Ultra-sensitive Colorimetric Method to Quantitate Hundreds of Polynucleotide Molecules by Gold Nanoparticles with Silver Enhancement

Li, Bosheng; Zhao, Liling; Zhang, Chi; Hei, Xiao-Han; Li, Fang; Li, Xiaobo; Shen, Jie; Li, Yuanyuan; Huang, Qiang; Xu, Shunqing

doi:10.2116/analsci.22.1367

Cited by 13 publications

(6 citation statements)

References 16 publications

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“…Thus one can easily deduced the amount of miRNA transfected into the given cell population, by estimating the amount of Au. The AuNP detection method by ICPMS is more sensitive and convenient than other detection methods 36 .…”

Section: Discussionmentioning

confidence: 99%

Breast cancer targeting novel microRNA-nanoparticles for imaging

et al. 2009

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MicroRNAs (miRNAs) are one of the most prevalent small (~22 nucleotide) regulatory RNA classes in animals. These miRNAs constitute nearly one percent of genes in the human genome, making miRNA genes one of the more abundant types of regulatory molecules. MiRNAs have been shown to play important roles in cell development, apoptosis, and other fundamental biological processes. MiRNAs exert their influence through complementary base-pairing with specific target mRNAs, leading to degradation or translational repression of the targeted mRNA. We have identified and tested a novel microRNA (miR-491) and demonstrated increased apoptosis in hepatocellular carcinoma cells (HepG2) and in human breast cancer cells (HBT3477) in vitro. We prepared a novel cancer targeting assembly of gold nanoparticles (GNP) with Quantum dots, miR-491, and MAb-ChL6 coupled through streptavidin/biotin for effective transfection, and to induce apoptosis in specific cancer cells for imaging and targeted therapy. The targeting and apoptosis inducing ability was tested by confocal and electron microscopy. The MAb-GNP-miR491-Qdot construct effectively transfected into the HBT3477 cells and induced apoptosis the confirmation of these results would suggest a new class of molecules for the imaging and therapy of breast cancer.

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Section: Discussionmentioning

confidence: 99%

Breast cancer targeting novel microRNA-nanoparticles for imaging

et al. 2009

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“…Thus, it is necessary to convert dsDNA to ssDNA by melting the duplex before hybridization. In this process, the probes and the degraded complementary sequences were competitively combined with the target ssDNA (30). Compared with the spiked target ssDNA, quantifying the dsDNA required the addition of excess reporter DNA probes (Sequence 2 labeled with AuNPs) and capture DNA probes.…”

Section: Analytical Performancementioning

confidence: 99%

Detection of Shigella flexneri DNA by ICP-MS Based on Oligonucleotide Hybridization and Labeling of Gold Nanoparticles

Wang¹,

Hu²

2017

At.Spectrosc.

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“…biotin-5'-AGACCTTTGACCCAGTTTTT-3'')(Juge-Aubryet al, 1997). The synthetic single-stranded probes were dissolved in sterile TE buffer (10 mM Tris-HCL, 1 mM EDTA, pH=8.0) and melted in annealing buffer (10 mM Tris-HCL, 1 mM EDTA, 50 mM NaCl, pH 8.0) at 72°C for 3 min, followed by cooling to 60°C for 1.5 min, further to 45°C for 1 min and 10°C for 3 min in a thermal cycler (Wang et al, 2003;Li et al, 2006).…”

Section: Preparation Of Biotin -Modified Probes For Pparα-rxrα Bindingmentioning

confidence: 99%

A rapid and high-throughput quantum dots bioassay for monitoring of perfluorooctane sulfonate in environmental water samples

Zhang

Wan

et al. 2011

Environmental Pollution

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Currently HPLC/MS is the state of the art tool for environmental/drinking water perfluorooctane sulfonate (PFOS) monitoring. PFOS can bind to peroxisomal proliferator-activated receptor-alpha (PPARα), which forms heterodimers with retinoid X receptors (RXRs) and binds to PPAR response elements. In this bioassay free PFOS in water samples competes with immobilized PFOS in ELISA plates for a given amount of PPARα-RXRα. It can be determined indirectly by immobilizing PPARα-RXRα-PFOS complex to another plate coated with PPARα antibody and subsequent measuring the level of PPARα-RXRα by using biotin-modified PPARα-RXRα probes-quantum dots-streptavidin detection system. The rapid and high-throughput bioassay demonstrated a detection limit of 2.5 ng L(-1) with linear range between 2.5 ng L(-1) and 75 ng L(-1). Detection results of environmental water samples were highly consistent between the bioassay and HPLC/MS.

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Ultra-sensitive Colorimetric Method to Quantitate Hundreds of Polynucleotide Molecules by Gold Nanoparticles with Silver Enhancement

Cited by 13 publications

References 16 publications

Breast cancer targeting novel microRNA-nanoparticles for imaging

Breast cancer targeting novel microRNA-nanoparticles for imaging

Detection of Shigella flexneri DNA by ICP-MS Based on Oligonucleotide Hybridization and Labeling of Gold Nanoparticles

A rapid and high-throughput quantum dots bioassay for monitoring of perfluorooctane sulfonate in environmental water samples

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