Ultra-dense polymer brush coating reduces Staphylococcus epidermidis biofilms on medical implants and improves antibiotic treatment outcome

Skovdal, Sandra M.; Jørgensen, Nis Pedersen; Petersen, Eskild; Jensen-Fangel, Søren; Ogaki, Ryosuke; Zeng, Gang; Johansen, Mikkel Illemann; Wang, Mikala; Rohde, Holger; Meyer, Rikke Louise

doi:10.1016/j.actbio.2018.07.002

Cited by 27 publications

(22 citation statements)

References 24 publications

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“…The results were in good agreement with the other literatures. [79,80] The formation of polyPEGMA brush on Ti alloy makes the surface hydrophilic, thereby preventing the hydrophobic interactions with the proteins present in bacterial cells and hence, very few cells were found to adhere on the brush modified surface. [81] Although, polyPEGMA is known to repel protein, it is also seen that it cannot entirely resist bacterial adhesion as PEG unit may involve nonproteinaceous adhesions during interactions.…”

Section: Bacteria Patterning By Exploiting Antiadherence Activity Of ...mentioning

confidence: 99%

Protein Patterning on Microtextured Polymeric Nanobrush Templates Obtained by Nanosecond Fiber Laser

Verma

Rana

Vidyasagar

et al. 2022

Macromolecular Bioscience

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Micropatterned polymer brushes have attracted attention in several biomedical areas, i.e., tissue engineering, protein microarray, biosensors, etc., for precise arrangement of biomolecules. Herein, a facile and scalable approach is reported to create microtextured polymer brushes with the ability to generate different type of protein patterns. Nanosecond fiber laser is exploited to generate micropatterns on poly(poly(ethylene glycol) methacrylate) (polyPEGMA) brush modified Ti alloy substrate. Surface initiated atom transfer radical polymerization is employed to grow PolyPEGMA brush (11–87 nm thick) on Ti alloy surface immobilized with initiator having an initiator density (σ*) of 1.5 initiators per nm2. Polymer brushes are then selectively laser ablated and their presence on nontextured area is confirmed by atomic force microscopy, fluorescence microscopy, and X‐ray photoelectron spectroscopy. Spatial orientation of biomolecules is first achieved by nonspecific protein adsorption on areas ablated by the laser, via physisorption. Further, patterned brushes of polyPEGMA are modified to activated ester that gives rise to protein conjugation specifically on nonlaser ablated brush areas. Moreover, the laser ablated brush modified patterned template is also successfully utilized for generating alternate patterns of bacteria. This promising technique can be further extended to create interesting patterns of several biomolecules which are of great interest to biomedical research community.

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Section: Bacteria Patterning By Exploiting Antiadherence Activity Of ...mentioning

confidence: 99%

Protein Patterning on Microtextured Polymeric Nanobrush Templates Obtained by Nanosecond Fiber Laser

Verma

Rana

Vidyasagar

et al. 2022

Macromolecular Bioscience

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“…Bacterial adhesion and biofilm formation have a great impact on the service life of medical devices. It is demonstrated that polymer brush coating can efficiently prevent the adhesion of bacteria on the surface [96][97][98][99][100][101][102][103][104][105][106][107][108]. For instance, Ibanescu et al found that both poly(2-hydroxy ethyl methacrylate) brush and poly(poly(ethylene glycol)methacrylate) brush exhibited strong anti-adhesion capability of Staphylococcus epidermidis [96].…”

Section: Infection Resistance Of Polymer Brush Modified Substratesmentioning

confidence: 99%

The Bioanalytical and Biomedical Applications of Polymer Modified Substrates

Sun

2022

Polymers

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Polymers with different structures and morphology have been extensively used to construct functionalized surfaces for a wide range of applications because the physicochemical properties of polymers can be finely adjusted by their molecular weights, polydispersity and configurations, as well as the chemical structures and natures of monomers. In particular, the specific functions of polymers can be easily achieved at post-synthesis by the attachment of different kinds of active molecules such as recognition ligand, peptides, aptamers and antibodies. In this review, the recent advances in the bioanalytical and biomedical applications of polymer modified substrates were summarized with subsections on functionalization using branched polymers, polymer brushes and polymer hydrogels. The review focuses on their applications as biosensors with excellent analytical performance and/or as nonfouling surfaces with efficient antibacterial activity. Finally, we discuss the perspectives and future directions of polymer modified substrates in the development of biodevices for the diagnosis, treatment and prevention of diseases.

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“…As summarized in Figure 2 , Skovdal et al showed that a coating of ultra-dense PEG on the surface of a titanium implant can reduce the adhesion of Staphylococcus aureus by 89–93%. In this manner, ultra-dense PEG coatings improved the treatment outcome for implant-associated infections in mice after 5 days [ 84 ]. However, this same adhesion blocking the action of PEG also hampers the adhesion of human cells, which would compromise osseointegration.…”

Section: Structural Enhancements and Experimental Designsmentioning

confidence: 99%

“…A key concept is using a coating that can release the drug(s) only when needed, to give enhanced antibacterial activity, using trigger responsive release systems [ 84 ]. Such coatings would be responsive to changes in the local microenvironment, such as specific biomolecules whose concentrations would rise, to initiate the release of the cargo.…”

Section: Structural Enhancements and Experimental Designsmentioning

confidence: 99%

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Microbial Decontamination and Antibacterial Activity of Nanostructured Titanium Dental Implants: A Narrative Review

et al. 2021

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Peri-implantitis is the major cause of the failure of dental implants. Since dental implants have become one of the main therapies for teeth loss, the number of patients with peri-implant diseases has been rising. Like the periodontal diseases that affect the supporting tissues of the teeth, peri-implant diseases are also associated with the formation of dental plaque biofilm, and resulting inflammation and destruction of the gingival tissues and bone. Treatments for peri-implantitis are focused on reducing the bacterial load in the pocket around the implant, and in decontaminating surfaces once bacteria have been detached. Recently, nanoengineered titanium dental implants have been introduced to improve osteointegration and provide an osteoconductive surface; however, the increased surface roughness raises issues of biofilm formation and more challenging decontamination of the implant surface. This paper reviews treatment modalities that are carried out to eliminate bacterial biofilms and slow their regrowth in terms of their advantages and disadvantages when used on titanium dental implant surfaces with nanoscale features. Such decontamination methods include physical debridement, chemo-mechanical treatments, laser ablation and photodynamic therapy, and electrochemical processes. There is a consensus that the efficient removal of the biofilm supplemented by chemical debridement and full access to the pocket is essential for treating peri-implantitis in clinical settings. Moreover, there is the potential to create ideal nano-modified titanium implants which exert antimicrobial actions and inhibit biofilm formation. Methods to achieve this include structural and surface changes via chemical and physical processes that alter the surface morphology and confer antibacterial properties. These have shown promise in preclinical investigations.

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Ultra-dense polymer brush coating reduces Staphylococcus epidermidis biofilms on medical implants and improves antibiotic treatment outcome

Cited by 27 publications

References 24 publications

Protein Patterning on Microtextured Polymeric Nanobrush Templates Obtained by Nanosecond Fiber Laser

Protein Patterning on Microtextured Polymeric Nanobrush Templates Obtained by Nanosecond Fiber Laser

The Bioanalytical and Biomedical Applications of Polymer Modified Substrates

Microbial Decontamination and Antibacterial Activity of Nanostructured Titanium Dental Implants: A Narrative Review

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