UK epidemic Escherichia coli strains A-E, with CTX-M-15  -lactamase, all belong to the international O25:H4-ST131 clone

Lau, Siu Ha; Kaufmann, Mary E.; Livermore, David M.; Woodford, Neil; Willshaw, G. A.; Cheasty, T.; Stamper, Katie; Reddy, Suganya; Cheesbrough, J.; Bolton, F. J.; Fox, Andrew; Upton, Mathew

doi:10.1093/jac/dkn380

Cited by 154 publications

(119 citation statements)

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“…Characterization of the bla genes revealed that half of the isolates are bla CTX-M-15 positive. This finding demonstrates the emergence of this ESBL type in the Netherlands and confirms previous studies that reported the emergence of this gene worldwide (1,2,10,20).…”

Section: Discussionsupporting

confidence: 91%

Rapid Typing of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae Isolates by Use of SpectraCell RA

et al. 2012

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Enterobacteriaceae are important pathogens of both nosocomial and community-acquired infections. In particular, strains with broad-spectrum beta-lactamases increasingly cause problems in health care settings. Rapid and reliable typing systems are key tools to identify transmission, so that targeted infection control measures can be taken. In this study, we evaluated the performance of Raman spectroscopic analysis (RA) for the typing of multiresistant Escherichia coli and Klebsiella pneumoniae isolates using the SpectraCell RA bacterial strain analyzer (River Diagnostics). Analysis of 96 unrelated isolates revealed that RA generated highly reproducible spectra and exhibited a discriminatory power that is comparable to pulsed-field gel electrophoresis. Furthermore, adequate results were obtained for three collections of clinical isolates. RA was able to discriminate outbreak-related isolates from isolates that were not involved in an outbreak or transmission. Furthermore, it was found that the RA approach recognized clones, irrespective of the extended-spectrum ␤-lactamase type. It can be concluded that RA is a suitable typing technique for E. coli and K. pneumoniae isolates. Combining high reproducibility, speed, and ease-of-use, this technique may play an important role in monitoring the epidemiology of these important nosocomial species.

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Section: Discussionsupporting

confidence: 91%

Rapid Typing of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae Isolates by Use of SpectraCell RA

et al. 2012

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“…Recent studies have demonstrated the clonal expansion of certain enterobacterial clones that are able to acquire plasmids harbouring different ESBLs and plasmidic AmpCs [8]. The high prevalence of bla ESBL genes in different parts of the world is caused by the horizontal transfer of plasmids among unrelated clones and also within local or international epidemic clones.…”

Section: Introductionmentioning

confidence: 99%

Dissemination of IncF plasmids carrying beta-lactamase genes in Gram-negative bacteria from Nigerian hospitals

Ogbolu

Daini

Ogunledun³

et al. 2013

J Infect Dev Ctries

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Introduction: Production of beta-lactamases is the predominant cause of resistance to beta-lactam antibiotics in Gram-negative bacteria. We investigated the diversity of plasmid-borne beta-lactamase genes and replicon type of the plasmids carrying the respective genes in Gramnegative bacteria recovered from clinical infection in Nigerian hospitals. Methodology: A total of 134 Gram-negative bacteria of 13 species were analyzed for antimicrobial susceptibility, phenotypic and genotypic detection of various beta-lactamases, and plasmid analysis, including replicon typing. Results: Of the 134 isolates, 111 (82.8%) contained beta-lactamases, while 28 (20.9%) carried extended-spectrum beta-lactamases. PCR and sequencing identified TEM-1 in 109 isolates (81.3%), SHV-1 in 33 isolates (24.6%), OXA-1 in 15 isolates (11.2%) and CTX-M enzymes (24 CTX-M-15 and 1 CTX-M-3) in 25 isolates (18.7%). Multiplex PCR showed that 6 isolates carried plasmidic AmpCs (ACT-1, DHA-1 and CMY-2); these enzymes were detected only in isolates possessing CTX-M beta-lactamases. Of 13 (76.9%) representative plasmids investigated in detail, 9 (69.2%) were self-transferable when selected by a beta-lactam and the plasmids once transferred coded for betalactam resistance. Replicon typing indicated IncF as the common vector encoding for beta-lactamases. Conclusions: The study showed a diversity of beta-lactamase genes disseminated by conjugative IncF plasmids in Gram-negative bacteria; TEM-1, SHV-1, OXA-1, CTX-M-15, CTX-M-3and plasmidic AmpC enzymes are in common circulation in Nigeria.

