escherichia coli Growth

Antibacterial Activity and Inhibition Mechanism of Red Ginger (Zingiber officinale var. rubrum) Ethanol Extract Against Pathogenic Bacteria

Uji Antibakteri Ekstrak Etanol Daun Theobroma cacao L. terhadap Pertumbuhan Escherichia coli dengan Metode Difusi Cakram

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