Ultra‐bright and stimuli‐responsive fluorescent nanoparticles for bioimaging

Battistelli, Giulia; Cantelli, Andrea; Guidetti, Gloria; Manzi, Jeannette; Montalti, Marco

doi:10.1002/wnan.1351

Cited by 39 publications

(37 citation statements)

References 83 publications

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“…Quantum dots, which are colloidal semiconductor nanocrystals, and polymer dots, which are semiconducting polymer nanoparticles, offer brighter fluorescence emission and longer emission lifetimes compared to fluorescent dyes . Applications of fluorescent nanoparticles include single particle tracking, multicolor imaging, multiplex detection of analytes, and bioimaging . Metallic nanoparticles have great potential as biosensors, and for targeted drug delivery, diagnostic imaging and theranostics .…”

Section: Types Of Nanoparticlesmentioning

confidence: 99%

Cryo‐electron microscopy and cryo‐electron tomography of nanoparticles

Stewart

2016

WIREs Nanomed Nanobiotechnol

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Cryo-transmission electron microscopy (cryo-TEM or cryo-EM) and cryo-electron tomography (cryo-ET) offer robust and powerful ways to visualize nanoparticles. These techniques involve imaging of the sample in a frozen-hydrated state, allowing visualization of nanoparticles essentially as they exist in solution. Cryo-TEM grid preparation can be performed with the sample in aqueous solvents or in various organic and ionic solvents. Two-dimensional (2D) cryo-TEM provides a direct way to visualize the polydispersity within a nanoparticle preparation. Fourier transforms of cryo-TEM images can confirm the structural periodicity within a sample. While measurement of specimen parameters can be performed with 2D TEM images, determination of a three-dimensional (3D) structure often facilitates more spatially accurate quantization. 3D structures can be determined in one of two ways. If the nanoparticle has a homogeneous structure, then 2D projection images of different particles can be averaged using a computational process referred to as single particle reconstruction. Alternatively, if the nanoparticle has a heterogeneous structure, then a structure can be generated by cryo-ET. This involves collecting a tilt-series of 2D projection images for a defined region of the grid, which can be used to generate a 3D tomogram. Occasionally it is advantageous to calculate both a single particle reconstruction, to reveal the regular portions of a nanoparticle structure, and a cryo-electron tomogram, to reveal the irregular features. A sampling of 2D cryo-TEM images and 3D structures are presented for protein based, DNA based, lipid based, and polymer based nanoparticles. WIREs Nanomed Nanobiotechnol 2017, 9:e1417. doi: 10.1002/wnan.1417 For further resources related to this article, please visit the WIREs website.

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Section: Types Of Nanoparticlesmentioning

confidence: 99%

Cryo‐electron microscopy and cryo‐electron tomography of nanoparticles

Stewart

2016

WIREs Nanomed Nanobiotechnol

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“…In line with this effort, recent surge in nanomedicine, to a certain extent, has shed light on activatable systems which can endow highly specific imaging and on‐demand therapy . These systems are relied on the fact that tumor featured with uncontrolled growth always exhibits rapid angiogenesis with some characteristics, such as altered expression profiles of certain local enzymes, aberrant hypoxia, and acidic pH condition . Due to these commonalities existed in tumor microenvironment, responsive theranostics that can differentiate healthy and diseased tissue would be realizable and beneficial for tumor specific imaging and therapy.…”

Section: Introductionmentioning

confidence: 99%

Polyphenol‐Inspired Facile Construction of Smart Assemblies for ATP‐ and pH‐Responsive Tumor MR/Optical Imaging and Photothermal Therapy

Song

Dai

et al. 2017

Small

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Smart assemblies have attracted increased interest in various areas, especially in developing novel stimuli-responsive theranostics. Herein, commercially available, natural tannic acid (TA) and iron oxide nanoparticles (Fe O NPs) are utilized as models to construct smart magnetic assemblies based on polyphenol-inspired NPs-phenolic self-assembly between NPs and TA. Interestingly, the magnetic assemblies can be specially disassembled by adenosine triphosphate, which shows a stronger affinity to Fe O NPs than that of TA and partly replaces the surface coordinated TA. The disassembly can further be facilitated by the acidic environment hence causing the remarkable change of the transverse relaxivity and potent "turn-on" of fluorescence (FL) signals. Therefore, the assemblies for specific and sensitive tumor magnetic resonance and FL dual-modal imaging and photothermal therapy after intravenous injection of the assemblies are successfully employed. This work not only provides understandings on the self-assembly between NPs and polyphenols, but also will open new insights for facilely constructing versatile assemblies and extending their biomedical applications.

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“…366 Color centers in FNDs show a PL quantum yield that, in the case of NV defects, are unitary. 366 Color centers in FNDs show a PL quantum yield that, in the case of NV defects, are unitary.…”

Section: Brightness Of Si Qds and Fndsmentioning

confidence: 99%

Nanodiamonds and silicon quantum dots: ultrastable and biocompatible luminescent nanoprobes for long-term bioimaging

2015

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Fluorescence bioimaging is a powerful, versatile, method for investigating, both in vivo and in vitro, the complex structures and functions of living organisms in real time and space, also using super-resolution techniques. Being poorly invasive, fluorescence bioimaging is suitable for long-term observation of biological processes. Long-term detection is partially prevented by photobleaching of organic fluorescent probes. Semiconductor quantum dots, in contrast, are ultrastable, fluorescent contrast agents detectable even at the single nanoparticle level. Emission color of quantum dots is size dependent and nanoprobes emitting in the near infrared (NIR) region are ideal for low back-ground in vivo imaging. Biocompatibility of nanoparticles, containing toxic elements, is debated. Recent safety concerns enforced the search for alternative ultrastable luminescent nanoprobes. Most recent results demonstrated that optimized silicon quantum dots (Si QDs) and fluorescent nanodiamonds (FNDs) show almost no photobleaching in a physiological environment. Moreover in vitro and in vivo toxicity studies demonstrated their unique biocompatibility. Si QDs and FNDs are hence ideal diagnostic tools and promising non-toxic vectors for the delivery of therapeutic cargos. Most relevant examples of applications of Si QDs and FNDs to long-term bioimaging are discussed in this review comparing the toxicity and the stability of different nanoprobes.

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Ultra‐bright and stimuli‐responsive fluorescent nanoparticles for bioimaging

Cited by 39 publications

References 83 publications

Cryo‐electron microscopy and cryo‐electron tomography of nanoparticles

Cryo‐electron microscopy and cryo‐electron tomography of nanoparticles

Polyphenol‐Inspired Facile Construction of Smart Assemblies for ATP‐ and pH‐Responsive Tumor MR/Optical Imaging and Photothermal Therapy

Nanodiamonds and silicon quantum dots: ultrastable and biocompatible luminescent nanoprobes for long-term bioimaging

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