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“…The antibiotic resistance profiles of these STs differ markedly: members of STs 69, 73, and 95 remain largely susceptible to antibiotics and rarely have resistance to extended-spectrum cephalosporins in particular, whereas members of ST131 show increasing resistance to multiple antibiotic classes and account for 80 to 90% of multiresistant ExPEC infections (10,12,13). In particular, ST131 isolates often carry CTX-M-type extended-spectrum ␤-lactamases (ESBLs), together with fluoroquinolone and aminoglycoside resistances (13)(14)(15)(16).…”

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confidence: 99%

Rapid Identification of Major Escherichia coli Sequence Types Causing Urinary Tract and Bloodstream Infections

et al. 2015

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dEscherichia coli sequence types (STs) 69, 73, 95, and 131 are collectively responsible for a large proportion of E. coli urinary tract and bloodstream infections, and they differ markedly in their antibiotic susceptibilities. Here, we describe a novel PCR method to rapidly detect and distinguish these lineages. Three hundred eighteen published E. coli genomes were compared in order to identify signature sequences unique to each of the four major STs. The specificities of these sequences were assessed in silico by seeking them in an additional 98 genomes. A PCR assay was designed to amplify size-distinguishable fragments unique to the four lineages and was validated using 515 E. coli isolates of known STs. Genome comparisons identified 22 regions ranging in size from 335 bp to 26.5 kb that are unique to one or more of the four predominant E. coli STs, with two to 10 specific regions per ST. These regions predominantly harbor genes encoding hypothetical proteins and are within or adjacent to prophage sequences. Most (13/22) were highly conserved (>96.5% identity) in the genomes of their respective ST. The new assay correctly identified all 142 representatives of the four major STs in the validation set (n ‫؍‬ 515), with only two ST12 isolates misidentified as ST95. Compared with MLST, the assay has 100% sensitivity and 99.5% specificity. The rapid identification of major extraintestinal E. coli STs will benefit future epidemiological studies and could be developed to tailor antibiotic therapy to the different susceptibilities of these dominant lineages. Extraintestinal pathogenic Escherichia coli (ExPEC) strains are frequent pathogens, causing infections spanning a great range of severity (1, 2). They are responsible for 70 to 90% of acute community-acquired uncomplicated urinary infections, 85% of asymptomatic bacteriuria cases, and Ͼ60% of recurrent cystitis infections (3). E. coli is also one of the major pathogens of bloodstream infections, with mortality rates of 10 to 30%; in the United Kingdom, it has been the most common cause of bacteremia in most years since 1990, showing year-on year increases and now accounting for almost one-third of all bacteremias (see www.hpa .org.uk) (4). Successful treatment has been complicated by a rise in the prevalence of antibiotic-resistant strains.DNA profiling, e.g., by multilocus sequence typing (MLST), has advanced our understanding of ExPEC lineages, and several international studies have reported the predominance of sequence types (STs) 69, 73, 95, and 131 among large collections of ExPEC from human infections (5-8). In the United Kingdom, recent regional studies reported the consistent prevalences of these four STs among ExPEC from urinary and bloodstream infections. Collectively, they comprised 45% of the ExPEC strains from community and hospital urine samples recovered in 2007 to 2009 and 2007 to 2008 in Northwest (NW) England and the East Midlands, respectively, as well as 58% of those from bacteremias in northern England in 2010 to 2012 (9-11). The antibiotic ...

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UK epidemic Escherichia coli strains A-E, with CTX-M-15 -lactamase, all belong to the international O25:H4-ST131 clone

Cited by 154 publications

References 9 publications

Rapid Typing of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae Isolates by Use of SpectraCell RA

Rapid Typing of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Escherichia coli and Klebsiella pneumoniae Isolates by Use of SpectraCell RA

Dissemination of IncF plasmids carrying beta-lactamase genes in Gram-negative bacteria from Nigerian hospitals

Rapid Identification of Major Escherichia coli Sequence Types Causing Urinary Tract and Bloodstream Infections

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