Introduction: There have been many reports on athletes’ cardiovascular function, but there are very few methods used to evaluate the case. Objective: This article studies the relationship between sports fatigue and the cardiovascular response of athletes so that athletes can exercise and train more scientifically and safely. Methods: The paper selected some national athletes from 2017 to 2020 to carry out the VO2max, step test, YoYo test, 12-minute running, and other tests related to the cardiovascular function. Results: Athletes can promote the improvement of their cardiovascular function when they perform aerobic exercise training. Conclusion: Public physical education and amateur physical training should focus on the development of aerobic endurance. This can improve athletes’ cardiovascular function. Level of evidence II; Therapeutic studies - investigation of treatment results.