Ultra‐performance convergence chromatography for the quantitative determination of bioactive compounds in Aralia continentalis Kitagawa as quality control markers

Abstract

A rapid ultra-performance convergence chromatography method was developed for the quantitative determination of bioactive compounds in Aralia continentalis as quality control markers. Quantitative analysis indicated the presence of two major bioactive compounds: diterpenoid acids continentalic acid and kaurenoic acid. Using a Torus 1-aminoanthracene column, continentalic acid and kaurenoic acid were separated in less than 8 min. The method was validated with respect to precision, accuracy, and linearity according to the International Conference on Harmonization guidelines. The optimized method exhibited a good linear correlation (r > 0.996), excellent precision (RSD< 1.0%), and acceptable recoveries (99.97-100.26%). Limits of detection for continentalic acid and kaurenoic acid were 0.068 and 0.097 μg/mL, respectively, while their corresponding limits of quantitation were 0.207 and 0.295 μg/mL. The system performance of ultra-performance convergence chromatography was compared with that of conventional high-performance liquid chromatography with respect to analysis time and efficiency. The proposed method was found to be reliable and convenient for the quantitative analysis of continentalic acid and kaurenoic acid in A. continentalis from South Korea and A. pubescens from China. This study is expected to serve as a guideline for the quality control of Aralia continentalis.

Development and validation of ultra‐high performance supercritical fluid chromatography method for quantitative determination of six compounds in Guizhi Fuling capsule and tablet samples

“…Back pressure and column temperature both influence mobile phase density and then influence the eluting power [18, 19]. As for back pressure, it directly influences the eluting power of a supercritical fluid through changing the density of CO 2 [20, 21].…”

A New Method for Determination of Thymol and Carvacrol in Thymi herba by Ultraperformance Convergence Chromatography (UPC2)

“…We speculated that the separation of Thl and Cal may be significantly affected by the polarity of mobile phase B and the polarity of mobile phase B is slightly larger or smaller, which is not conducive to the separation of the two isomers. It is noteworthy that the back pressure directly influences the eluting power of a supercritical fluid by changing the density of supercritical carbon dioxide [26]. Hence, different values of back pressure (1700, 1800, 2000, and 2500 psi) were investigated.…”

“…More importantly, UPC 2 is an environmentally friendly analytical technique that employs dramatically reduced quantities of organic solvents compared to conventional chromatographic methods [23,24]. Nowadays, UPC 2 technology has been widely utilized in pharmaceutical analysis, and could serve as an alternative or complementary approach alongside HPLC and GC [25][26][27]. To the best of our knowledge, no studies have been reported on the use of UPC 2 for analyzing Thl and Cal in Thymi herba as QC markers.…”

Development and Validation of a High-Performance Liquid Chromatography Method for Quality Assessment of Oriental Medicine, Dokhwalgisaeng-Tang

“…Sci. 2021, 11, 7829 2 of 11 acid and benzoic acid) and monoterpenoids (albiflorin and paeoniflorin) from P. lactiflora, cardiac glycosides (neritaloside and odoroside H) from T. chinensis, miscellaneous (5-hydroxymethylfurfural) from R. alutinosa, phenylpropanoids (ferulic acid) and miscellaneous (senkyunolide A and (Z)-ligustilide) from C. officinale, triterpenoids (ginsenoside Rb1 and ginsenoside Rg1) from P. ginseng, triterpenoids (pachymic acid and polyporenic acid C) from P. cocos, steroids (ecdysterone) from A. bidentata, iridoids (geniposide and geniposidic acid) and lignans (pinoresinol diglucoside) from E. ulmoides, iridoids (gentiopicroside and loganic acid) from G. straminea, phenylpropanoids (methyleugenol and safrole) from A. heterotropoides, chromones (prim-O-glucosylcimifugin and 5-O-methylvisammioside) from S. divaricate, phenylpropanoids (cinnamic acid and cinnamaldehyde) from C. cassia, flavonoids (liquiritin and liquiritin apioside) and triterpenodis (glycyrrhizin) from G. uralensis, and phenols (6-gingerol and 6-shogaol) from Z. officinale [10][11][12][13][14][15][16][17][18][19][20][21][22][23][24][25].…”