Ulmus minor bark hydro-alcoholic extract ameliorates histological parameters and testosterone level in an experimental model of PCOS rats

Abstract

Objective. Polycystic ovary syndrome (PCOS) is a common and multifactorial disease associated with female factor infertility. Ulmus minor bark (UMB) is one of the medicinal plants used in Persian folklore as a fertility enhancer. In the current study, we aimed to elucidate the effect of UMB hydro-alcoholic extract on histological parameters and testosterone condition in an experimental model of PCOS rats.Methods. Thirty female rats were randomly divided into five groups: (1) control, (2) vehicle, (3) PCOS/50 mg [6 mg/kg dehydroepiandrosterone (DHEA) + 50 mg/kg UMB hydro-alcoholic extract], (4) PCOS/150 mg (6 mg/kg DHEA + 150 mg/kg UMB hydro-alcoholic extract), and (5) PCOS (6 mg/kg DHEA). All interventions were performed for 21 days. Afterwards, stereological analysis was done for determination of ovarian volume and follicle number. The serum level of testosterone was measured by ELISA kit.Results. UMB hydro-alcoholic extract improved the total number of the corpus luteum in the treatment groups when compared to the PCOS group (p<0.05). PCOS/150 mg and PCOS/50 mg groups showed significantly lower total number of the primordial, primary, and secondary follicles as well as testosterone level compared to the PCOS group (p<0.05). The total number of antral follicles and volume of ovary did not differ significantly between groups.Conclusion. UMB extract may be an effective and good alternative in improving PCOS histological and testosterone disturbances although further studies are warranted to confirm the safety of UMB plant in human.

Therapeutic Potential of Tannic Acid in the Management of Polycystic Ovarian Syndrome (PCOS) in Letrozole Induced Rat Model: A Histological and a Biochemical Study

“…When the LH/FSH ratio is elevated, the ovaries preferentially synthesize androgens. Therefore, PCOS-diagnosed women have higher levels of LH and T (36). In PCOS, ovulation does not occur due to low FSH and there is no progesterone production since granulosa cells cannot transform into luteinized granulosa cells (37).…”

Protective effects of vanillic acid on letrozole-induced polycystic ovarian syndrome: A comprehensive study in female wistar rats

“…These slides were observed under a magnification of 100× to record the major histopathological changes. The various developmental phases of ovary such as primary (marked as yellow arrow), secondary (white arrow), tertiary (green arrow), corpus luteum (pink arrow), and corpora haemorrhgaica (red arrow) were studied to determine the influence of various drug administration ( Hoseinpour, et al, 2019 ).…”

PCOS Animal Models: An Approach Induced By Dehydroepiandrosterone

“…83 out of 90 articles used subcutaneous way to produce PCOS. DHEA was injected by unknown way in 3 articles (48)(49)(50). While hypodermic injection was used in 2 articles (51, 52), intramuscular injection was used in one article (53).…”