Ultra-high sensitive analysis of 3-hydroxybenzo[a]pyrene in human urine using GC-APLI-MS

Richter-Brockmann, Sigrid; Dettbarn, G.; Jessel, Soenke; John, Andrea; Seidel, Albrecht; Achten, Christine

doi:10.1016/j.jchromb.2019.04.007

Cited by 4 publications

(4 citation statements)

References 46 publications

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“…In addition to the number of CPD, urinary cotinine concentration or TNE are commonly used as a biomarker of exposure to nicotine products and have been used for the classification of smoking status. Richter-Brockmann et al showed a positive correlation of urinary 3-OH-BaP concentration to CPD and cotinine, respectively (R 2 = 0.88 each) [31]. We could confirm the positive correlation between urinary 3-OH-BaP and smoking dose, measured as CPD or TNE in our study (Figure 5).…”

Section: Human Studysupporting

confidence: 85%

“…Richter-Brockmann et al followed a different approach by means of GC-MS using atmospheric pressure laser ionization (APLI). A higher sensitivity compared to our method was reported by the use of APLI, which apparently improved the ionization yields for the methyl ether of 3-OH-BaP with an LLOQ of 1.8 pg/L [31]. Additionally, ascorbic acid was added as an antioxidant before enzymatic cleavage to protect the resulting free 3-OH-BaP from oxidative decomposition during the following work-up and analysis-a procedure that has been established for other PAHs before [38].…”

Section: Methodsmentioning

confidence: 88%

“…In other recent studies analyzing 3-OH-BaP in the urine of non-smokers and smokers, higher concentrations were found in both groups. Concentrations in non-occupationally subjects (N = 4-7) were found in a range of <LOD to 820 pg/L for non-smokers and of 320 to 2150 pg/L for smokers [26,27,31]. Richter-Brockmann et al speculated that the addition of ascorbic acid may have led to higher concentrations due to improved stability of the analyte.…”

Section: Human Studymentioning

confidence: 99%

“…In order to cover not only occupational but also environmental exposure, including cigarette smoking, sensitivity in the pg/L-range is required. This can be achieved by optimizing the sample preparation, including derivatization steps [24][25][26][27], purification and concentration procedures [28][29][30], or by application of different ionization techniques, such as atmospheric pressure laser ionization (APLI) [31]. Thus, many of these methods are hampered by complex analytical procedures and specific/expensive equipment that can only be used to a limited extent in larger cohorts of human biomonitoring campaigns and clinical studies, respectively.…”

Section: Introductionmentioning

confidence: 99%

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A Sensitive LC–MS/MS Method for the Quantification of 3-Hydroxybenzo[a]pyrene in Urine-Exposure Assessment in Smokers and Users of Potentially Reduced-Risk Products

et al. 2021

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Benzo[a]pyrene (BaP), a human carcinogen, is formed during the incomplete combustion of organic matter such as tobacco. A suitable biomarker of exposure is the monohydroxylated metabolite 3-hydroxybenzo[a]pyrene (3-OH-BaP). We developed a sensitive LC–MS/MS (liquid chromatography coupled with tandem mass spectrometry) method for the quantification of urinary 3-OH-BaP. The method was validated according to the US Food and Drug Administration (FDA) guideline for bioanalytical method validation and showed excellent results in terms of accuracy, precision, and sensitivity (lower limit of quantification (LLOQ): 50 pg/L). The method was applied to urine samples derived from a controlled clinical study to compare exposure from cigarette smoking to the use of potentially reduced-risk products. Urinary 3-OH-BaP concentrations were significantly higher in smokers of conventional cigarettes (149 pg/24 h) compared to users of potentially reduced-risk products as well as non-users (99% < LLOQ in these groups). In conclusion, 3-OH-BaP is a suitable biomarker to assess the exposure to BaP in non-occupationally exposed populations and to distinguish not only cigarette smokers from non-smokers but also from users of potentially reduced-risk products.

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Section: Human Studysupporting

confidence: 85%

Section: Methodsmentioning

confidence: 88%

Section: Human Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Sensitive LC–MS/MS Method for the Quantification of 3-Hydroxybenzo[a]pyrene in Urine-Exposure Assessment in Smokers and Users of Potentially Reduced-Risk Products

et al. 2021

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Vorläufiger Leitwert für Benzo[a]pyren (B[a]P) in der Innenraumluft

2021

Bundesgesundheitsbl

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Polycyclic Aromatic Hydrocarbons (PAHs) and their Derivatives (O-PAHs, N-PAHs, OH-PAHs): Determination in Suspended Particulate Matter (SPM) – a Review

et al. 2021

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The aim of this paper is the presentation of the current state-of-the-art about the determination of polycyclic aromatic hydrocarbons (PAHs) and their oxidized forms originating from Suspended Particulate Matter (SPM) samples. The influence of SPM on health is twofold. SPM, as composed of small particles, is dangerous for the respiratory system. Additionally, SPM is a carrier of many hazardous compounds, particularly PAHs. Recently, several researches focus on the derivatives of PAHs, particularly nitro-, oxy- and hydroxy-PAHs, which are more dangerous than the parent PAHs. Both gas and high-performance liquid chromatography with various detection techniques are used to analyze both PAHs and their oxidized forms. Due to the appearance of these compounds in the environment, at a very low level, an analyte concentration step has to be applied prior to analysis. If GC and HPLC techniques are chiefly used as analytical tools for these analyses, the spectrum of analyte concentration procedures is very broad. Many analyte concentration techniques are proposed: from classic liquid-solid extractions, including Soxhlet technique, pressurized liquid extraction (ASE) or microwave oven (MWE) and sonic supported extraction to SPE techniques applications. However, one should remember that PAH determination methods are tools for solving the main problem, i.e., the evaluation the health hazard connected to the presence of SPM in air. Thus, the main drawback of several papers found in this review, i.e., the lack of information concerning limit of detection (LOD) of these methods makes their applicability very limited.

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Ultra-high sensitive analysis of 3-hydroxybenzo[a]pyrene in human urine using GC-APLI-MS

Cited by 4 publications

References 46 publications

A Sensitive LC–MS/MS Method for the Quantification of 3-Hydroxybenzo[a]pyrene in Urine-Exposure Assessment in Smokers and Users of Potentially Reduced-Risk Products

A Sensitive LC–MS/MS Method for the Quantification of 3-Hydroxybenzo[a]pyrene in Urine-Exposure Assessment in Smokers and Users of Potentially Reduced-Risk Products

Vorläufiger Leitwert für Benzo[a]pyren (B[a]P) in der Innenraumluft

Polycyclic Aromatic Hydrocarbons (PAHs) and their Derivatives (O-PAHs, N-PAHs, OH-PAHs): Determination in Suspended Particulate Matter (SPM) – a Review

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