Uji Toksisitas Jamur Metarhizium anisopliae terhadap Hama Ulat Krop Kubis Crocidolomia binotalis Zell.

Abstract

Cabbage head caterpillar Crocidolomia binotalis Zell. caused up to 79.81% damage to cabbage plants. Metarhizium anisopliae is an entomopathogenic fungus that is often used for pest control in the field because it is toxic to various phases of insect life. This study aimed to determine the toxicity ability of the fungus Metarhizium anisopliae in controlling the population of the cabbage head caterpillar Crocidolomia binotalis Zell. in the laboratory. The research was conducted at the Laboratory of Agrotechnology, Faculty of Agriculture, University of Jember. The study used a Completely Randomized Design with 5 treatments and 4 replications in order to obtain 20 experimental units. The treatments used in this study were as follows: A = Control (aquadest); B = density 104 conidia/ml; C = density 105 conidia/ml; D = 106 conidia/ml; and E = 107 conidia/ml. The results showed that in the laboratory test the density of the fungus Metarhizium anisopliae 107 conidia/ml caused the highest mortality of 92.50%, while the lowest mortality percentage was 22.50% in the treatment of 104 conidia/ml on 7 days of observation. The results of the LT50 calculation showed that the most effective density was 106 conidia/ml with an LT50 value on the 4th day. The most effective density of Metarhizium anisopliae, 106 conidia/ml, was recommended for use in population control of the cabbage head caterpillar Crocidolomia binotalis Zell. because it is capable of causing the death of 50% of the population of Crocidolomia binotalis Zell. with the fastest time of about 4 days.

Uji Efektivitas Aplikasi Cendawan Entomopatogen Metarhizium anisopliae Terhadap Mortalitas Wereng Batang Coklat (Nilaparvata lugens Stal.)

“…Hasil percobaan ini memberikan nilai LC50 10 8 konidia/ml lebih besar dibandingkan dengan Novitasari (2023)yang menyatakan bahwa aplikasi cendawan M. anisopliae terhadap larva Aedes aegypti memberikan nilai analisis probit LC50 sebesar 1,98x10 6 konidia/ml. Pernyataan tersebut sama halnya dengan penelitian Pertiwi et al (2022)yang menghasilkan perhitungan LC50 yaitu sebesar 1x10 6 konidia/ml untuk patogenisitas cendawan M. anisopliae terhadap hama Crocidolomia binotalis. Menurut Tular et al (2022)semakin tinggi konsentrasi cendawan M. anisopliae yang diberikan pada serangga uji maka semakin cepat cendawan untuk menginfeksi serangga, dan dalam penelitiannya bahwa aplikasi cendawan M. anisopliae terhadap serangga kepinding tanah (Scotinophara coarctata) memperoleh nilai LC50 sebesar 1x10 7 konidia/ml.…”