Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays

Yang, Shieh‐Yueh; Chieh, Jen Jie; Wang, W. C.; Yu, Chien-Lin; Lan, C. B.; Chen, J. H.; Horng, Herng-Er; Hong, Chin Yih; Yang, Hong-Chang; Huang, Wei

doi:10.1016/j.jviromet.2008.07.025

Cited by 38 publications

(29 citation statements)

References 6 publications

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“…These methods are categorized based on the type of detection target: nucleic acid-based detection, virus isolation and identification, antigen detection, antibody detection, etc. (Alberini et al 2009;Chen et al 2008;Ho et al 2009;Moore et al 2010;Payungporn et al 2006;Xie et al 2006;Yang et al 2008) The most commonly used detection methods are nucleic acid-based detection, such as reverse-transcription PCR, Real-time PCR, RT-LAMP, antibody detection and antigen detection (Antarasena et al 2007;Deng et al 2011;He et al 2007;Kang et al 2010;Khurana et al 2011;Shahsavandi et al 2011;Yea et al 2010;Zhao et al 2010). However, most of these methods require specialized equipment and highly trained operators, and these detection processes are time consuming, thereby making them impractical for point-of-care (POCT) detection (Yager et al 2008 Quantum dots (QDs) have been broadly used for biological labeling and imaging due to their unique optical properties, i.e., a broad excitation spectrum and a narrow and symmetric emission peak (Lu et al 2011;Medintz et al 2005;Zhou et al 2011b).…”

Section: Introductionmentioning

confidence: 99%

Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

Yuan

Zhou

et al. 2016

Biosensors and Bioelectronics

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A quantum dot-based lateral flow immunoassay system (QD-LFIAS) was developed to simultaneously detect both influenza A virus subtypes H5 and H9. Water-soluble carboxyl-functionalized quantum dots (QDs) were used as fluorescent tags. The QDs were conjugated to specific influenza A virus subtype H5 and H9 antibodies via an amide bond. When influenza A virus subtype H5 or H9 was added to the QD-LFIAS, the QD-labeled antibodies specifically bound to the H5 or H9 subtype viruses and were then captured by the coating antibodies at test line 1 or 2 to form a sandwich complex. This complex produced a bright fluorescent band in response to 365 nm ultraviolet excitation. The intensity of fluorescence can be detected using an inexpensive, low-maintenance instrument, and the virus concentration directly correlates with the fluorescence intensity. The detection limit of the QD-LFIAS for influenza A virus subtype H5 was 0.016 HAU, and the detection limit of the QD-LFIAS for influenza A virus subtype H9 was 0.25 HAU. The specificity and reproducibility were good. The simple analysis step and objective results that can be obtained within 15 min indicate that this QD-LFIAS is a highly efficient test that can be used to monitor and prevent both Influenza A virus subtypes H5 and H9.

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Section: Introductionmentioning

confidence: 99%

Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

Yuan

Zhou

et al. 2016

Biosensors and Bioelectronics

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“…According to the type of detection target, these methods could be categorized into, for example, virus isolation and identification, nucleic acid-based detection, antigen detection, and antibody detection [3][4][5][6][7][8]. Most of these methods need demanding conditions and professional operations, and some of the detection processes is time-consuming.…”

Section: Open Access Research Articlementioning

confidence: 99%

Ultra Sensitive Detection of Influenza A Virus Based on Cdse/Zns Quantum Dots Immunoassay

