Ultra-efficient antimicrobial photodynamic inactivation system based on blue light and octyl gallate for ablation of planktonic bacteria and biofilms of Pseudomonas fluorescens

Shi, Yixuan; Jiang, Lai; Lin, Shan; Jin, Wengang; Gu, Qing; Chen, Yuewen; Zhang, Ke; Ettelaie, Rammile

doi:10.1016/j.foodchem.2021.131585

Cited by 25 publications

(22 citation statements)

References 36 publications

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“…It also reported that there was no or minor efect on the activity of Pseudomonas fuorescens (P. fuorescens) under sub-lethal conditions with treatment (octyl gallate). But these treatments could signifcantly inhibit bioflm formation of P. fuorescens [36].…”

Section: Efect Of Ka On Morphologicalmentioning

confidence: 99%

Evaluation of Antibacterial and Antibiofilm Properties of Kojic Acid against Aeromonas sobria and Staphylococcus saprophyticus

Qian

Lan

et al. 2023

Journal of Food Quality

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Biofilms composed of microbes and extracellular polymeric substances (EPSs) pose a significant risk to human health and lead to economic loss in the food industry. In this study, the antimicrobial and antibiofilm properties of kojic acid (KA) against Aeromonas sobria (A. sobria) and Staphylococcus saprophyticus (S. saprophyticus) were investigated by determining the leakage of DNA and protein, cell morphology, biofilm formation, the metabolic activity of biofilms, excretion of EPS, and biofilm architecture. The results indicated that the values of minimum inhibitory concentration (MIC) of A. sobria and S. saprophyticus after KA treatment were 0.4 mg/mL and 1.6 mg/mL, respectively. 1 × MIC KA showed unignorable antimicrobial activity against the two bacteria, leading to alterations in the bacterial physicochemical characteristics and cell death. Sub-MICs of KA can inhibit biofilm formation and decrease the metabolic activity and excretion of EPS, and these inhibition effects were in a dose-dependent manner. These results were further confirmed by the visual images obtained from scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Moreover, S. saprophyticus is more susceptible to KA in inhibiting biofilm formation, and for A. sobria, changes in the cell structure and the permeability of the cell membrane were more obvious. This research highlighted the antibacterial and antibiofilm activity of KA against A. sobria and S. saprophyticus.

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Section: Efect Of Ka On Morphologicalmentioning

confidence: 99%

Evaluation of Antibacterial and Antibiofilm Properties of Kojic Acid against Aeromonas sobria and Staphylococcus saprophyticus

Qian

Lan

et al. 2023

Journal of Food Quality

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“…In our previous research, OG (0.4 mM) + BL (420 nm, 210 mW/cm 2 ) reduced the viable cell number of P. fluorescens by up to >5 Log cfu/mL within 15 min, and the mature biofilms could be eliminated when they were exposed to OG and BL irradiation for 90 min. 13 In this scenario, it was noted that the introduction of KI to OG-mediated PDI could empower dramatically enhanced bactericidal performance (complete sterilization within 5 min). Moreover, the degree of microbial killing could be further corroborated by further regulating suitable parameters (OG, KI concentrations, and light dose).…”

Section: Ki-potentiated Og-mediated Pdi On Planktonicmentioning

confidence: 99%

“…Crystal violet assay was used to assess the different treatments on biofilm formation after 12 h at 37 °C. 13 A multimode detection platform quantified biofilms at 600 nm (SpectraMax i3X, Molecular Devices, California, USA). To ensure that the PDI did not affect bacterial activity before biofilm incubation, viable bacteria were counted before biofilm formation.…”

Section: Pdi Of Planktonic Bacteriamentioning

confidence: 99%

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Ultraefficient OG-Mediated Photodynamic Inactivation Mechanism for Ablation of Bacteria and Biofilms in Water Augmented by Potassium Iodide under Blue Light Irradiation

Shi,

Chen,

Zheng

et al. 2023

J. Agric. Food Chem.

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While photodynamic inactivation (PDI) has emerged as a novel sterilization strategy for drinking water treatment that recently attracted tremendous attention, its efficiency needs to be further improved. In this study, we aimed to clarify the ultraefficient mechanism by which potassium iodide (KI) potentiates octyl gallate (OG)-mediated PDI against bacteria and biofilms in water. When OG (0.15 mM) and bacteria were exposed to blue light (BL, 420 nm, 210 mW/cm2), complete sterilization (>7.5 Log cfu/mL of killing) was achieved by the addition of KI (250 mM) within only 5 min (63.9 J/cm2). In addition, at lower doses of OG (0.1 mM) with KI (100 mM), the biofilm was completely eradicated within 10 min (127.8 J/cm2). The KI-potentiated mechanism involves in situ rapid photogeneration of a multitude of reactive oxygen species, especially hydroxyl radicals (•OH), reactive iodine species, and new photocytocidal substances (quinone) by multiple photochemical pathways, which led to the destruction of cell membranes and membrane proteins, the cleavage of genomic DNA and extracellular DNA within biofilms, and the degradation of QS signaling molecules. This multitarget synergistic strategy provided new insights into the development of an environmentally friendly, safe, and ultraefficient photodynamic drinking water sterilization technology.

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“…Shi et al [ 51 ] used OG in conjunction with photodynamic inactivation (PDI) by blue light (BL) to eradicate bacteria and eliminate biofilms of Pseudomonas fluorescens . In this study, PDI operated through the interaction of applied BL, the exogenous photosensitizer OG, and oxygen to produce ROS within the cell, which destroy intracellular lipids, proteins, and nucleic acids, and kill the bacterium.…”

Section: Octyl Gallatementioning

confidence: 99%

Alkyl Gallates as Potential Antibiofilm Agents: A Review

et al. 2023

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Biofilms, which consist of microorganisms embedded in a polymer-rich matrix, contribute to a variety of infections and increase antimicrobial resistance. Thus, there is a constant need to develop new chemotherapeutic agents to combat biofilms. This review article focuses on the use of alkyl gallates, gallic acid and its esters (methyl, ethyl, propyl, butyl, hexyl, octyl, and dodecyl gallate), most of which are found in plants, to inhibit biofilm formation. The studies under review reveal that alkyl gallates have the capacity to prevent biofilm development and eradicate mature biofilms through mechanisms that suppress the synthesis of the extracellular polymeric matrix, inhibit quorum-sensing signaling, and alter the microbial cell membrane. The effects are stronger the greater the length of the alkyl chain. Moreover, the alkyl gallates’ preventive activity against biofilm formation occurs at doses below the minimum inhibitory concentration. More importantly, combining alkyl gallates with antimicrobials or blue-light irradiation produces a synergistic effect on the inhibition of biofilm formation that can be used to treat infections and overcome microbial resistance.

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Ultra-efficient antimicrobial photodynamic inactivation system based on blue light and octyl gallate for ablation of planktonic bacteria and biofilms of Pseudomonas fluorescens

Cited by 25 publications

References 36 publications

Evaluation of Antibacterial and Antibiofilm Properties of Kojic Acid against Aeromonas sobria and Staphylococcus saprophyticus

Evaluation of Antibacterial and Antibiofilm Properties of Kojic Acid against Aeromonas sobria and Staphylococcus saprophyticus

Ultraefficient OG-Mediated Photodynamic Inactivation Mechanism for Ablation of Bacteria and Biofilms in Water Augmented by Potassium Iodide under Blue Light Irradiation

Alkyl Gallates as Potential Antibiofilm Agents: A Review

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