Ultra-Performance Liquid Chromatography Technology and Applications

Ud, Neue; Kele, Marianna; Bunner, Bernard; Kromidas, A; Dourdeville, Tad A.; Mazzeo, Antonino; Es, Grumbach; Serpa, Susan; Te, Wheat; Hong, Paula; Gilár, Martin

doi:10.1201/9781420084542-c3

Cited by 21 publications

(14 citation statements)

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“…The outflow of the CMFR was continuously sampled by a scanning mobility particle sizer (TSI, 3936L22 SMPS, Wang and Flagan, 1989), by filter-based collection for off-line molecular analysis using an ultra-performance liquid chromatography coupled to an electrospray-ionization timeof-flight mass spectrometer (Waters, ACQUITY/LCT Premier XE UPLC-ESI-ToF-MS, Neue et al, 2010), and by an on-line particle-vaporization electron-impact high-resolution time-of-flight mass spectrometer (Aerodyne, HR-ToF-AMS, DeCarlo et al, 2006). The SMPS provided measurements of the number-diameter distribution of the particle population.…”

Section: Experimental Procedures and Measurementsmentioning

confidence: 99%

Particle mass yield from <i>β</i>-caryophyllene ozonolysis

Chen

McKinney

et al. 2012

Atmos. Chem. Phys.

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Abstract. The influence of second-generation products on the particle mass yield of β-caryophyllene ozonolysis was systematically tested and quantified. The approach was to vary the relative concentrations of first- and second-generation products by adjusting the concentration of ozone while observing changes in particle mass yield. For all wall-loss corrected organic particle mass concentrations Morg of this study (0.5 < Morg < 230 μg m−3), the data show that the particle-phase organic material was composed for the most part of second-generation products. For 0.5< Morg < 10 μg m−3, a range which overlaps with atmospheric concentrations, the particle mass yield was 10 to 20% and was not sensitive to ozone exposure, implying that the constituent molecules were rapidly produced at all investigated ozone exposures. In contrast, for Morg > 10 μg m−3 the particle mass yield increased to as high as 70% for the ultimate yield corresponding to the greatest ozone exposures. These differing dependencies on ozone exposure under different regimes of Morg are explained by a combination of the ozonolysis lifetimes of the first-generation products and the volatility distribution of the resulting second-generation products. First-generation products that have short lifetimes produce low-volatility second-generation products whereas first-generation products that have long lifetimes produce high-volatility second-generation products. The ultimate particle mass yield was defined by mass-based stoichiometric yields αi of α0 = 0.17 ± 0.05, α1 = 0.11 ± 0.17, and α2 = 1.03 ± 0.30 for corresponding saturation concentrations of 1, 10, and 100 μg m−3. Terms α0 and α1 had low sensitivity to the investigated range of ozone exposure whereas term α2 increased from 0.32 ± 0.13 to 1.03 ± 0.30 as the ozone exposure was increased. These findings potentially allow for simplified yet accurate parameterizations in air quality and climate models that seek to represent the ozonolysis particle mass yields of certain classes of biogenic compounds.

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Section: Experimental Procedures and Measurementsmentioning

confidence: 99%

Particle mass yield from <i>β</i>-caryophyllene ozonolysis

Chen

McKinney

et al. 2012

Atmos. Chem. Phys.

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“…For decades, this limit restricted the work of the analysts especially in research. Only after the year 2000, commercial pumps with a pressure limit of 600-1000 bar were available and the new term UHPLC was coined, with U for ultra (Wu and Clausen, 2007;Neue et al, 2010). (Actually, the first systems were developed by Waters, and this company created the term UPLC ® , which must not be used by other manufacturers.)…”

Section: Ultra High-performance Liquid Chromatographymentioning

confidence: 99%

High Pressure Liquid Chromatography and Ultra‐high Pressure Liquid Chromatography of Plants: Basic Concepts

Meyer

2014

Encyclopedia of Analytical Chemistry

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High‐performance liquid chromatography (HPLC) is an essential method in plant analysis. Although it is primarily a separation method, it offers also the possibility of on‐line quantitative analysis during the separation. It can be used for both, the investigation of classes of compounds as well as of individual substances. HPLC is an instrumental method of great flexibility with regard to the separation conditions and to the detection principle. In any case, high pressure is needed; conventional HPLC acts up to about 350 bar. As the available pressure limits the speed of analysis and its separation power, the more recent technology of ultra high‐performance liquid chromatography (UHPLC) works at pressures up to 1000 bar. It allows very fast analyses or high peak capacity (i.e., the number of resolved compounds) or a combination of both demands. The separation environment can be aqueous, for example, in reversed‐phase or ion‐exchange HPLC, or nonpolar with organic solvents in adsorption chromatography, thus allowing a great choice of investigative principles.

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“…The recently introduced UPLC is based on a chromatographic instrument capable of working with very high back pressures (up to 15,000 psi) generated by sub‐2 μm particle size columns (Plumb et al , ; Neue et al , ). This technology offers significant theoretical advantages in resolution, speed and sensitivity for analytical applications, particularly when coupled with mass spectrometers capable of high‐speed acquisitions.…”

Section: Lc Separationmentioning

confidence: 99%

Analyses of benzodiazepines and their metabolites in various biological matrices by LC-MS(/MS)

Nakamura

2011

Biomed. Chromatogr.

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Benzodiazepines are among the most frequently prescribed drugs due to their sedative, hypnotic, anxiolytic, muscle relaxant and antiepileptic properties. Because of the high consumption of benzodiazepines worldwide, this class of drugs and their metabolites are frequently present in both clinical and forensic cases. For these reasons, the analysis of benzodiazepines and their metabolites in biological fluids is of great interest to clinicians and forensic toxicologists. This paper reviews procedures for multi-analyte single-stage (LC-MS) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) using different mass analyzers for the screening, identification and/or quantification of drugs, poisons and/or their metabolites in blood, plasma, serum or urine published since 2001. Basic information about the biosamples assayed, work-up, LC column, mobile phase, ionization type, mass spectral detection mode, matrix effects and validation data for each procedure is summarized. The feasibility of using LC-MS(/MS) techniques to identify and quantify benzodiazepines and their metabolites is also discussed.

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Ultra-Performance Liquid Chromatography Technology and Applications

Cited by 21 publications

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Particle mass yield from <i>β</i>-caryophyllene ozonolysis

Particle mass yield from <i>β</i>-caryophyllene ozonolysis

High Pressure Liquid Chromatography and Ultra‐high Pressure Liquid Chromatography of Plants: Basic Concepts

Analyses of benzodiazepines and their metabolites in various biological matrices by LC-MS(/MS)

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Ultra-Performance Liquid Chromatography Technology and Applications

Cited by 21 publications

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Particle mass yield from &lt;i&gt;β&lt;/i&gt;-caryophyllene ozonolysis

Particle mass yield from &lt;i&gt;β&lt;/i&gt;-caryophyllene ozonolysis

High Pressure Liquid Chromatography and Ultra‐high Pressure Liquid Chromatography of Plants: Basic Concepts

Analyses of benzodiazepines and their metabolites in various biological matrices by LC-MS(/MS)

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Particle mass yield from <i>β</i>-caryophyllene ozonolysis

Particle mass yield from <i>β</i>-caryophyllene ozonolysis