This methodological study was planned to explore the validity and reliability of the evaluation scale for measuring the job motivation of nurses who work in psychiatric clinics. The sample was composed of 378 nurses who work in all psychiatric units or psychiatric hospitals located in Turkey's four large cities - Ankara, Istanbul, Izmir and Manisa. For testing reliability of 'job motivation scale', the internal consistency tests were executed with split scale analysis, Cronbach's alpha coefficient and item-total score correlation. For construct validity, factor analysis was used. For the first part of scale, Cronbach's alpha was determined to be 0.79. For the second part, Cronbach's alpha was 0.72. Factor analysis was performed in an attempt to establish validity and underlying associations between items in the scale. The first analysis produced nine eigenvalues (>1) and nine factors were extracted. The scree test indicated that a two-factor model would be suitable. The factor structure of the tool for measuring the job motivation of nurses who work in psychiatric clinics was parallel with motivation concepts. Validity and reliability levels of the scale for measuring the job motivation of nurses who work in psychiatric clinics were found to be sufficient in the Turkish population.