2011
DOI: 10.1016/j.jneumeth.2011.07.014
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Ultra fast in vivo microwave irradiation for enhanced metabolic stability of brain biopsy samples during HRMAS NMR analysis

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Cited by 16 publications
(40 citation statements)
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“…This provides a metabolite signature comparable to 1 H MRS metabolite stability measurements (de Graaf et al 2009) and validated by high resolution magic angle spinning NMR (Detour et al 2011). Importantly, the targeted metabolite profiling assays performed show the role of tissue preservation dependent on processing techniques.…”
Section: Discussionmentioning
confidence: 74%
“…This provides a metabolite signature comparable to 1 H MRS metabolite stability measurements (de Graaf et al 2009) and validated by high resolution magic angle spinning NMR (Detour et al 2011). Importantly, the targeted metabolite profiling assays performed show the role of tissue preservation dependent on processing techniques.…”
Section: Discussionmentioning
confidence: 74%
“…lung, muscle, kidney, liver and pancreas), even the short delay between brain isolation and fixation results in metabolic alterations under hypoxemic conditions (Griffin and Salek, 2007). Additionally, metabolites are degraded at different rates, which further complicate analysis (de Graaf, et al, 2009; Detour, et al, 2011). In particular, glucose and energy-linked phosphates are prone to degradation within seconds of mammalian death (Lowry, et al, 1964).…”
Section: Resultsmentioning
confidence: 99%
“…Fixation methods, such as funnel freezing or the application of microwaves on an intact live brain coupled to 1 H MRS validation, as used in this report, insure the efficient quenching of metabolism (Detour, et al, 2011). When metabolism quenching is performed correctly, the advantage of brain tissue over biofluid profiling is significant as limited passage of metabolites by BBB, and differences between the brain parenchyma and CSF, present major pitfalls for the use of biomarkers from biofluid analysis.…”
Section: Resultsmentioning
confidence: 99%
“…Heating tissue samples rapidly to 80–90°C for 1–2 s with pulsed microwaves inactivates enzyme activity within the tissue specimens and “freezes” the tissue's metabolic state for HRMAS analysis. No significant change was seen either in the metabolic profile from HRMAS measurement of samples at 37°C or in tissue histology following microwave radiation . The impacts of sample ischemia, prolonged spinning times, and freeze‐thawing of samples have also been studied.…”
Section: Improving Methodologiesmentioning
confidence: 99%