Ultra-fast cyclosporin A quantitation in whole blood by Laser Diode Thermal Desorption – Tandem Mass Spectrometry; comparison with High Performance Liquid Chromatography–Tandem Mass Spectrometry

Jourdil, Jean-François; Picard, Pierre; Meunier, Cécile; Auger, Serge; Stanke‐Labesque, Françoise

doi:10.1016/j.aca.2013.10.051

Cited by 28 publications

(21 citation statements)

References 30 publications

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“…Several techniques have been developed to increase the throughput of LC–MS/MS. Among them, UPLC is one of the most commonly used techniques . UPLC increases the throughput by shortening the LC run time while retaining the resolution by using smaller‐diameter separation media and very high pressure.…”

Section: Chromatography Methodsmentioning

confidence: 99%

“…Moreover, matrix effects were characterized to be below 20%. Jourdil et al developed laser diode thermal desorption coupled to an atmospheric pressure chemical ionization‐MS/MS, which represented a technological breakthrough that removes the chromatographic separation step, thereby significantly increasing the analytical throughput for the quantification of CsA in whole blood. The laser pattern was set to ramp from 0 to 45% laser power within 2 seconds.…”

Section: Chromatography Methodsmentioning

confidence: 99%

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Recent advances in analytical methods for the therapeutic drug monitoring of immunosuppressive drugs

Zhang

2017

Drug Testing and Analysis

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Therapeutic drug monitoring (TDM) of immunosuppressive drugs (ISDs) with a narrow therapeutic index is an increasingly popular tool for minimizing drug toxicity while maximizing the prevention of graft loss and organ rejection. This review focuses on trends regarding analytical methods for theTDM of ISDs since 2011. The five most commonly prescribed immunosuppressive medications are critically reviewed: cyclosporine A, tacrolimus, sirolimus (rapamycin), everolimus, and mycophenolic acid. This review introduces the general background of TDM and ISDs and presents the recent developments in using liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immunoassays for the TDM of ISDs. Finally, a future perspective for these analytical methods is briefly discussed.

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Section: Chromatography Methodsmentioning

confidence: 99%

Section: Chromatography Methodsmentioning

confidence: 99%

Recent advances in analytical methods for the therapeutic drug monitoring of immunosuppressive drugs

Zhang

2017

Drug Testing and Analysis

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“…Laser‐diode thermal desorption (LDTD), introduced in 2004, was specifically designed for high‐throughput laboratories and can analyze one sample in a few seconds and run up to 3840 samples in a single sequence. This technology has been applied to the analysis of diverse small organic molecules of clinical, food safety, and environmental interest.…”

Section: Introductionmentioning

confidence: 99%

Signal enhancement in laser diode thermal desorption‐triple quadrupole mass spectrometry analysis using microwell surface coatings

et al. 2019

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Laser‐diode thermal desorption (LDTD) is an ionization source usually coupled to triple quadrupole mass spectrometry (QqQMS) and specifically designed for laboratories requiring high‐throughput analysis. It has been observed that surface coatings on LDTD microwell plates can improve the sensitivity of the analysis of small polar molecules. The objective of the present study is to understand and quantify the effect of microwell surface coatings on signal intensity of small organic molecules of clinical, environmental, and forensic interest. Experiments showed that the peak areas of diclofenac, chloramphenicol, salicylic acid, and 11‐nor‐9‐carboxy‐Δ9‐tetrahydrocannabinol obtained by LDTD‐QqQMS increased by up to 3 orders of magnitude when using microwells coated with ethylenediaminetetraacetic acid (EDTA). Tests with different chelating agents and polytetrafluoroethylene as microwell surface coatings showed that nitrilotriacetic acid gave significantly higher peak areas for five out of the nine compounds that showed signal enhancement using chelating agents as coatings. Scanning electron microscopy studies of EDTA‐coated and uncoated microwells showed that analytes deposited in the former formed more uniform and thinner films than in the latter. The enhancement effect of surface coatings in LDTD‐QqQMS was explained mainly by the formation of homogenous and thinner layers of nanocrystals of analytes that are easier to desorb thermally than the layers formed when the analytes dry in direct contact with the bare stainless‐steel surface. Chemisorption of some analytes to the stainless‐steel surface of the microwell plate appeared to be a minor factor. Surface coatings widen the number of compounds analyzable by LDTD‐QqQMS and can also improve sensitivity and limits of detection.

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“…The LDTD-APCI ion source has been applied for qualitative and quantitative analyses of different compounds in environmental science [13][14][15][16] as well as in the food area [17][18][19]. More recently, the use of the LDTD-APCI ion source has gained importance in bioanalysis and several authors have demonstrated its benefits in the field of quantitative analysis of small molecules in various biological fluids [20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Laser diode thermal desorption atmospheric pressure chemical ionization tandem mass spectrometry applied for the ultra-fast quantitative analysis of BKM120 in human plasma

et al. 2014

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A sensitive and ultra-fast method utilizing the laser diode thermal desorption ion source using atmospheric pressure chemical ionization coupled to tandem mass spectrometry (LDTD-APCI-MS/MS) was developed for the quantitative analysis of BKM120, an investigational anticancer drug in human plasma. Samples originating from protein precipitation (PP) followed by salting-out assisted liquid-liquid extraction (SALLE) were spotted onto the LazWell™ plate prior to their thermal desorption and detection by tandem mass spectrometry in positive mode. The validated method described in this paper presents a high absolute extraction recovery (>90 %) for BKM120 and its internal standard (ISTD) [D8]BKM120, with precision and accuracy meeting the acceptance criteria. Standard curves were linear over the range of 5.00 to 2000 ng mL(-1) with a coefficient of determination (R (2)) >0.995. The method specificity was demonstrated in six different batches of human plasma. Intra- and inter-run precision as well as accuracy within ±20 % at the lower limit of quantification (LLOQ) and ±15 % (other levels) were achieved during a three-run validation for quality control (QC) samples. The post-preparative stability on the LazWell™ plate at room temperature was 72 h and a 200-fold dilution of spiked samples was demonstrated. The method was applied successfully to three clinical studies (n = 847) and cross-checked with the validated LC-ESI-MS/MS reference method. The sample analysis run time was 10 s as compared to 4.5 min for the current validated LC-ESI-MS/MS method. The resultant data were in agreement with the results obtained using the validated reference LC-ESI-MS/MS assay and the same pharmacokinetic (PK) parameters were calculated for both analytical assays. This work demonstrates that LDTD-APCI-MS/MS is a reliable method for the ultra-fast quantitative analysis of BKM120 which can be used to speed-up and support its bioanalysis in the frame of the clinical trials.

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Ultra-fast cyclosporin A quantitation in whole blood by Laser Diode Thermal Desorption – Tandem Mass Spectrometry; comparison with High Performance Liquid Chromatography–Tandem Mass Spectrometry

Cited by 28 publications

References 30 publications

Recent advances in analytical methods for the therapeutic drug monitoring of immunosuppressive drugs

Recent advances in analytical methods for the therapeutic drug monitoring of immunosuppressive drugs

Signal enhancement in laser diode thermal desorption‐triple quadrupole mass spectrometry analysis using microwell surface coatings

Laser diode thermal desorption atmospheric pressure chemical ionization tandem mass spectrometry applied for the ultra-fast quantitative analysis of BKM120 in human plasma

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