Ultra‐High Resolution Separation of Diastereomers on Carbon Adsorption Stationary Phases

Abstract

Reversed-phase adsorption liquid chromatography was used to successfully resolve diastereomers in a complex mixture of low molecular weight polystyrene oligomers. The separation was achieved by incorporating a two-dimensional liquid chromatography system where the polystyrene oligomers were transported from the first dimensional column, a C 18 , to an adsorbent phase in the second dimension, a carbon clad zirconia (CCZ) column. Using this system up to 46 diastereomers, each consisting of eight configurational repeat units, were able to be resolved in ultra high separations.

Targeted Isolations From Complex Samples Using Two-Dimensional Hplc. Part 1: Analytical Scale Analysis

“…This has been discussed in detail in previous works. [2,3] While for the most part 2DHPLC has been employed for high-resolution separations and sample profiling, the focus of the present study was to demonstrate how 2DHPLC operated in a heart cutting mode could be employed for the targeted isolation and purification of specific compounds from within a complex mixture. Here, we undertake the isolation of targeted components at an analytical scale analysis, but in second and third parts of this study, this was extended to the targeted isolation at the preparative scale.…”