Ultra high performance liquid chromatography with ion‐trap <scp>TOF</scp>‐<scp>MS</scp> for the fast characterization of flavonoids in <i><scp>C</scp>itrus bergamia</i> juice

Sommella, Eduardo; Pepe, Giacomo; Pagano, Francesco; Tenore, Gian Carlo; Dugo, Paola; Manfra, Michele; Campiglia, Pietro

doi:10.1002/jssc.201300591

Cited by 20 publications

(17 citation statements)

References 36 publications

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“…Table reports concentration values (mg/L) of the compounds of our interest. Qualitative profile of the sample is in good agreement with literature data . Preliminary tests of purification were carried out on a small scale, to ensure the optimization of the process without a high consumption of reagents and resources.…”

Section: Resultssupporting

confidence: 76%

Multidimensional preparative liquid chromatography to isolate flavonoids from bergamot juice and evaluation of their anti-inflammatory potential

et al. 2015

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Important objectives of a high-performance liquid chromatography preparative process are: purity of products isolated, yield, and throughput. The multidimensional preparative liquid chromatography method used in this work was developed mainly to increase the throughput; moreover purity and yield are increased thanks to the automated collection of the molecules based on the intensity of a signal generated from the mass spectrometer detector, in this way only a specific product can be targeted. This preparative system allowed, in few analyses both in the first and second dimensions, the isolation of eight pure compounds present at very different concentration in the original sample with high purity (>95%) and yield, which showed how the system is efficient and versatile. Pure molecules were used to validate the analytical method and to test the anti-inflammatory and antiproliferative potential of flavonoids. The contemporary presence, in bergamot juice, of all the flavonoids together increases the anti-inflammatory effect with respect to the single compound alone.

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Section: Resultssupporting

confidence: 76%

Multidimensional preparative liquid chromatography to isolate flavonoids from bergamot juice and evaluation of their anti-inflammatory potential

et al. 2015

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“…The main flavonoids identified (mg/g) were Neohesperidin (105.27), Naringin (101.88), Melitidin (75.89), Neoeriocitrin (56.61) and Hesperetin (55.65). Quantitative values were obtained as the average of quintuplicate analyses and were in accordance with tipical BJ flavonoid profile reported in the literature [27], [32].…”

Section: Resultsmentioning

confidence: 57%

“…Chemical composition of BJe was investigated as previously described [32]. Briefly, BJe was solubilized in methanol to a concentration of 1 mg/mL, ultrasonicated and filtered by a 0.2 µm nylon membrane (Millipore, Milan, Italy).…”

Section: Methodsmentioning

confidence: 99%

Flavonoid Fraction of Bergamot Juice Reduces LPS-Induced Inflammatory Response through SIRT1-Mediated NF-κB Inhibition in THP-1 Monocytes

et al. 2014

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Plant polyphenols exert anti-inflammatory activity through both anti-oxidant effects and modulation of pivotal pro-inflammatory genes. Recently, Citrus bergamia has been studied as a natural source of bioactive molecules with antioxidant activity, but few studies have focused on molecular mechanisms underlying their potential beneficial effects. Several findings have suggested that polyphenols could influence cellular function by acting as activators of SIRT1, a nuclear histone deacetylase, involved in the inhibition of NF-κB signaling. On the basis of these observations we studied the anti-inflammatory effects produced by the flavonoid fraction of the bergamot juice (BJe) in a model of LPS-stimulated THP-1 cell line, focusing on SIRT1-mediated NF-κB inhibition. We demonstrated that BJe inhibited both gene expression and secretion of LPS-induced pro-inflammatory cytokines (IL-6, IL-1β, TNF-α) by a mechanism involving the inhibition of NF-κB activation. In addition, we showed that BJe treatment reversed the LPS-enhanced acetylation of p65 in THP-1 cells. Interestingly, increasing concentrations of Sirtinol were able to suppress the inhibitory effect of BJe via p65 acetylation, underscoring that NF-κB–mediated inflammatory cytokine production may be directly linked to SIRT1 activity. These results suggest that BJe may be useful for the development of alternative pharmacological strategies aimed at reducing the inflammatory process.

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“…Therefore compound 8 was identified as quercetin-3-Orutinoside, while compound 15 was quercetin-3-O-rha-glu. Triglycosides (2,6,7,20,25,26) Four peaks exhibited [M-H]ions at m/z 755. However, their accurate masses and element composition data were not exactly identical.…”

Section: Identification Of Flavonoid Glycosidesmentioning

confidence: 99%

“…While undergoing the quality analysis of natural products in a mixture, it is the fact that not all the standard compounds could be obtained. In recent years, high resolution mass spectrometry combined with UPLC provides a powerful on-line technique for natural product characterization in a complex mixture [7,8,9,10]. Hybrid linear ion trap -Orbitrap Elite mass spectrometer (LTQ-Orbitrap Elite) is one of the high-resolution analyzer, which enables the recorded m/z values of ions with accuracy up to four decimal points, and it allows achieving mass error better than 5 ppm.…”

Section: Introductionmentioning

confidence: 99%

Screening of Terpene Lactones and Flavonoid Glycosides in <i>gingko biloba</i> Capsule by UPLC- Orbitrap High Resolution MS, with Emphasis on Isomer Differentiation

Zhou¹,

Tang²,

Zeng³

et al. 2014

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In present study, a method basing on accurate mass and fragmentation pattern was designed to screen the terpene lactones and flavonoids in the methanol extract of Ginkgo biloba capsule powder. An UPLC with a hybrid linear ion trap-orbitrap mass spectrometer (UPLC-LTQ-Orbitrap Elite) was used. The chromatographic analysis of the sample was performed on a C 18 column (100 × 2.1 mm ID, particle size 1.9 μm) with gradient elution using methanol and water with 0.10% formic acid. Mass spectrometry was performed in the negative ESI mode. By comparison the accurate mass, element composition and fragment pattern, twenty-six compounds were identified, including 5 ginkgo terpene lactones (bilobalide, ginkgolide A, B, C and J) and 21 flavonoid glycosides. The major glycosides identified included: kaempferol-3-O-glu-glu-rha, kaempferol-7-O-rutinoside, isorhamnetin-3-Orutinoside, kaempferol-3-O-rha-glu, quercetin-3-O-glucoside, kaempferol-3-O-rha-glu-coumaroyl and 3'methoxymyricetin-3-O-rutinoside. The structural isomers were differentiated through the characteristic ions obtained from their MS/MS spectra. In addition, a compound 3,5,4'-trihydroxyflavone-3-O-glucoside, which was not reported in G. biloba before to our knowledge, was identified in the extract. The fragmentation pathways and characteristic product ions of flavonoid glycosides were shown and summarized for the rapid identification. The developed method was proved to be useful and precise for fast qualitative analysis of flavonoid compounds in mixture.

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Ultra high performance liquid chromatography with ion‐trap TOF‐MS for the fast characterization of flavonoids in Citrus bergamia juice

Cited by 20 publications

References 36 publications

Multidimensional preparative liquid chromatography to isolate flavonoids from bergamot juice and evaluation of their anti-inflammatory potential

Multidimensional preparative liquid chromatography to isolate flavonoids from bergamot juice and evaluation of their anti-inflammatory potential

Flavonoid Fraction of Bergamot Juice Reduces LPS-Induced Inflammatory Response through SIRT1-Mediated NF-κB Inhibition in THP-1 Monocytes

Screening of Terpene Lactones and Flavonoid Glycosides in <i>gingko biloba</i> Capsule by UPLC- Orbitrap High Resolution MS, with Emphasis on Isomer Differentiation

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