Ultra-sensitive label-free SERS biosensor with high-throughput screened DNA aptamer for universal detection of SARS-CoV-2 variants from clinical samples

Park, Ki Sung; Choi, Anna; Kim, Hyun Jung; Park, In Soo; Eom, Mi-Suk; Yeo, Sang-Gu; Son, Ryeo Gang; Park, Tae‐In; Lee, Gyudo; Soh, H. Tom; Hong, Yoochan; Pack, Seung Pil

doi:10.1016/j.bios.2023.115202

Cited by 30 publications

(17 citation statements)

References 39 publications

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“…Specific interactions with plasma proteins (iv) must be elucidated during aptamer selection. Currently, several aptamer selections in the presence of plasma protein through in vivo strategy or negative selections have been gradually developed, − which was promising to facilitate the generation of an effective aptamer. In addition, the postselection strategies involving structural optimization, circular aptamer engineering, and covalent aptamer or conditionally activated aptamer constructions were considered to improve aptamer performance in the future. − …”

Section: Resultsmentioning

confidence: 99%

Investigation of the Relationship between Aptamers’ Targeting Functions and Human Plasma Proteins

Liu,

Ren,

Sun

et al. 2023

ACS Nano

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Section: Resultsmentioning

confidence: 99%

Investigation of the Relationship between Aptamers’ Targeting Functions and Human Plasma Proteins

Liu,

Ren,

Sun

et al. 2023

ACS Nano

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“…Furthermore, nucleic acid aptamers have higher thermal and pH stability than proteins, enabling conformational changes with reversible denaturation. Owing to these features, nucleic aptamers have attained interest as diagnostic and therapeutic tools [ 66 , 72 , 73 , 74 ].…”

Section: Aptamersmentioning

confidence: 99%

Aptamers and Nanobodies as New Bioprobes for SARS-CoV-2 Diagnostic and Therapeutic System Applications

Park,

Lee

et al. 2024

Biosensors

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The global challenges posed by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic have underscored the critical importance of innovative and efficient control systems for addressing future pandemics. The most effective way to control the pandemic is to rapidly suppress the spread of the virus through early detection using a rapid, accurate, and easy-to-use diagnostic platform. In biosensors that use bioprobes, the binding affinity of molecular recognition elements (MREs) is the primary factor determining the dynamic range of the sensing platform. Furthermore, the sensitivity relies mainly on bioprobe quality with sufficient functionality. This comprehensive review investigates aptamers and nanobodies recently developed as advanced MREs for SARS-CoV-2 diagnostic and therapeutic applications. These bioprobes might be integrated into organic bioelectronic materials and devices, with promising enhanced sensitivity and specificity. This review offers valuable insights into advancing biosensing technologies for infectious disease diagnosis and treatment using aptamers and nanobodies as new bioprobes.

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“…Stimuli-responsive polymers are a unique class of polymer materials that have recently gained much attention in the chemical and biological sensing applications because they can undergo a binding-induced conformational change, which is reminiscent of the folding of functional nucleic acids such as aptamers due to specific binding to the target molecules. − Linear chain biomolecules such as aptamers have been widely used as key recognition element in many biomedical applications, especially in biosensing, due to their high sensitivity, rapid response, real-time detection capability, and easy operation. − However, the screening of highly specific and selective aptamers in an acceptable period of time and the difficulty in recognizing small molecules remain as main challenges of aptamer-based biosensors . Hence, many efforts have been focused on finding alternative approaches to creating specific target receptors.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Detection of Glutamate and Histamine Using Redox-Labeled Stimuli-Responsive Polymer as a Synthetic Target Receptor

Ahmadian-Alam,

Andrade,

Song

2024

ACS Appl. Polym. Mater.

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Glutamate (Glu) and histamine (His) are two major neurotransmitters that play many critical roles in brain physiological functions and neurological disorders. Therefore, specific and sensitive monitoring of Glu and His is essential in the diagnosis and treatment of various mental health and neurodegenerative disorders. Both being nonelectroactive species, direct electrochemical detection of Glu and His has been challenging. Herein, we report a stimuli-responsive polymer-based biosensor for the electrochemical detection of Glu and His. The polymerbased target receptors consist of a linear chain stimuli-responsive templated polymer hybrid that is labeled with an osmium-based redox-active reporter molecule to elicit conformation-dependent electrochemical responses. The polymers are then attached to a gold electrode to implement an electrochemical sensor. The cyclic voltammetry (CV) and square-wave voltammetry (SWV) results confirmed the polymers' conformational changes due to the specific target (i.e., Glu and His) recognition and the corresponding electrochemical detection capabilities. The voltammetry results indicate that this biosensor can be used as a "signal-on" and "signal-off" sensors for the detection of Glu and His concentrations, respectively. The developed biosensor also showed excellent regeneration capability by fully recovering the initial current signal after being rinsed with deionized water. To further validate the polymer's utility as a target bioreceptor, the surface plasmon resonance (SPR) technique was used to characterize the binding affinity between the designed polymers and the target chemical. The SPR results exhibited the equilibrium dissociation constants (K D ) of 2.40 μM and 1.54 μM for the polymer−Glu and polymer−His interactions, respectively. The results obtained in this work strongly suggest that the proposed sensing technology could potentially be used as a platform for monitoring nonelectroactive neurochemicals from animal models.

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Ultra-sensitive label-free SERS biosensor with high-throughput screened DNA aptamer for universal detection of SARS-CoV-2 variants from clinical samples

Cited by 30 publications

References 39 publications

Investigation of the Relationship between Aptamers’ Targeting Functions and Human Plasma Proteins

Investigation of the Relationship between Aptamers’ Targeting Functions and Human Plasma Proteins

Aptamers and Nanobodies as New Bioprobes for SARS-CoV-2 Diagnostic and Therapeutic System Applications

Electrochemical Detection of Glutamate and Histamine Using Redox-Labeled Stimuli-Responsive Polymer as a Synthetic Target Receptor

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