2017
DOI: 10.1016/j.bios.2017.05.016
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Ultra-fast electronic detection of antimicrobial resistance genes using isothermal amplification and Thin Film Transistor sensors

Abstract: A low cost thin-film transistor (TFT) nanoribbon (NR) sensor has been developed for rapid real-time detection of DNA amplification using an isothermal Recombinase Polymerase Amplification (RPA) method. The semiconductor chip measures DNA amplification through a pH change, rather than via fluorescence. The utility of the method was demonstrated by amplifying CTX-M and NDM, two genes that confer bacterial resistance to cephalosporins and carbapenems, respectively. It is shown that this approach provides extremel… Show more

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Cited by 52 publications
(39 citation statements)
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“…Branavan et al 198 developed a microfluidics that integrated RPA amplification and fluorescent signal detection for the detection of sexually transmitted infections; Renner et al 47 developed a degas-actuated microfluidics that integrated multiplex RPA amplification and fluorescent signal detection for the detection of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.) bacterial pathogens; and Hu et al 199 developed thin film transistor-based microfluidics that integrated RPA amplification and electronic signal detection for the detection of anti-microbial resistance genes. We note that all these three examples require the input of manually pre-mixed RPA reaction reagents.…”
Section: Microfluidic Integration Of Rpa Assaysmentioning
confidence: 99%
“…Branavan et al 198 developed a microfluidics that integrated RPA amplification and fluorescent signal detection for the detection of sexually transmitted infections; Renner et al 47 developed a degas-actuated microfluidics that integrated multiplex RPA amplification and fluorescent signal detection for the detection of ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.) bacterial pathogens; and Hu et al 199 developed thin film transistor-based microfluidics that integrated RPA amplification and electronic signal detection for the detection of anti-microbial resistance genes. We note that all these three examples require the input of manually pre-mixed RPA reaction reagents.…”
Section: Microfluidic Integration Of Rpa Assaysmentioning
confidence: 99%
“…A recent study demonstrated a Thin Film Transistor sensor for RPA that significantly accelerates readout time, using pH changes during DNA amplification as an electrical signal [52]. The molecular targets in that study are beta lactamases conferring resistance to cephalosporins and carbapenems, and detection was achieved within 2-5 min; however those data do not include tests for specificity of the assay nor measurement of antimicrobial resistance levels in the bacteria [52]. Nevertheless these results broadly support our finding that RPA is a superior approach to genomic antimicrobial resistance testing.…”
Section: Discussionmentioning
confidence: 99%
“…Amplified target DNA is hybridized with the bound probes and individual reactions are scored either using some electronic device, for example, a thin-film transistor sensor (Hu et al 2017) or by image analysis. This technique is based on the identification of the genetic material of a microorganism in environmental samples, rather than relying on growing the microbe, or using a microscope.…”
Section: Nucleic Acid-based Methodsmentioning
confidence: 99%