Ulcerative Colitis: Ultrastructure of Interstitial Cells in Myenteric Plexus

Rumessen, Jüri Johannes; Vanderwinden, Jean Marie; Horn, Thomas

doi:10.3109/01913121003770701

Cited by 21 publications

(24 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other cellular features, including mitochondrial ultrastructure, were generally undisturbed. These changes were similar to the changes in ICC that we had previously found in patients resected for severe UC in which the most pronounced changes were seen in ICC-SMP as compared with myenteric ICC (Rumessen 1996;Rumessen et al 2010). We did not perform quantitative electron microscopy, and the small sample size precluded conclusions being made with regard to segmental variations or the influence of disease severity, disease duration or types of medication.…”

Section: Discussionsupporting

confidence: 89%

“…The treatment protocol of control and patient tissue was similar in all studies. The tissue was processed for transmission electron microscopy and studied as previously described (Rumessen 1994(Rumessen , 1996Rumessen et al 2009Rumessen et al , 2010. Immediately after resection, pieces of colon were immersed in a modified Karnovsky fixative of 8%+8% or 12%+12% glutaraldehyde (Taab, Berkshire, UK) and paraformaldehyde (Merck, Darmstadt, Germany) or a modified Zamboni fixative of 4%+4% glutaraldehyde and paraformaldehyde with picric acid (Merck) added from a saturated solution to give a final concentration of 0.18% in 0.1 M phosphate buffer (Merck) at pH 7.3.…”

Section: Methodsmentioning

confidence: 99%

“…The concept of a role of ICC in the pathogenesis of motility-related functional disturbances in inflammatory bowel disease (IBD) has been introduced in ulcerative colitis (UC; Rumessen 1996;Rumessen et al 2010) and further studies of experimental inflammation in animals have supported a key role of ICC in muscle dysfunction in gut inflammatory processes (Lu et al 1997;Huizinga et al 1998;Der et al 2000;Faussone-Pellegrini et al 2002;Sanders et al 2002;Wang et al 2002Wang et al , 2005Rumessen and Vanderwinden 2003;Kinoshita et al 2007;Farrugia 2008). In small intestinal CD, c-kit studies have suggested reduced numbers of some ICC populations (Porcher et al 2002;Ohlsson et al 2007;Wang et al 2007;Villanacci et al 2008) and one paper has described selective ultrastructural injury to ICC and nerves in the ileum of patients with CD (Wang et al 2007).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Crohn’s disease of the colon: ultrastructural changes in submuscular interstitial cells of Cajal

2010

View full text Add to dashboard Cite

Interstitial cells of Cajal (ICC) at the submuscular border of the human colon (ICC-SMP) are the proposed pacemaker cells of the musculature. In patients with Crohn's disease (CD) of the colon, ICC-SMP showed characteristic cytological changes from controls. The changes comprised secondary lysosomes in connection with lipid droplets and cytoplasmic vacuoles or multiple empty, confluent and often outbulging vacuoles merging with cisterns of granular endoplasmic reticulum and clusters of glycogen granules. These changes were most pronounced in patients with macroscopical mucosal inflammation but were also demonstrable in uninvolved colonic segments. Relationships of ICC to other cells were undisturbed. The changes were selective to ICC-SMP, as glial cells, muscle cells and fibroblast-like cells at the submuscular border showed no cytological alterations compared with controls. Varicosities of the submuscular plexus were often empty and dilated. Fibroblast-like cells selectively encased macrophages and mast cells. The cytological changes in ICC-SMP in CD are thus similar to changes seen in ulcerative colitis and may be of pathophysiological significance with regard to the motility and sensory disturbances seen in patients with CD.

show abstract

Section: Discussionsupporting

confidence: 89%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Crohn’s disease of the colon: ultrastructural changes in submuscular interstitial cells of Cajal

