Ultra-sensitive immunoassay biosensors using hybrid plasmonic-biosilica nanostructured materials

Yang, Jing; Zhen, Le; Ren, Fanghui; Campbell, Jeremy; Rorrer, Gregory L.; Wang, Alan X.

doi:10.1002/jbio.201400070

Cited by 56 publications

(45 citation statements)

References 30 publications

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“…Compared with traditional porous substrates, the two dimensional (2-D) periodic pores on the 3-D diatom frustule with hierarchical nanoscale photonic crystal features can enhance the local optical fields at the surface of and inside diatom frustules (Jeffryes et al, 2008b; Yang et al, 2011). Our previous studies have shown that such unique photonic crystal features are capable of enhancing LSPRs of self-assembled metal NPs on the surface of diatom frustules, which will enable additional enhancement of SERS signals of the molecules adsorbed on the metallic NPs (Ren et al, 2014; Ren et al, 2013; Yang et al, 2014). In this work, we demonstrated a simple and fast method to integrate Ag NPs by an in-situ growth method from electroless deposited seeds within the photonic nanoporous diatom for the fabrication of 3-D hybrid plasmonic-photonic crystal biosilica SERS substrates.…”

Section: Introductionmentioning

confidence: 99%

Detecting explosive molecules from nanoliter solution: A new paradigm of SERS sensing on hydrophilic photonic crystal biosilica

Kong

Duff

et al. 2017

Biosensors and Bioelectronics

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We demonstrate a photonic crystal biosilica surface-enhanced Raman scattering (SERS) substrate based on a diatom frustule with in-situ synthesized silver nanoparticles (Ag NPs) to detect explosive molecules from nanoliter (nL) solution. By integrating high density Ag NPs inside the nanopores of diatom biosilica, which is not achievable by traditional self-assembly techniques, we obtained ultra-high SERS sensitivity due to dual enhancement mechanisms. First, the hybrid plasmonic-photonic crystal biosilica with three dimensional morphologies was obtained by electroless-deposited Ag seeds at nanometer sized diatom frustule surface, which provides high density hot spots as well as strongly coupled optical resonances with the photonic crystal structure of diatom frustules. Second, we discovered that the evaporation-driven microscopic flow combined with the strong hydrophilic surface of diatom frustules is capable of concentrating the analyte molecules, which offers a simple yet effective mechanism to accelerate the mass transport into the SERS substrate. Using the inkjet printing technology, we are able to deliver multiple 100 pico-liter (pL) volume droplets with pinpoint accuracy into a single diatom frustule with dimension around 30 μm × 7 μm × 5 μm, which allows for label-free detection of explosive molecules such as trinitrotoluene (TNT) down to 10−10 M in concentration and 2.7 × 10−15 g in mass from 120 nL solution. Our research illustrates a new paradigm of SERS sensing to detect trace level of chemical compounds from minimum volume of analyte using nature created photonic crystal biosilica materials.

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Section: Introductionmentioning

confidence: 99%

Detecting explosive molecules from nanoliter solution: A new paradigm of SERS sensing on hydrophilic photonic crystal biosilica

Kong

Duff

et al. 2017

Biosensors and Bioelectronics

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“…The sensitivity of the PL signal is enhanced by using a frustule that contains biogenically incorporated germanium, followed by thermal annealing [67]. The same group recently used a diatom frustule which was decorated with self-assembled Ag NP in a sandwich surface-enhanced Raman scattering (SERS) immunoassay to enhance the detection limit of mouse IgG in an ELISA to $100Â that of a conventional colloidal SERS sensor on flat glass [68].…”

Section: Biosensorsmentioning

confidence: 98%

Biogenic nanomaterials from photosynthetic microorganisms

Jeffryes

Agathos

Rorrer

2015

Current Opinion in Biotechnology

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“…This paper reports our most up-to-date research progress of diatom photonic crystal biosilica SERS substrates fabricated by self-assembling of gold nanoparticles (Au NPs) and in-situ growth of high density silver nanoparticles (Ag NPs), following our previous published work [29]–[31]. Specially, we studied the contribution of the diatom frustule to randomly distributed plasmonic NPs with high density hot-spots.…”

Section: Introductionmentioning

confidence: 99%

Chemical and Biological Sensing Using Diatom Photonic Crystal Biosilica With In-Situ Growth Plasmonic Nanoparticles

Kong

Squire

et al. 2016

IEEE Trans.on Nanobioscience

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In this paper, we described a new type of bioenabled nano-plasmonic sensors based on diatom photonic crystal biosilica with in-situ growth silver nanoparticles and demonstrated label-free chemical and biological sensing based on surface-enhanced Raman scattering (SERs) from complex samples. Diatoms are photosynthetic marine micro-organisms that create their own skeletal shells of hydrated amorphous silica, called frustules, which possess photonic crystal-like hierarchical micro-& nanoscale periodic pores. Our research shows that such hybrid plasmonic-biosilica nanostructures formed by cost-effective and eco-friendly bottom-up processes can achieve ultra-high limit of detection for medical applications, food sensing, water/air quality monitoring and geological/space research. The enhanced sensitivity comes from the optical coupling of the guided-mode resonance of the diatom frustules and the localized surface plasmons of the silver nanoparticles. Additionally, the nanoporous, ultra-hydrophilic diatom biosilica with large surface-to-volume ratio can concentrate more analyte molecules to the surface of the SERS substrates, which can help to detect biomolecules that cannot be easily adsorbed by metallic nanoparticles.

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Ultra-sensitive immunoassay biosensors using hybrid plasmonic-biosilica nanostructured materials

Cited by 56 publications

References 30 publications

Detecting explosive molecules from nanoliter solution: A new paradigm of SERS sensing on hydrophilic photonic crystal biosilica

Detecting explosive molecules from nanoliter solution: A new paradigm of SERS sensing on hydrophilic photonic crystal biosilica

Biogenic nanomaterials from photosynthetic microorganisms

Chemical and Biological Sensing Using Diatom Photonic Crystal Biosilica With In-Situ Growth Plasmonic Nanoparticles

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