Ultra-sensitive quantification of lysozyme based on element chelate labeling and capillary electrophoresis–inductively coupled plasma mass spectrometry

Yang, Mingwei; Wu, Weihua; Ruan, YaJuan; Huang, Limei; Wu, Zujian; Cai, Yong; Fu, FengFu

doi:10.1016/j.aca.2014.01.003

Cited by 18 publications

(10 citation statements)

References 35 publications

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“…These outstanding advantages make ICP-MS an attractive detector for the measurement of bioactive substances such as DNA, proteins, and cells by combining it with metallic labeling . In recent years, by combination with various metallic labeling techniques, ICP-MS has been used for the sensitive quantification of peptides, proteins, , DNA, , and even living organisms including bacteria and cancer cells. − However, the ICP-MS-based methods previously reported for the cancer cell detection suffer from one or more of the following shortages, such as relatively poor stability and high cost due to the usage of antibody (as recognition probe), and single-cell detection. − A DNA aptamer that was designed through an in vitro selection process can bind a number of specific molecular targets including cells with high affinity and specificity . In comparison with an antibody, the aptamer offers distinct advantages, such as higher stability, lower cost, ease of modification, nontoxicity, and a lack of immunogenicity .…”

mentioning

confidence: 99%

Combination of Magnetic-Beads-Based Multiple Metal Nanoparticles Labeling with Hybridization Chain Reaction Amplification for Simultaneous Detection of Multiple Cancer Cells with Inductively Coupled Plasma Mass Spectrometry

Chen

Huang

et al. 2018

Anal. Chem.

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The high-throughput and rapid screening of cancer cells in blood is one of the most effective ways to realize clinical early diagnosis of cancer. We herein developed an inductively coupled plasma mass-spectrometry (ICP-MS)-based method for simultaneously counting two cancer cells, human hepatocellular carcinoma cell (SMMC-7721) and human lung carcinoma cell (A549), with high sensitivity and specificity. The method employed a magnetic-beads (MBs)-based dual aptamers–dual metal nanoparticles labeling technique for simultaneously recognizing and labeling different metal nanoparticles (AuNPs and AgNPs) on SMMC-7721 and A549 cancer cells, respectively, to generate ICP-MS signals, and a DNA hybridization chain reaction strategy for realizing “one SMMC-7721 cell-to-massive AuNPs” and “one A549 cell-to-massive AgNPs” amplification effect to improve sensitivity. The employment of ICP-MS detection and MBs not only simplified the experimental operation but also greatly enhanced the resistance of the method to the complicated matrix. The method can be used to simultaneously detect as few as 50 SMMC-7721 and A549 cancer cells in serum within 1 h with a recovery of 93–108% and a relative standard deviation (RSD, n = 5) < 5%. The method has notable advantages such as high sensitivity, excellent stability, high throughput, shorter analysis time, and strong resistibility to the complex matrix. Especially, the method can also be used to detect various cancer cells via altering aptamers and designing appropriate link/signal probes according to the target cells. The success of this study offers a potential approach for the high-throughput and rapid screening of cancer cells in clinical early diagnosis of cancer.

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mentioning

confidence: 99%

Combination of Magnetic-Beads-Based Multiple Metal Nanoparticles Labeling with Hybridization Chain Reaction Amplification for Simultaneous Detection of Multiple Cancer Cells with Inductively Coupled Plasma Mass Spectrometry

Chen

Huang

et al. 2018

Anal. Chem.

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“…A CE‐ICP‐MS method was developed for quantifications of Zn‐, Cu‐, and Mn‐glycinates complexes in vitro and in vivo to assess their bioaccessibility in supplemented feed samples . An extremely low LOD of 3.89 attomole was attained in the quantification of lysozyme in a saliva sample . The proposed method based on the Gd 3+ chelate labeling provides a new possibility for biological assays and clinical diagnoses.…”

Section: Development Of Methodology and Instrumentationmentioning

confidence: 99%

Recent advances in combination of capillary electrophoresis with mass spectrometry: Methodology and theory

Klepárnı́k

2014

Electrophoresis

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This review focuses on the latest development of microseparation electromigration methods in capillaries and microfluidic devices with MS detection and identification. A wide selection of 183 relevant articles covers the literature published from June 2012 till May 2014 as a continuation of the review article on the same topic by Kleparnik [Electrophoresis 2013, 34, 70-86]. Special attention is paid to the new improvements in the theory of instrumentation and methodology of MS interfacing with capillary versions of zone electrophoresis, ITP, and IEF. Ionization methods in MS include ESI, MALDI, and ICP. Although the main attention is paid to the development of instrumentation and methodology, representative examples illustrate also applications in the proteomics, glycomics, metabolomics, biomarker research, forensics, pharmacology, food analysis, and single-cell analysis. The combinations of MS with capillary versions of electrochromatography and micellar electrokinetic chromatography are not included.

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“…A similar method was applied for detecting trace amounts of lysozyme, employing Gd‐chelated by DTPA as the tag . Lysozyme present in serum and saliva has been associated with breast cancer , which attracted Yang et al.…”

Section: Biological Inorganic Chemistry Applicationsmentioning

confidence: 99%

“…Lysozyme present in serum and saliva has been associated with breast cancer , which attracted Yang et al. to develop an ultrasensitive method for its quantification . The reported LOD of lysozyme was in the attomole range, with an RSD <6% and a sample recovery ranging from 91 to 106%.…”

Section: Biological Inorganic Chemistry Applicationsmentioning

confidence: 99%

Electrophoretic separation techniques and their hyphenation to mass spectrometry in biological inorganic chemistry

2016

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Electrophoretic methods have been widely applied in research on the roles of metal complexes in biological systems. In particular, CE, often hyphenated to a sensitive MS detector, has provided valuable information on the modes of action of metal-based pharmaceuticals, and more recently new methods have been added to the electrophoretic toolbox. The range of applications continues to expand as a result of enhanced CE-to-MS interfacing, with sensitivity often at picomolar level, and evolved separation modes allowing for innovative sample analysis. This article is a followup to previous reviews about CE methods in metallodrug research (Electrophoresis, 2003, 24, 2023-2037; Electrophoresis, 2007, 28, 3436-3446; Electrophoresis, 2012, 33, 622-634), also providing a comprehensive overview of metal species studied by electrophoretic methods hyphenated to MS. It highlights the latest CE developments, takes a sneak peek into gel electrophoresis, traces biomolecule labeling, and focuses on the importance of early-stage drug development.

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Ultra-sensitive quantification of lysozyme based on element chelate labeling and capillary electrophoresis–inductively coupled plasma mass spectrometry

Cited by 18 publications

References 35 publications

Combination of Magnetic-Beads-Based Multiple Metal Nanoparticles Labeling with Hybridization Chain Reaction Amplification for Simultaneous Detection of Multiple Cancer Cells with Inductively Coupled Plasma Mass Spectrometry

Combination of Magnetic-Beads-Based Multiple Metal Nanoparticles Labeling with Hybridization Chain Reaction Amplification for Simultaneous Detection of Multiple Cancer Cells with Inductively Coupled Plasma Mass Spectrometry

Recent advances in combination of capillary electrophoresis with mass spectrometry: Methodology and theory

Electrophoretic separation techniques and their hyphenation to mass spectrometry in biological inorganic chemistry

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