Wu¹,

Mao²,

Liu³

et al. 2016

SOJB

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Open Access Research articlesevere outbreaks disease, and they can cause serious infections among people.To date, numerous analytical methods have being used to detect influenza A virus. According to the type of detection target, these methods could be categorized into, for example, virus isolation and identification, nucleic acid-based detection, antigen detection, and antibody detection [3][4][5][6][7][8]. Most of these methods need demanding conditions and professional operations, and some of the detection processes is time-consuming. For these reasons, some new biosensors based on nano materials such as gold nanoparticles, magnetic nano beads and quantum dots (QDs) have been coming to the forefront [9][10][11][12].Developing a sensitive, specific and fast biosensor for diagnosing the influenza virus at the early stages of infection is a challenge. Lateral flow immune assay method has been widely used in detection of infectious diseases with the advantages of rapid, easy to operate, and low cost [13]. However, traditional method such as colloidal gold lateral flow tests has limitations when high sensitivity is needed [14].QDs have been developed to replace colloidal gold owing to their excellent optical properties such as high fluorescence efficiency, wide range of excitation wavelength, narrow and symmetric emission spectra, and QDs based lateral flow tests have been proved higher sensitivity and reproducibility for detection of pathogen, characteristic protein and even nucleic acid [15][16][17][18].In this paper, we present an ultrasensitive lateral flow immunoassay (LFIA) for detecting influenza A virus. This immune sensor is based on site-specific covalent binding with the Fc end of influenza A virus antibodies to CdSe/ ZnS QDs. The carboxylfunctionalized CdSe/ ZnS QDs conjugated with antibodies via an amide bond often result in random links of the antibody structure in the QD conjugates and block certain antigenbinding sites. In order to obtain site-specific linking, we modified carboxyl-functionalized QDs with adipicdihydrazide (ADH) and oxidized the carbohydrate groups on the antibody's Fc region AbstractAn ultrasensitive lateral flow immunoassay system (LFIAS) was established for the detection of influenza A virus. In this LFIAS, hydrophilic dihydrazide-modified CdSe/ZnSquantom dots (QDs) were conjugated with specific antibodies and used as fluorescent labels, and a pair of matched anti-nucleoprotein of influenza A virus antibodies were used to form a sandwich immunoassay. The QDs were in conjugation with the fragment crystallizable region (Fc region) of specific influenza A virus antibodies through aldehydehydrazide covalent chemistry, conferring high sensitivity. The antibodies used for detection are specific for the most conserved and popular nucleoprotein of influenza A virus and ensure the accuracy and specificity. The QDs-LFIAS can analyze the nasal-pharyngeal swab samples through simple steps and get results within 15 min. Detection of nasal-pharyngeal swab samples makes it more rapid and conve...

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“…11,12 To achieve specific binding of magnetic nanoparticles onto CRP, anti-CRP is covalently coated onto magnetic nanoparticles, which are stably dispersed in phosphate-buffered solution (pH 7.2). Under the action of multiple AC magnetic fields, individual magnetic nanoparticles dispersed in a reagent rotate in response to applied AC magnetic fields.…”

Section: Immunomagnetic Reduction Assaymentioning

confidence: 99%

“…In order to take advantage of magnetic nanoparticles in clinical molecular assays, Fe 3 O 4 magnetic nanoparticles conjugated with anti-CRP antibody can be used as labeling markers for detecting CRP. Meanwhile, the method referred to as immunomagnetic reduction (IMR), 11,12 a type of magnetically labeled immunoassay, is utilized to detect and quantify CRP levels through reduction of magnetic signals due to the association between magnetic nanoparticles and CRP.…”

Section: Introductionmentioning

confidence: 99%

Use of immunomagnetic reduction for C-reactive protein assay in clinical samples

Chang

Lai

Lin

et al. 2012

IJN

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Background: Magnetic nanoparticles biofunctionalized with antibodies are able to recognize and bind to the corresponding antigens. In this work, anti-C-reactive protein (CRP) antibody was covalently conjugated onto the surface of magnetic nanoparticles to label CRP specifically in serum. Methods: The level of serum CRP was detected by immunomagnetic reduction (IMR) assay, which identifies the changes in the magnetic signal representing the level of interaction between antibody-conjugated magnetic nanoparticles and CRP proteins. To investigate the feasibility of IMR for clinical application, pure CRP solutions and 40 human serum samples were tested for IMR detection of CRP to characterize sensitivity, specificity, and interference. Results: In comparison with the immunoturbidimetry assay, the results of the IMR assay indicated higher sensitivity and had a high correlation with those of the current immunoturbidimetry assay. Conclusion:We have developed a novel and promising way to assay CRP in human serum using immunomagnetic reduction in clinical diagnosis.

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Ultra-highly sensitive and wash-free bio-detection of H5N1 virus by immunomagnetic reduction assays

Cited by 38 publications

References 6 publications

Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

Multiplexed detection of influenza A virus subtype H5 and H9 via quantum dot-based immunoassay

Ultra Sensitive Detection of Influenza A Virus Based on Cdse/Zns Quantum Dots Immunoassay

Use of immunomagnetic reduction for C-reactive protein assay in clinical samples

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