UJI KARAKTERISTIK FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN  BIJI KOPI ROBUSTA (Coffea canephora Pierre) DARI BOGOR, BANDUNG DAN GARUT DENGAN METODE DPPH (1,1-diphenyl-2-picrylhydrazyl)

Wigati, Evi Indah; Pratiwi, Esti; Nissa, Trisni Fatwatun; Utami, Novi Fajar

doi:10.33751/jf.v8i1.1172

Cited by 12 publications

(19 citation statements)

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“…Hasil skrining fitokimia dari ekstrak etanol kopi hijau robusta lampung disajikan pada Tabel 1, dan menunjukkan hasil positif pada uji flavonoid dan uji tanin. Hal ini sesuai dengan penelitian Wigati et al (2018) yang menyatakan bahwa komponen kimia pada kopi robusta mengandung golongan senyawa flavonoid, alkaloid, tanin dan saponin. Penelitian Chairgulprasert dan Kittiya (2017) juga menunjukkan bahwa komponen kimia pada kopi robusta mengandung alkaloid, tanin, saponin, flavonoid dan terpenoid.…”

Section: Skrining Fitokimiaunclassified

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Antiproliferation Activities of Ethanol Extract of Robusta Lampung Green Coffee Seeds on Dog Tumor Line Cells

et al. 2021

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This study aim at determining the activity of ethanol extract of Robusta green coffee beans as an antiproliferation agent in MCA-B1, MCM-B2, and Vero normal cell lines as a control. Ethanol extract of Robusta green coffee beans was obtained by maceration method, bioactive compounds in the extract were tested by phytochemical screening methods, testing levels of antioxidants were tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, cytotoxicity effects (LC50) were tested by the method Brine Shrimp Lethallity Test (BSLT) and antiproliferation activity were carried out by counting the number of cells after being tested with several extract concentrations namely 100, 250, 400, 550, 700 and 850 ppm and doxorubicin as a positive control with a concentration of 100 ppm. The results showed that the ethanol extract of Robusta green coffee beans contained flavonoids and tannins, had very strong antioxidant levels with IC50 values of 40.9923 ppm, cytotoxicity effects obtained LC50 values of 430.64 ppm, and the highest antiproliferation activity was achieved at a concentration of 850 ppm at 69.58 % in MCM-B2 tumor cells, 60.46% in MCA-B1 tumor cells, and 14.2% in Vero normal cells. Based on the results, the ethanol extract of Robusta Lampung green coffee beans has antiproliferation activity on the test tumor cells and is relatively non-toxic to normal cells.

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Section: Skrining Fitokimiaunclassified

“…Perbedaan hasil uji skrining fitokimia dengan penelitianpenelitian sebelumnya dipengaruhi oleh letak geografis sebaran tanaman. Letak geografis berpengaruh terhadap kondisi lingkungan seperti suhu udara, sinar matahari, kelembapan udara (Artanti et al, 2016), curah hujan dan ketinggian tempat tanam (Wigati et al, 2018).…”

Section: Skrining Fitokimiaunclassified

Antiproliferation Activities of Ethanol Extract of Robusta Lampung Green Coffee Seeds on Dog Tumor Line Cells

et al. 2021

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“…Bila pH sediaan terlalu asam dapat menyebabkan iritasi kulit dan bila pH terlalu basa dapat menyebabkan kulit menjadi kering bersisik sehingga mengurangi nilai estetika pada kulit. Nilai pH sesuai dengan standar berkisar antara 4,5-6,5 yang menyesuaikan pH pada kulit 23 . Setelah dilakukan pengukuran didapatkan nilai pH masing-masing sediaan masker gel peel-off tanpa dan dengan ekstrak sebesar 5,5 dan 5,3.…”

