Ulmus davidiana var. japonica Extracts Suppress Lipopolysaccharide-Induced Apoptosis Through Intracellular Calcium Modulation in U937 Macrophages

Abstract

This study was aimed to examine the antiapoptotic effect of Ulmus davidiana extracts through regulation of the intracellular cation mobilization in U937 human monocytic cells. To investigate the modulatory effects on lipopolysaccharide-induced apoptosis and the Ca2+ signaling pathway, we measured the levels of intracellular Ca2+ and various protein markers such as Bax, Bcl-2, and PARP. To isolate biopotent molecules, the branches of U. davidiana were processed sequentially using 60% ethanol, supercritical fluid extraction, and ethyl acetate extraction of the remaining samples to obtain single fractions and catechin-glycoside, which is one of the known bioeffector molecules of U. davidiana. Lipopolysaccharide increased intracellular Ca2+ mobilization in U937 cells by inducing transient oscillations and markedly increased Bax and PARP protein expression and decreased Bcl-2 expression. All U. davidiana and catechin-glycoside significantly reduced lipopolysaccharide-induced intracellular Ca2+ mobilization and downregulated apoptosis-related molecules. These results suggest that U. davidiana and catechin-glycoside may be useful for improving immune system function.

Ulmus davidiana 60% edible ethanolic extract for prevention of pericyte apoptosis in diabetic retinopathy

“…Next, we tried to find out which compound in U60E plays this role. In a previous study, we confirmed that C7A ( Supplementary Figure 2 ) is an important bioactive compound related to cell survival isolated from U. davidiana extract, and U60E also contains abundant C7A( 24 ). Therefore, we hypothesized that C7A contained in U60E is an important compound in preventing the decrease in cell viability of pericytes.…”

“…U. davidiana extract also prevents apoptosis in various cells, such as mouse embryonic fibroblast cells, mouse embryonic liver cells, and rat pheochromocytoma cells [10–12]. In addition, in a previous study, we confirmed that the C7A compound contained in U. davidiana extract had bioactivity related to cell survival, and U60E also contained C7A ( 24 ). In addition, C7A is known as a representative bioactive compound of U. davidiana extract ( 22 , 23 ).…”

Unsaturated Fatty Acids Complex Regulates Inflammatory Cytokine Production through the Hyaluronic Acid Pathway

“…Macrophages respond to pathogens and cause an inflammatory response, producing pro-inflammatory cytokines such as TNF-α, IL-6, and IL-1β. In addition, inflammatory factors such as NO produced by iNOS and PGE2 produced by COX-2 are also secreted [68,69].…”