Abstract. Bahuwa IC, Lamondo D, Katili AS. 2022. Short Communication: Papaya (Carica papaya) seed extract test against Spodoptera litura mortality. Cell Biol Dev 6: 1-5. This study aims to determine the effect of papaya (Carica papaya L.) seed extract on mortality, the most influential concentration of papaya seed extract, Lethal Concentration (LC50) 24 hours, and Lethal Time (LT50) 24 hours armyworm (Spodoptera litura Fabricius, 1775) larvae instar III. The research was carried out at the Biology Department, Universitas Negeri Gorontalo, Indonesia, in June 2021. The method used was an experiment with a Completely Randomized Design consisting of 9 treatments and 3 replications, there are A (aquadest control), B (CMC control), C (1 ppm), D (50 ppm), E (100 ppm), F (200 ppm), G (400 ppm), H (800 ppm), and I (1600 ppm). The data were analyzed by probit analysis LC50, LT50, and One Way Anova. The results showed that the LC50 value was at a dose of 489 ppm. The value of LT50 24 hours at a concentration of 1600 ppm is 10.61 hours, with the fastest time to kill 50% of armyworm larvae. Papaya seed extract affects the mortality of armyworm larvae; the higher the concentration, the less time it takes to kill 50% of the larvae.