Section: Pembahasanunclassified

Formulasi Masker Gel Peel-Off Berbahan Ekstrak Biji Kopi Robusta (Coffea canephora) Khas Lampung

et al. 2022

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Kopi robusta (Coffea canephora) merupakan salah satu komoditas unggulan yang berada di Provinsi Lampung. Selain sebagai olahan minuman, biji kopi robusta juga dapat dimanfaatkan sebagai bahan tambahan alami dalam sediaan produk kosmetik. Masker gel peel-off merupakan salah satu jenis produk kosmetik yang dapat berfungsi sebagai anti-aging pada wajah. Pada penelitian ini, formulasi masker gel peel-off berhasil dipreparasi dengan memanfaatkan ekstrak biji kopi robusta. Metode formulasi masker gel peel-off dilakukan melalui proses in-situ. Hasil uji fitokimia menunjukkan senyawa metabolit sekunder yang terkandung dalam ekstrak biji kopi robusta yaitu alkaloid, flavonoid, saponin, dan tannin. Analisis spektrofotometer UV-vis menunjukkan kadar senyawa flavonoid sebesar 5,86 mg/g. Hasil uji pH masker gel peel-off menggunakan ekstrak kopi robusta sebesar 5,3. Masker gel peel-off tidak menyebabkan reaksi iritasi pada kulit. Sifat fisik dari masker gel peel-off terlihat berwarna coklat dengan bentuk semisolid. Hasil uji aktivitas antioksidan masker gel peel-off menunjukkan nilai IC50 sebesar 7,104 ppm. Dari data yang ada dapat disimpulkan bahwa senyawa flavonoid dalam ekstrak biji kopi robusta sangat berpengaruh terhadap aktivitas antioksidan dalam formulasi sediaan masker gel peel-off.

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“…The color of the DPPH radical from purple to yellow as measured by a spectrophotometer at a wavelength of about 515 nm (Prior et al, 2005), in this study the wavelength used is 517 nm. The 517 nm wavelength used is the same as the research by (Wigati et al, 2018). Antioxidant activity of ground coffee extract from respondents Rope coffee powder (R), PM Coffee (Tio), and Raosan Coffee (G).…”

Section: Antioxidant Activitymentioning

confidence: 99%

“…The IC50 value range is different for each processing method. The difference in antioxidant activity of each ground coffee extract from the difference in roasting temperature can be directly or in directly influenced by the active substances of secondary metabolites produced by plants (Wigati et al, 2018). The production of secondary metabolites in plants is influenced by several things including the climate and altitude of the planting site.…”

Section: Antioxidant Activitymentioning

confidence: 99%

Coffee Powder Antioxidant and Acrylamide Activity in Different Processing and Roasting Methods

Analianasari¹,

Berliana²,

Shintawati³

2022

proceeding

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This research aimed to analyze the antioxidant activity of processed ground coffee and the levels of acrylamide in roasted ground coffee at different temperature levels. The research method is a descriptive qualitative method by calculating antioxidant activity with UV-Vis spectrophotometer and acrylamide content by HPLC. The results showed that the levels of acrylamide produced from processing with different roasting levels were still below the tolerance limit and had strong antioxidant activity at various roasting levels with different processing methods. Keywords: Antioxidant, acrylamide, coffee powder

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UJI KARAKTERISTIK FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN BIJI KOPI ROBUSTA (Coffea canephora Pierre) DARI BOGOR, BANDUNG DAN GARUT DENGAN METODE DPPH (1,1-diphenyl-2-picrylhydrazyl)

Cited by 12 publications

References 0 publications

Antiproliferation Activities of Ethanol Extract of Robusta Lampung Green Coffee Seeds on Dog Tumor Line Cells

Antiproliferation Activities of Ethanol Extract of Robusta Lampung Green Coffee Seeds on Dog Tumor Line Cells

Formulasi Masker Gel Peel-Off Berbahan Ekstrak Biji Kopi Robusta (Coffea canephora) Khas Lampung

Coffee Powder Antioxidant and Acrylamide Activity in Different Processing and Roasting Methods

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