2010

View full text Add to dashboard Cite

show abstract

“…full-thickness) specimens that represent an optimal substrate to document possible neuromuscular colonic alterations. 34 Electron microscopic studies, for instance, revealed that in patients with severe UC, ultra-structural abnormalities of the pacemaker cells -the so-called interstitial cells of Cajal (ICC) -are present in the sub-muscular pacemaker region 35 and the myenteric area 36 of the colon. Subsequent immunohistochemical investigations documented that, compared with controls, colonic specimens of UC patients express cyclooxygenase 2 in the myenteric neurons, suggesting that this expression, through an increased prostaglandin synthesis at this site, may contribute to the dysmotilty seen in this condition.…”

Section: Colonic Motor Abnormalities In Uc: Studies On Full-thicknessmentioning

confidence: 99%

Colonic motility in ulcerative colitis

et al. 2014

View full text Add to dashboard Cite

Background: Inflammatory conditions affecting the gut may cause motility disturbances, and ulcerative colitis -one of the main disorders among the inflammatory bowel diseases -may display abnormal colonic motility. Aim: To review the abnormalities of the large bowel in ulcerative colitis, by considering the motility, laboratory (in vitro) and pathological studies dealing with this topic. Methods: A comprehensive online search of Medline and the Science Citation Index was carried out. Results: Patients with ulcerative colitis frequently display colonic motor abnormalities, including lack of contractility, an increase of propulsive contractile waves, an excessive production of nitric oxide, vasoactive intestinal polypeptide nerves, interleukin 1 beta, neurotensin, tachykinins levels and the weaker action of substance P, likely related to a neuromuscular dysfunction due to the inflammatory process. Conclusions: A better understanding of the pathophysiological grounds of altered colonic motility in ulcerative colitis may lead to a more in-depth knowledge of the accompanying symptoms and to better and more targeted therapeutic approaches.

show abstract

“…The structure and function of the ENS in functional and inflammatory disorders of the gastrointestinal (GI) tract has been described [10,11]. However, little is known about the structure of myenteric plexuses in patients with colorectal cancer (CRC).…”

Section: +mentioning

confidence: 99%

Ultrastructural characteristics of myenteric plexus in patients with colorectal cancer

Zauszkiewicz-Pawlak¹,

Godlewski²,

Kwiatkowski³

et al. 2017

Folia Histochem Cytobiol.

View full text Add to dashboard Cite

Introduction. It has been found previously that colorectal cancer (CRC) is accompanied by atrophy of myenteric plexuses (MPs) localized close to the tumor. The aim of the study was to compare ultrastructure of MPs localized in the unchanged part of the colon wall distant to CRC tumor with the ultrastructure of MPs in the vicinity of CRC tumor. Material and methods. The present study was conducted using post-operative material derived from 11 patients with CRC. Samples of colon wall were taken from the margin of cancer invasion and from a macroscopically unchanged segment of the large intestine, immediately fixed and processed according to the standard protocol for transmission electron microscopy studies. Results. In the MPs localized in the control part of colon wall the presence of numerous unmyelinated axons and cell bodies of neurons, interstitial cells of Cajal and enteroglial cells were observed. As compared to control samples, in the MPs located close to the tumor invasion, expansion of the extracellular matrix and myelin-like structures accompanying some nerve fibers were found. The appearance of mast and plasma cells was observed within MPs in the vicinity of CRC tumor. Sporadically, apoptotic cells were present inside the MPs. Conclusions. The presence of myelin-like structures and apoptotic cells within MPs located close to tumor invasion suggests that atrophy of MPs may be caused by factors released from CRC tumor.

show abstract

Ulcerative Colitis: Ultrastructure of Interstitial Cells in Myenteric Plexus

Cited by 21 publications

References 69 publications

Crohn’s disease of the colon: ultrastructural changes in submuscular interstitial cells of Cajal

Crohn’s disease of the colon: ultrastructural changes in submuscular interstitial cells of Cajal

Colonic motility in ulcerative colitis

Ultrastructural characteristics of myenteric plexus in patients with colorectal cancer

Contact Info

Product

Resources

About